7S6D
CryoEM structure of modular PKS holo-Lsd14 bound to antibody fragment 1B2, composite structure
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.1 |
Number of Particle | 295592 |
Software | cryoSPARC,PHENIX,RELION,EPU,Coot |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |