7SQ2

Reprocessed and refined structure of Phospholipase C-beta and Gq signaling complex

Replaces:  3OHM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(q) subunit alpha	polymer	327	38256.4	1	UniProt (P21279) Pfam (PF00503)	Mus musculus (Mouse)	Guanine nucleotide-binding protein alpha-q
2	B (B)	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3	polymer	885	99865.3	1	UniProt (Q01970) Pfam (PF17787) Pfam (PF22631) Pfam (PF00388) Pfam (PF00387) Pfam (PF00168)	Homo sapiens (Human)	Phosphoinositide phospholipase C-beta-3,Phospholipase C-beta-3,PLC-beta-3
3	C (A)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	1	Chemie (ALF)
4	D, N (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	E (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
6	F, G, H, I, J... (A, B)	ACETATE ION	non-polymer		59.0	7	Chemie (ACT)
7	M (B)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
8	O, P (A, B)	water	water		18.0	215	Chemie (HOH)

Sequence modifications

A: 35 - 359 (UniProt: P21279)

PDB	External Database	Details
Gly 33	-	expression tag
Ala 34	-	expression tag

B: 10 - 886 (UniProt: Q01970)

PDB	External Database	Details
Gly 2	-	expression tag
Ala 3	-	expression tag
Met 4	-	expression tag
Asp 5	-	expression tag
Pro 6	-	expression tag
Glu 7	-	expression tag
Phe 8	-	expression tag
Met 9	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	138121.7
Non-Polymers*	Number of molecules	12
	Total formula weight	1048.2
All*	Total formula weight	139169.9

*Water molecules are not included.