7SSA

Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A.1	polymer	131	13882.0	2	UniProt (P04911) Pfam (PF00125) Pfam (PF16211)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
4	D, H (D, H)	Histone H2B.1	polymer	130	14149.2	2	UniProt (P02293) Pfam (PF00125)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Suppressor of Ty protein 12
5	I, J (K, L)	Centromere-binding protein 1	polymer	352	39457.6	2	UniProt (P17106) Pfam (PF00010)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	CBP-1,Centromere promoter factor 1,Centromere-binding factor 1
6	K (I)	DNA (137-MER)	polymer	149	45796.2	1		synthetic construct
7	L (J)	DNA (137-MER)	polymer	149	46187.4	1		synthetic construct

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	variant

K, L: 2 - 351 (UniProt: P17106)

PDB	External Database	Details
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	280357.9
All*	Total formula weight	280357.9

*Water molecules are not included.