Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7QNB

Cryo-EM structure of human full-length beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004888	molecular_function	transmembrane signaling receptor activity
A	0004890	molecular_function	GABA-A receptor activity
A	0005216	molecular_function	monoatomic ion channel activity
A	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
A	0005254	molecular_function	chloride channel activity
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0006811	biological_process	monoatomic ion transport
A	0006821	biological_process	chloride transport
A	0007214	biological_process	gamma-aminobutyric acid signaling pathway
A	0007268	biological_process	chemical synaptic transmission
A	0008503	molecular_function	benzodiazepine receptor activity
A	0009791	biological_process	post-embryonic development
A	0016020	cellular_component	membrane
A	0022851	molecular_function	GABA-gated chloride ion channel activity
A	0030424	cellular_component	axon
A	0030425	cellular_component	dendrite
A	0030534	biological_process	adult behavior
A	0030594	molecular_function	neurotransmitter receptor activity
A	0030659	cellular_component	cytoplasmic vesicle membrane
A	0031410	cellular_component	cytoplasmic vesicle
A	0032590	cellular_component	dendrite membrane
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0034707	cellular_component	chloride channel complex
A	0042995	cellular_component	cell projection
A	0043005	cellular_component	neuron projection
A	0045202	cellular_component	synapse
A	0045211	cellular_component	postsynaptic membrane
A	0051932	biological_process	synaptic transmission, GABAergic
A	0060078	biological_process	regulation of postsynaptic membrane potential
A	0071420	biological_process	cellular response to histamine
A	0098794	cellular_component	postsynapse
A	0098982	cellular_component	GABA-ergic synapse
A	0099634	cellular_component	postsynaptic specialization membrane
A	1902476	biological_process	chloride transmembrane transport
A	1902495	cellular_component	transmembrane transporter complex
A	1902711	cellular_component	GABA-A receptor complex
A	1904315	molecular_function	transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential
A	1904862	biological_process	inhibitory synapse assembly
B	0004888	molecular_function	transmembrane signaling receptor activity
B	0004890	molecular_function	GABA-A receptor activity
B	0005216	molecular_function	monoatomic ion channel activity
B	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
B	0006811	biological_process	monoatomic ion transport
B	0016020	cellular_component	membrane
B	0034220	biological_process	monoatomic ion transmembrane transport
C	0004888	molecular_function	transmembrane signaling receptor activity
C	0004890	molecular_function	GABA-A receptor activity
C	0005216	molecular_function	monoatomic ion channel activity
C	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
C	0005254	molecular_function	chloride channel activity
C	0005515	molecular_function	protein binding
C	0005886	cellular_component	plasma membrane
C	0006811	biological_process	monoatomic ion transport
C	0006821	biological_process	chloride transport
C	0007214	biological_process	gamma-aminobutyric acid signaling pathway
C	0007268	biological_process	chemical synaptic transmission
C	0008503	molecular_function	benzodiazepine receptor activity
C	0009791	biological_process	post-embryonic development
C	0016020	cellular_component	membrane
C	0022851	molecular_function	GABA-gated chloride ion channel activity
C	0030424	cellular_component	axon
C	0030425	cellular_component	dendrite
C	0030534	biological_process	adult behavior
C	0030594	molecular_function	neurotransmitter receptor activity
C	0030659	cellular_component	cytoplasmic vesicle membrane
C	0031410	cellular_component	cytoplasmic vesicle
C	0032590	cellular_component	dendrite membrane
C	0034220	biological_process	monoatomic ion transmembrane transport
C	0034707	cellular_component	chloride channel complex
C	0042995	cellular_component	cell projection
C	0043005	cellular_component	neuron projection
C	0045202	cellular_component	synapse
C	0045211	cellular_component	postsynaptic membrane
C	0051932	biological_process	synaptic transmission, GABAergic
C	0060078	biological_process	regulation of postsynaptic membrane potential
C	0071420	biological_process	cellular response to histamine
C	0098794	cellular_component	postsynapse
C	0098982	cellular_component	GABA-ergic synapse
C	0099634	cellular_component	postsynaptic specialization membrane
C	1902476	biological_process	chloride transmembrane transport
C	1902495	cellular_component	transmembrane transporter complex
C	1902711	cellular_component	GABA-A receptor complex
C	1904315	molecular_function	transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential
C	1904862	biological_process	inhibitory synapse assembly
D	0004888	molecular_function	transmembrane signaling receptor activity
D	0004890	molecular_function	GABA-A receptor activity
D	0005216	molecular_function	monoatomic ion channel activity
D	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
D	0006811	biological_process	monoatomic ion transport
D	0016020	cellular_component	membrane
D	0034220	biological_process	monoatomic ion transmembrane transport
E	0004888	molecular_function	transmembrane signaling receptor activity
E	0004890	molecular_function	GABA-A receptor activity
E	0005216	molecular_function	monoatomic ion channel activity
E	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
E	0006811	biological_process	monoatomic ion transport
E	0016020	cellular_component	membrane
E	0034220	biological_process	monoatomic ion transmembrane transport

