7QQB

Crystal structure of the envelope glycoprotein complex of Puumala virus in complex with the scFv fragment of the broadly neutralizing human antibody ADI-42898

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	83.876, 109.304, 149.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.000 - 2.600
Rwork	0.212
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y5f
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.890	39.890	2.700
High resolution limit [Å]	2.600	9.730	2.600
Rmerge	0.120	0.029	2.912
Rmeas	0.126	0.030	3.043
Rpim	0.037	0.010	0.879
Total number of observations	495751	8957	53168
Number of reflections	43057	925	4457
<I/σ(I)>	14.2	57.4	0.9
Completeness [%]	100.0	98.7	100
Redundancy	11.5	9.7	11.9
CC(1/2)	0.999	0.999	0.503

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	25%(w/v) PEG 1000, 0.1M Hepes 7.5