7RHQ

Cryo-EM structure of Xenopus Patched-1 in complex with GAS1 and Sonic Hedgehog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Patched-1	polymer	1205	134622.4	1	UniProt (Q98SW6) Pfam (PF12349) Pfam (PF02460) In PDB	Xenopus laevis (African clawed frog)
2	C	Sonic hedgehog protein N-product	polymer	192	21378.9	1	UniProt (Q15465) Pfam (PF01085) In PDB	Homo sapiens (human)	ShhN,Shh N-terminal processed signaling domains,ShhNp
3	G	Growth arrest-specific protein 1	polymer	282	29546.8	1	UniProt (P54826) Pfam (PF02351) In PDB	Homo sapiens (human)	GAS-1
4	B, D, E	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
5	A, C	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
6	A	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	1	Chemie (Y01)
7	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
8	C	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
9	C	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
10	C	PALMITIC ACID	non-polymer		256.4	1	Chemie (PLM)

Sequence modifications

A: 1 - 1178 (UniProt: Q98SW6)

PDB	External Database	Details
Ser 1179	-	expression tag
Ser 1180	-	expression tag
Ser 1181	-	expression tag
Pro 1182	-	expression tag
Ala 1183	-	expression tag
Ala 1184	-	expression tag
Ala 1185	-	expression tag
His 1186	-	expression tag
His 1187	-	expression tag
His 1188	-	expression tag
His 1189	-	expression tag
His 1190	-	expression tag
His 1191	-	expression tag
His 1192	-	expression tag
His 1193	-	expression tag
Glu 1194	-	expression tag
Asp 1195	-	expression tag
Gln 1196	-	expression tag
Val 1197	-	expression tag
Asp 1198	-	expression tag
Pro 1199	-	expression tag
Arg 1200	-	expression tag
Leu 1201	-	expression tag
Ile 1202	-	expression tag
Asp 1203	-	expression tag
Gly 1204	-	expression tag
Lys 1205	-	expression tag

C: 24 - 197 (UniProt: Q15465)

PDB	External Database	Details
Gly 130	-	insertion
Gly 130	-	insertion
Thr 130	-	insertion
Glu 130	-	insertion
Asp 130	-	insertion
Gln 130	-	insertion
Val 130	-	insertion
Asp 130	-	insertion
Pro 130	-	insertion
Arg 130	-	insertion
Leu 130	-	insertion
Ile 130	-	insertion
Asp 130	-	insertion
Gly 130	-	insertion
Lys 130	-	insertion
Ser 130	-	insertion
Gly 130	-	insertion
Gly 130	-	insertion

G: 40 - 317 (UniProt: P54826)

PDB	External Database	Details
Gly 36	-	expression tag
Pro 37	-	expression tag
Gly 38	-	expression tag
Ser 39	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	185548.1
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	9
	Total formula weight	2104.4
All*	Total formula weight	188925.7

*Water molecules are not included.