7RHQ
Cryo-EM structure of Xenopus Patched-1 in complex with GAS1 and Sonic Hedgehog
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Patched-1 | polymer | 1205 | 134622.4 | 1 | UniProt (Q98SW6) Pfam (PF12349) | Xenopus laevis (African clawed frog) | |
| 2 | B (C) | Sonic hedgehog protein N-product | polymer | 192 | 21378.9 | 1 | UniProt (Q15465) Pfam (PF01085) | Homo sapiens (human) | ShhN,Shh N-terminal processed signaling domains,ShhNp |
| 3 | C (G) | Growth arrest-specific protein 1 | polymer | 282 | 29546.8 | 1 | UniProt (P54826) Pfam (PF02351) | Homo sapiens (human) | GAS-1 |
| 4 | D, E, F (B, D, E) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 5 | G, K (A, C) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
| 6 | H (A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 1 | Chemie (Y01) | |||
| 7 | I, J (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 8 | L (C) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 9 | M, N (C) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 10 | O (C) | PALMITIC ACID | non-polymer | 256.4 | 1 | Chemie (PLM) |
Sequence modifications
A: 1 - 1178 (UniProt: Q98SW6)
C: 24 - 197 (UniProt: Q15465)
G: 40 - 317 (UniProt: P54826)
| PDB | External Database | Details |
|---|---|---|
| Ser 1179 | - | expression tag |
| Ser 1180 | - | expression tag |
| Ser 1181 | - | expression tag |
| Pro 1182 | - | expression tag |
| Ala 1183 | - | expression tag |
| Ala 1184 | - | expression tag |
| Ala 1185 | - | expression tag |
| His 1186 | - | expression tag |
| His 1187 | - | expression tag |
| His 1188 | - | expression tag |
| His 1189 | - | expression tag |
| His 1190 | - | expression tag |
| His 1191 | - | expression tag |
| His 1192 | - | expression tag |
| His 1193 | - | expression tag |
| Glu 1194 | - | expression tag |
| Asp 1195 | - | expression tag |
| Gln 1196 | - | expression tag |
| Val 1197 | - | expression tag |
| Asp 1198 | - | expression tag |
| Pro 1199 | - | expression tag |
| Arg 1200 | - | expression tag |
| Leu 1201 | - | expression tag |
| Ile 1202 | - | expression tag |
| Asp 1203 | - | expression tag |
| Gly 1204 | - | expression tag |
| Lys 1205 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 130 | - | insertion |
| Gly 130 | - | insertion |
| Thr 130 | - | insertion |
| Glu 130 | - | insertion |
| Asp 130 | - | insertion |
| Gln 130 | - | insertion |
| Val 130 | - | insertion |
| Asp 130 | - | insertion |
| Pro 130 | - | insertion |
| Arg 130 | - | insertion |
| Leu 130 | - | insertion |
| Ile 130 | - | insertion |
| Asp 130 | - | insertion |
| Gly 130 | - | insertion |
| Lys 130 | - | insertion |
| Ser 130 | - | insertion |
| Gly 130 | - | insertion |
| Gly 130 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Gly 36 | - | expression tag |
| Pro 37 | - | expression tag |
| Gly 38 | - | expression tag |
| Ser 39 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 185548.1 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1273.2 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2104.4 | |
| All* | Total formula weight | 188925.7 |
