7QNB
Cryo-EM structure of human full-length beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Gamma-aminobutyric acid type A receptor subunit gamma2 | polymer | 487 | 55666.8 | 2 | UniProt (A0A2K5TLN2) Pfam (PF02931) Pfam (PF02932) UniProt (by SIFTS) (P18507) | Homo sapiens (human) | |
| 2 | B, D, E (B, D, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54180.3 | 3 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3 |
| 3 | F (N) | Nanobody Nb25 | polymer | 121 | 13341.7 | 1 | Lama glama | ||
| 4 | G, I, K (b, d, e) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 5 | H, J, L (F, G, H) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G22768VO) | |||
| 6 | M, O (A, C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 7 | N (B) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 1 | Chemie (ABU) |
Sequence modifications
A, C: 12 - 436 (UniProt: A0A2K5TLN2)
| PDB | External Database | Details |
|---|---|---|
| Met -30 | - | initiating methionine |
| Gly -29 | - | expression tag |
| Ile -28 | - | expression tag |
| Leu -27 | - | expression tag |
| Pro -26 | - | expression tag |
| Ser -25 | - | expression tag |
| Pro -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Met -22 | - | expression tag |
| Pro -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Leu -19 | - | expression tag |
| Leu -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Leu -16 | - | expression tag |
| Val -15 | - | expression tag |
| Ser -14 | - | expression tag |
| Leu -13 | - | expression tag |
| Leu -12 | - | expression tag |
| Ser -11 | - | expression tag |
| Val -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Met -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Cys -5 | - | expression tag |
| Val -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Glu -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Asp 4 | - | expression tag |
| Asp 5 | - | expression tag |
| Asp 6 | - | expression tag |
| Tyr 7 | - | expression tag |
| Glu 8 | - | expression tag |
| Asp 9 | - | expression tag |
| Tyr 10 | - | expression tag |
| Thr 11 | - | expression tag |
| Gly 437 | - | expression tag |
| Thr 438 | - | expression tag |
| Gly 439 | - | expression tag |
| Gly 440 | - | expression tag |
| Ser 441 | - | expression tag |
| Gly 442 | - | expression tag |
| Gly 443 | - | expression tag |
| Ser 444 | - | expression tag |
| Gly 445 | - | expression tag |
| Gly 446 | - | expression tag |
| Ser 447 | - | expression tag |
| Thr 448 | - | expression tag |
| Glu 449 | - | expression tag |
| Thr 450 | - | expression tag |
| Ser 451 | - | expression tag |
| Gln 452 | - | expression tag |
| Val 453 | - | expression tag |
| Ala 454 | - | expression tag |
| Pro 455 | - | expression tag |
| Ala 456 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 287216.4 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 4005.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 545.5 | |
| All* | Total formula weight | 291767.6 |
