7S6D

CryoEM structure of modular PKS holo-Lsd14 bound to antibody fragment 1B2, composite structure

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, A, B	6-deoxyerythronolide-B synthase EryA2, modules 3 and 4, Lsd14 Polyketide synthase fusion	polymer	1649	174085.7	3	UniProt (Q03132) UniProt (B6ZK67) Pfam (PF08990) Pfam (PF00109) Pfam (PF02801) Pfam (PF16197) Pfam (PF00698) Pfam (PF18369) Pfam (PF08659) Pfam (PF00550) In PDB	Saccharopolyspora erythraea	DEBS 2,6-deoxyerythronolide B synthase II,Erythronolide synthase,ORF B
2	E, D	Fab 1B2 heavy chain	polymer	249	26447.6	2		Homo sapiens (Human)
3	G, F	Fab 1B2 light chain	polymer	236	25715.8	2		Homo sapiens (Human)
4	C	2'-MONOPHOSPHOADENOSINE-5'-DIPHOSPHATE	non-polymer		507.2	1	Chemie (ATR)

Sequence modifications

C, A, B: 9 - 37 (UniProt: Q03132)

PDB	External Database	Details
Met 7	-	initiating methionine
Val 8	-	expression tag

C, A, B: 38 - 1647 (UniProt: B6ZK67)

PDB	External Database	Details
Leu 1648	-	expression tag
Glu 1649	-	expression tag
His 1650	-	expression tag
His 1651	-	expression tag
His 1652	-	expression tag
His 1653	-	expression tag
His 1654	-	expression tag
His 1655	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	626584.1
Non-Polymers*	Number of molecules	1
	Total formula weight	507.2
All*	Total formula weight	627091.3

*Water molecules are not included.