7QQB

Crystal structure of the envelope glycoprotein complex of Puumala virus in complex with the scFv fragment of the broadly neutralizing human antibody ADI-42898

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Envelope polyprotein	polymer	889	95672.6	2	UniProt (A0A0B4U5I0) UniProt (A0A6M3W7M6) Pfam (PF01567) Pfam (PF01561) Pfam (PF20682)	Puumala orthohantavirus	M polyprotein
2	C, D (H, L)	Single Chain Variable Fragment (scFv) of ADI-42898	polymer	298	31558.8	2		Homo sapiens
3	E (C)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G56014GC)
4	F, G (D, E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
5	H (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	I, J, K, L (A, B, H, L)	water	water		18.0	26	Chemie (HOH)

Sequence modifications

A, B: 20 - 381 (UniProt: A0A0B4U5I0)

PDB	External Database	Details
Arg 18	-	expression tag
Ser 19	-	expression tag
Asp 362	Glu 362	conflict
Gly 382	-	linker
Gly 383	-	linker
Ser 384	-	linker
Gly 385	-	linker
Leu 386	-	linker
Val 387	-	linker
Pro 388	-	linker
Arg 389	-	linker
Gly 390	-	linker
Ser 391	-	linker
Gly 392	-	linker
Gly 393	-	linker
Gly 394	-	linker
Ser 395	-	linker
Gly 396	-	linker
Gly 397	-	linker
Gly 398	-	linker
Ser 399	-	linker
Trp 400	-	linker
Ser 401	-	linker
His 402	-	linker
Pro 403	-	linker
Gln 404	-	linker
Phe 405	-	linker
Glu 406	-	linker
Lys 407	-	linker
Gly 408	-	linker
Gly 409	-	linker
Gly 410	-	linker
Thr 411	-	linker
Gly 412	-	linker
Gly 413	-	linker
Gly 414	-	linker
Thr 415	-	linker
Leu 416	-	linker
Val 417	-	linker
Pro 418	-	linker
Arg 419	-	linker
Gly 420	-	linker
Ser 421	-	linker
Gly 422	-	linker
Thr 423	-	linker
Gly 424	-	linker
Gly 425	-	linker

A, B: 426 - 860 (UniProt: A0A6M3W7M6)

PDB	External Database	Details
Gly 861	-	expression tag
Leu 862	-	expression tag
Val 863	-	expression tag
Pro 864	-	expression tag
Arg 865	-	expression tag
Gly 866	-	expression tag
Ser 867	-	expression tag
Gly 868	-	expression tag
Pro 869	-	expression tag
Phe 870	-	expression tag
Glu 871	-	expression tag
Asp 872	-	expression tag
Asp 873	-	expression tag
Asp 874	-	expression tag
Asp 875	-	expression tag
Lys 876	-	expression tag
Ala 877	-	expression tag
Gly 878	-	expression tag
Trp 879	-	expression tag
Ser 880	-	expression tag
His 881	-	expression tag
Pro 882	-	expression tag
Gln 883	-	expression tag
Phe 884	-	expression tag
Glu 885	-	expression tag
Lys 886	-	expression tag
Gly 887	-	expression tag
Gly 888	-	expression tag
Gly 889	-	expression tag
Ser 890	-	expression tag
Gly 891	-	expression tag
Gly 892	-	expression tag
Gly 893	-	expression tag
Ser 894	-	expression tag
Gly 895	-	expression tag
Gly 896	-	expression tag
Gly 897	-	expression tag
Ser 898	-	expression tag
Trp 899	-	expression tag
Ser 900	-	expression tag
His 901	-	expression tag
Pro 902	-	expression tag
Gln 903	-	expression tag
Phe 904	-	expression tag
Glu 905	-	expression tag
Lys 906	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	254462.8
Branched	Number of molecules	3
	Total formula weight	1921.8
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	256476.7

*Water molecules are not included.