7SD0

Cryo-EM structure of the SHOC2:PP1C:MRAS complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Leucine-rich repeat protein SHOC-2	polymer	585	65157.0	1	UniProt (Q9UQ13) Pfam (PF13855) In PDB	Homo sapiens (Human)	Protein soc-2 homolog,Protein sur-8 homolog
2	B	Ras-related protein M-Ras	polymer	210	24028.6	1	UniProt (O14807) Pfam (PF00071) In PDB	Homo sapiens (Human)	Ras-related protein R-Ras3
3	C	Serine/threonine-protein phosphatase PP1-gamma catalytic subunit	polymer	325	37174.9	1	UniProt (P36873) Pfam (PF16891) Pfam (PF00149) In PDB	Homo sapiens (Human)	PP-1G,Protein phosphatase 1C catalytic subunit
4	B	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	1	Chemie (GCP)
5	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	C	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)

Sequence modifications

A: 2 - 582 (UniProt: Q9UQ13)

PDB	External Database	Details
Gly 1	-	expression tag
Gly 583	-	expression tag
Asn 584	-	expression tag
Ser 585	-	expression tag

B: 1 - 208 (UniProt: O14807)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

C: 1 - 323 (UniProt: P36873)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	126360.5
Non-Polymers*	Number of molecules	4
	Total formula weight	655.4
All*	Total formula weight	127015.9

*Water molecules are not included.