7SQ2
Reprocessed and refined structure of Phospholipase C-beta and Gq signaling complex
Replaces: 3OHMExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 202.837, 90.817, 93.135 |
Unit cell angles | 90.00, 101.19, 90.00 |
Refinement procedure
Resolution | 40.550 - 2.600 |
R-factor | 0.20247 |
Rwork | 0.199 |
R-free | 0.26334 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3OHM |
RMSD bond length | 0.003 |
RMSD bond angle | 1.227 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.550 | 2.666 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmeas | 0.084 | 0.723 |
Rpim | 0.035 | 0.304 |
Number of reflections | 48454 | 2555 |
<I/σ(I)> | 32.5 | 2.67 |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 5.6 | 5.5 |
CC(1/2) | 0.905 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 7% PEG 6000, 100 mM Hepes, 1 mM CaCl2, 100 mM magnesium acetate |