Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7SSA

Cryo-EM structure of pioneer factor Cbf1 bound to the nucleosome

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000786	cellular_component	nucleosome
A	0003677	molecular_function	DNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0030527	molecular_function	structural constituent of chromatin
A	0046982	molecular_function	protein heterodimerization activity
B	0000786	cellular_component	nucleosome
B	0003677	molecular_function	DNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005694	cellular_component	chromosome
B	0006334	biological_process	nucleosome assembly
B	0030527	molecular_function	structural constituent of chromatin
B	0046982	molecular_function	protein heterodimerization activity
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000786	cellular_component	nucleosome
C	0003677	molecular_function	DNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005694	cellular_component	chromosome
C	0006281	biological_process	DNA repair
C	0006325	biological_process	chromatin organization
C	0030527	molecular_function	structural constituent of chromatin
C	0031298	cellular_component	replication fork protection complex
C	0031492	molecular_function	nucleosomal DNA binding
C	0031507	biological_process	heterochromatin formation
C	0032991	cellular_component	protein-containing complex
C	0046982	molecular_function	protein heterodimerization activity
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0000786	cellular_component	nucleosome
D	0003677	molecular_function	DNA binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005694	cellular_component	chromosome
D	0006301	biological_process	postreplication repair
D	0006325	biological_process	chromatin organization
D	0006355	biological_process	regulation of DNA-templated transcription
D	0030527	molecular_function	structural constituent of chromatin
D	0031298	cellular_component	replication fork protection complex
D	0032991	cellular_component	protein-containing complex
D	0046982	molecular_function	protein heterodimerization activity
E	0000786	cellular_component	nucleosome
E	0003677	molecular_function	DNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005694	cellular_component	chromosome
E	0030527	molecular_function	structural constituent of chromatin
E	0046982	molecular_function	protein heterodimerization activity
F	0000786	cellular_component	nucleosome
F	0003677	molecular_function	DNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005694	cellular_component	chromosome
F	0006334	biological_process	nucleosome assembly
F	0030527	molecular_function	structural constituent of chromatin
F	0046982	molecular_function	protein heterodimerization activity
G	0000122	biological_process	negative regulation of transcription by RNA polymerase II
G	0000786	cellular_component	nucleosome
G	0003677	molecular_function	DNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005694	cellular_component	chromosome
G	0006281	biological_process	DNA repair
G	0006325	biological_process	chromatin organization
G	0030527	molecular_function	structural constituent of chromatin
G	0031298	cellular_component	replication fork protection complex
G	0031492	molecular_function	nucleosomal DNA binding
G	0031507	biological_process	heterochromatin formation
G	0032991	cellular_component	protein-containing complex
G	0046982	molecular_function	protein heterodimerization activity
H	0000122	biological_process	negative regulation of transcription by RNA polymerase II
H	0000786	cellular_component	nucleosome
H	0003677	molecular_function	DNA binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005694	cellular_component	chromosome
H	0006301	biological_process	postreplication repair
H	0006325	biological_process	chromatin organization
H	0006355	biological_process	regulation of DNA-templated transcription
H	0030527	molecular_function	structural constituent of chromatin
H	0031298	cellular_component	replication fork protection complex
H	0032991	cellular_component	protein-containing complex
H	0046982	molecular_function	protein heterodimerization activity
K	0000122	biological_process	negative regulation of transcription by RNA polymerase II
K	0000775	cellular_component	chromosome, centromeric region
K	0000776	cellular_component	kinetochore
K	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
K	0001227	molecular_function	DNA-binding transcription repressor activity, RNA polymerase II-specific
K	0001228	molecular_function	DNA-binding transcription activator activity, RNA polymerase II-specific
K	0003677	molecular_function	DNA binding
K	0003700	molecular_function	DNA-binding transcription factor activity
K	0005634	cellular_component	nucleus
K	0005694	cellular_component	chromosome
K	0005739	cellular_component	mitochondrion
K	0006338	biological_process	chromatin remodeling
K	0006357	biological_process	regulation of transcription by RNA polymerase II
K	0007059	biological_process	chromosome segregation
K	0019237	molecular_function	centromeric DNA binding
K	0042762	biological_process	regulation of sulfur metabolic process
K	0045944	biological_process	positive regulation of transcription by RNA polymerase II
K	0046983	molecular_function	protein dimerization activity
K	0061431	biological_process	cellular response to methionine
K	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
K	0089713	cellular_component	Cbf1-Met4-Met28 complex
K	1900059	biological_process	positive regulation of sulfate assimilation
K	1900060	biological_process	negative regulation of ceramide biosynthetic process
K	1900090	biological_process	positive regulation of inositol biosynthetic process
L	0000122	biological_process	negative regulation of transcription by RNA polymerase II
L	0000775	cellular_component	chromosome, centromeric region
L	0000776	cellular_component	kinetochore
L	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
L	0001227	molecular_function	DNA-binding transcription repressor activity, RNA polymerase II-specific
L	0001228	molecular_function	DNA-binding transcription activator activity, RNA polymerase II-specific
L	0003677	molecular_function	DNA binding
L	0003700	molecular_function	DNA-binding transcription factor activity
L	0005634	cellular_component	nucleus
L	0005694	cellular_component	chromosome
L	0005739	cellular_component	mitochondrion
L	0006338	biological_process	chromatin remodeling
L	0006357	biological_process	regulation of transcription by RNA polymerase II
L	0007059	biological_process	chromosome segregation
L	0019237	molecular_function	centromeric DNA binding
L	0042762	biological_process	regulation of sulfur metabolic process
L	0045944	biological_process	positive regulation of transcription by RNA polymerase II
L	0046983	molecular_function	protein dimerization activity
L	0061431	biological_process	cellular response to methionine
L	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
L	0089713	cellular_component	Cbf1-Met4-Met28 complex
L	1900059	biological_process	positive regulation of sulfate assimilation
L	1900060	biological_process	negative regulation of ceramide biosynthetic process
L	1900090	biological_process	positive regulation of inositol biosynthetic process

