7SSA
Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E | Histone H3.2 | polymer | 135 | 15303.9 | 2 | UniProt (P84233) Pfam (PF00125) In PDB | Xenopus laevis (African clawed frog) | |
| 2 | B, F | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) In PDB | Xenopus laevis (African clawed frog) | |
| 3 | C, G | Histone H2A.1 | polymer | 131 | 13882.0 | 2 | UniProt (P04911) Pfam (PF00125) Pfam (PF16211) In PDB | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 4 | D, H | Histone H2B.1 | polymer | 130 | 14149.2 | 2 | UniProt (P02293) Pfam (PF00125) In PDB | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Suppressor of Ty protein 12 |
| 5 | K, L | Centromere-binding protein 1 | polymer | 352 | 39457.6 | 2 | UniProt (P17106) Pfam (PF00010) In PDB | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | CBP-1,Centromere promoter factor 1,Centromere-binding factor 1 |
| 6 | I | DNA (137-MER) | polymer | 149 | 45796.2 | 1 | synthetic construct | ||
| 7 | J | DNA (137-MER) | polymer | 149 | 46187.4 | 1 | synthetic construct |
Sequence modifications
A, E: 1 - 135 (UniProt: P84233)
K, L: 2 - 351 (UniProt: P17106)
| PDB | External Database | Details |
|---|---|---|
| Ala 102 | Gly 103 | variant |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 280357.9 | |
| All* | Total formula weight | 280357.9 |
