7RE3

SARS-CoV-2 replication-transcription complex bound to nsp13 helicase - nsp13(2)-RTC dimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, G	RNA-directed RNA polymerase	polymer	932	106781.0	2	UniProt (P0DTD1) Pfam (PF06478) Pfam (PF00680) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	Pol, RdRp, Non-structural protein 12, nsp12
2	B, D, H, J	Non-structural protein 8	polymer	199	22034.2	4	UniProt (P0DTD1) Pfam (PF08717) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	nsp8
3	C, I	Non-structural protein 7	polymer	88	9748.4	2	UniProt (P0DTD1) Pfam (PF08716) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	nsp7
4	E, F, K, L	Helicase	polymer	605	67354.0	4	UniProt (P0DTD1) Pfam (PF20632) Pfam (PF20633) Pfam (PF20631) Pfam (PF13087) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	Hel, Non-structural protein 13, nsp13
5	P, Q	Product RNA	polymer	35	11141.6	2		synthetic construct
6	T, U	Template RNA	polymer	55	17573.4	2		synthetic construct
7	F, G, K, A, L...	ZINC ION	non-polymer		65.4	16	Chemie (ZN)
8	F, G, K, A, L...	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
9	F, G, K, A, L...	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	6	Chemie (ADP)
10	F, K, L, E	ALUMINUM FLUORIDE	non-polymer		84.0	4	Chemie (AF3)
11	E, K	CHAPSO	non-polymer		631.9	2	Chemie (1N7)

Sequence modifications

B, D, H, J: 1 - 198 (UniProt: P0DTD1)

PDB	External Database	Details
Met 0	-	initiating methionine

C, I: 1 - 83 (UniProt: P0DTD1)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Val -2	-	expression tag
Asp -1	-	expression tag
Met 0	-	expression tag

E, F, K, L: 1 - 601 (UniProt: P0DTD1)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	648041.9
Non-Polymers*	Number of molecules	34
	Total formula weight	5355.3
All*	Total formula weight	653397.2

*Water molecules are not included.