7S7B

Human Nuclear exosome targeting (NEXT) complex homodimer bound to RNA (substrate 1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Exosome RNA helicase MTR4	polymer	1045	118225.0	2	UniProt (P42285) Pfam (PF00270) Pfam (PF00271) Pfam (PF21408) Pfam (PF13234) Pfam (PF08148) In PDB	Homo sapiens (Human)	MTR4, MTREX,ATP-dependent RNA helicase DOB1,ATP-dependent RNA helicase SKIV2L2,Superkiller viralicidic activity 2-like 2,TRAMP-like complex helicase
2	B, F	Zinc finger CCHC domain-containing protein 8,Zinc finger CCHC domain-containing protein 8	polymer	618	69259.8	2	UniProt (Q6NZY4) Pfam (PF00098) Pfam (PF04046) In PDB	Homo sapiens (Human)	TRAMP-like complex RNA-binding factor ZCCHC8,TRAMP-like complex RNA-binding factor ZCCHC8
3	C, G	RNA-binding protein 7	polymer	83	9463.0	2	UniProt (Q9Y580) Pfam (PF00076) In PDB	Homo sapiens (Human)	RNA-binding motif protein 7
4	D, H	RNA (46-MER)	polymer	46	14605.8	2		synthetic construct
5	B, F	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, E: 1 - 1042 (UniProt: P42285)

PDB	External Database	Details
Ser -2	-	expression tag
Gly -1	-	expression tag
Asp 0	-	expression tag

B, F: 1 - 507 (UniProt: Q6NZY4)

PDB	External Database	Details
Ser -2	-	expression tag
Gly -1	-	expression tag
Asp 0	-	expression tag

B, F: 508 - 707 (UniProt: Q6NZY4)
C, G: 7 - 86 (UniProt: Q9Y580)

PDB	External Database	Details
Ser 4	-	expression tag
Gly 5	-	expression tag
Asp 6	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	423107.1
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	423238.0

*Water molecules are not included.