Functional Information from PROSITE/UniProt

site_id	PS00014
Number of Residues	4
Details	ER_TARGET Endoplasmic reticulum targeting sequence. SQEL

Chain	Residue	Details
C	SER128-LEU131

site_id	PS00046
Number of Residues	7
Details	HISTONE_H2A Histone H2A signature. AGLtFPV

Chain	Residue	Details
C	ALA22-VAL28

site_id	PS00047
Number of Residues	5
Details	HISTONE_H4 Histone H4 signature. GAKRH

Chain	Residue	Details
B	GLY14-HIS18

site_id	PS00322
Number of Residues	7
Details	HISTONE_H3_1 Histone H3 signature 1. KAPRKQL

Chain	Residue	Details
A	LYS14-LEU20

site_id	PS00357
Number of Residues	23
Details	HISTONE_H2B Histone H2B signature. REIQTavRlILpGELaKHAVSEG

Chain	Residue	Details
D	ARG95-GLY117

site_id	PS00959
Number of Residues	9
Details	HISTONE_H3_2 Histone H3 signature 2. PFqRLVREI

Chain	Residue	Details
A	PRO66-ILE74

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphoserine; by ATM or ATR => ECO:0007744\|PubMed:18407956, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
K	SER45
L	SER45
H	LYS6
H	LYS7

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18407956, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
K	SER48
L	SER48

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
K	SER84
L	SER84

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17330950, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
K	THR138
L	THR138

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0000269\|PubMed:19113941

Chain	Residue	Details
D	LYS17
H	LYS17
G	LYS13
G	LYS21
F	LYS8
F	LYS16
F	LYS44
F	LYS79

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: N6-butyryllysine; alternate => ECO:0000269\|PubMed:19113941

Chain	Residue	Details
D	LYS21
H	LYS21
F	LYS12
F	LYS20

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: N6-methyllysine; alternate => ECO:0000269\|PubMed:19113941

Chain	Residue	Details
D	LYS22
H	LYS22
F	LYS31
F	LYS91

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: N6-succinyllysine => ECO:0000269\|PubMed:22389435

Chain	Residue	Details
D	LYS34
D	LYS46
H	LYS34
H	LYS46

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: N6,N6-dimethyllysine => ECO:0000269\|PubMed:19113941

Chain	Residue	Details
D	LYS37
H	LYS37
G	LYS126
F	TYR88

site_id	SWS_FT_FI10
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate

Chain	Residue	Details
D	LYS6
E	LYS64
D	LYS7
H	LYS6
H	LYS7
A	LYS64
E	LYS18
E	LYS23
E	LYS27
E	LYS36

site_id	SWS_FT_FI11
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:10642555, ECO:0000269\|PubMed:12535538, ECO:0000269\|PubMed:12535539, ECO:0000269\|PubMed:14660635, ECO:0000269\|PubMed:15280549, ECO:0000269\|PubMed:16432255

Chain	Residue	Details
B	LYS77
D	LYS123
H	LYS123

site_id	SWS_FT_FI12
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate => ECO:0000305

Chain	Residue	Details
D	LYS16
D	LYS17
H	LYS16
H	LYS17

site_id	SWS_FT_FI13
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250\|UniProtKB:P62805

Chain	Residue	Details
A	LYS37
B	LYS91
F	LYS91

site_id	SWS_FT_FI14
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250\|UniProtKB:Q71DI3

Chain	Residue	Details
A	TYR41
E	TYR41

site_id	SWS_FT_FI15
Number of Residues	4
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:P84228

Chain	Residue	Details
A	LYS56
A	LYS79
E	LYS56
E	LYS79

site_id	SWS_FT_FI16
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q71DI3

Chain	Residue	Details
A	SER57
E	SER57

site_id	SWS_FT_FI17
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0000250\|UniProtKB:Q71DI3

Chain	Residue	Details
A	THR80
A	THR107
E	THR80
E	THR107

site_id	SWS_FT_FI18
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P84243

Chain	Residue	Details
A	SER86
E	SER86

site_id	SWS_FT_FI19
Number of Residues	2
Details	MOD_RES: N6-glutaryllysine; alternate => ECO:0000250\|UniProtKB:Q71DI3

Chain	Residue	Details
A	LYS115
E	LYS115

site_id	SWS_FT_FI20
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:Q71DI3

Chain	Residue	Details
A	LYS122
E	LYS122

site_id	SWS_FT_FI21
Number of Residues	2
Details	LIPID: S-palmitoyl cysteine => ECO:0000250\|UniProtKB:Q71DI3

Chain	Residue	Details
A	CYS110
E	CYS110

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)