7SQ9

Cryo-EM structure of mouse temsirolimus/PI(3,5)P2-bound TRPML1 channel at 2.11 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Mucolipin-1	polymer	580	65573.6	4	UniProt (Q99J21) Pfam (PF21381) Pfam (PF08016)	Mus musculus (house mouse)	Mucolipidin,Transient receptor potential-mucolipin 1,TRPML1
2	E, F, G, H (E, F, G, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
3	I, M, O, Q (A, B, C, D)	(2R)-3-{[(S)-hydroxy{[(1S,2R,3R,4S,5S,6R)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dioctanoate	non-polymer		746.6	4	Chemie (EUJ) PubChem (53394548) PubChem (177449291) PubChem (11657962) PubChem (99647643) PubChem (99647642) PubChem (136212532)
4	J, N, P, R (A, B, C, D)	(1R,2R,4S)-4-{(2R)-2-[(3S,6R,7E,9R,10R,12R,14S,15E,17E,19E,21S,23S,26R,27R,30S,34aS)-9,27-dihydroxy-10,21-dimethoxy-6,8,12,14,20,26-hexamethyl-1,5,11,28,29-pentaoxo-1,4,5,6,9,10,11,12,13,14,21,22,23,24,25,26,27,28,29,31,32,33,34,34a-tetracosahydro-3H-23,27-epoxypyrido[2,1-c][1,4]oxazacyclohentriacontin-3-yl]propyl}-2-methoxycyclohexyl 3-hydroxy-2-(hydroxymethyl)-2-methylpropanoate	non-polymer		1030.3	4	Chemie (A4I) PubChem (22525119) PubChem (22829018) PubChem (23308413) PubChem (24847874) PubChem (18293306) PubChem (20679390) PubChem (23305221) PubChem (23375915) PubChem (23724530) PubChem (25200276) PubChem (44476486) PubChem (53486199) PubChem (54608508) PubChem (56845670) PubChem (57968260) PubChem (58225486) PubChem (59756828) PubChem (59965665) PubChem (71296107) PubChem (71752350) PubChem (177675638) PubChem (73265234) PubChem (73755165) PubChem (86287409) PubChem (89569100) PubChem (148191) PubChem (6918289) PubChem (388784) PubChem (71312019) PubChem (71433778) PubChem (71772382) PubChem (73440822) PubChem (73896897) PubChem (73945186) PubChem (86277830) PubChem (87159445) PubChem (88568175) PubChem (90069184) PubChem (5938194) PubChem (118133659) PubChem (6440033) PubChem (122361634) PubChem (122361642) PubChem (122361737) PubChem (122580978) PubChem (9876533) PubChem (129009966) PubChem (129010328) PubChem (129197491) PubChem (129317846) PubChem (132274070) PubChem (132939739) PubChem (121605801) PubChem (134812825) PubChem (122361790) PubChem (122361806) PubChem (122361808) PubChem (122361839) PubChem (122361902) PubChem (122361911) PubChem (122362113) PubChem (123745191) PubChem (138404327) PubChem (140581569) PubChem (140963686) PubChem (146569567) PubChem (147191959) PubChem (152772378) PubChem (154825565) PubChem (135391151) PubChem (158164882) PubChem (157529408) PubChem (160235249) PubChem (137295275) PubChem (163285206) PubChem (163359784) PubChem (163477371) PubChem (163544722) PubChem (138106907) PubChem (138991580) PubChem (166639522) PubChem (168012331) PubChem (168317584) PubChem (168313165) PubChem (144053478) PubChem (145712265) PubChem (154609062) PubChem (171336787) PubChem (171470476) PubChem (155809408) PubChem (172503255) PubChem (157529409) PubChem (157529410) PubChem (157529411) PubChem (159585421) PubChem (173500348) PubChem (173586428) PubChem (173650339) PubChem (173557031) PubChem (174069408) PubChem (176447814) PubChem (163477372) PubChem (168313304) PubChem (168315396) PubChem (171159773) PubChem (171689466) PubChem (172897884) PubChem (173500621) PubChem (173586429) PubChem (174069405) PubChem (174069406)
5	K, L (A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA) PubChem (923)
6	S, T, U, V (A, B, C, D)	water	water		18.0	16	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	262294.5
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	10
	Total formula weight	7153.4
All*	Total formula weight	271145.5

*Water molecules are not included.