Functional Information from PROSITE/UniProt

site_id	PS00236
Number of Residues	15
Details	NEUROTR_ION_CHANNEL Neurotransmitter-gated ion-channels signature. CmMdLrrYPlDeqnC

Chain	Residue	Details
B	CYS136-CYS150
A	CYS151-CYS165

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	166
Details	TRANSMEM: Helical => ECO:0000269\|PubMed:30602789, ECO:0007744\|PDB:6I53

Chain	Residue	Details
A	THR237-ILE257
A	ALA264-ALA283
A	MET296-PHE320
A	TYR413-TYR433
C	THR237-ILE257
C	ALA264-ALA283
C	MET296-PHE320
C	TYR413-TYR433

site_id	SWS_FT_FI2
Number of Residues	192
Details	TOPO_DOM: Cytoplasmic => ECO:0000305

Chain	Residue	Details
A	ASN258-PRO263
E	SER247-HIS267
E	ALA280-ASN303
E	ILE423-TYR445
A	VAL321-SER412
C	ASN258-PRO263
C	VAL321-SER412
D	ILE222-ILE242
D	SER247-HIS267
D	ALA280-ASN303
D	ILE423-TYR445
E	ILE222-ILE242

site_id	SWS_FT_FI3
Number of Residues	26
Details	TOPO_DOM: Extracellular => ECO:0000305

Chain	Residue	Details
A	ARG284-ALA295
A	LEU434-LEU436
C	ARG284-ALA295
C	LEU434-LEU436
E	ASN243-ALA246
E	TYR304-ALA422

site_id	SWS_FT_FI4
Number of Residues	4
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
A	ASN13
A	ASN90
C	ASN13
C	ASN90

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:29950725, ECO:0000269\|PubMed:30602789, ECO:0007744\|PDB:6D6T, ECO:0007744\|PDB:6I53

Chain	Residue	Details
A	ASN208
C	ASN208
D	ASP95
D	PHE200
E	ASP95
E	PHE200

site_id	SWS_FT_FI6
Number of Residues	9
Details	BINDING: in chain B => ECO:0000269\|PubMed:35355020, ECO:0007744\|PDB:7QN7

Chain	Residue	Details
B	TYR97
B	SER156
B	THR202
D	TYR97
D	SER156
D	THR202
E	TYR97
E	SER156
E	THR202

site_id	SWS_FT_FI7
Number of Residues	3
Details	BINDING: in chain A => ECO:0000269\|PubMed:35355020, ECO:0007744\|PDB:7QN7

Chain	Residue	Details
B	GLU155
D	GLU155
E	GLU155

site_id	SWS_FT_FI8
Number of Residues	3
Details	BINDING: in chain A => ECO:0000269\|PubMed:30140029, ECO:0007744\|PDB:6A96

Chain	Residue	Details
B	TYR157
D	TYR157
E	TYR157

site_id	SWS_FT_FI9
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:24909990, ECO:0000269\|PubMed:35355020, ECO:0007744\|PDB:7QN7

Chain	Residue	Details
B	ASN8
D	ASN8
E	ASN8

site_id	SWS_FT_FI10
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:24909990, ECO:0000269\|PubMed:30602789, ECO:0000269\|PubMed:35355020, ECO:0007744\|PDB:6I53, ECO:0007744\|PDB:7QN7

Chain	Residue	Details
B	ASN80
D	ASN80
E	ASN80

site_id	SWS_FT_FI11
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:24909990, ECO:0000269\|PubMed:30140029, ECO:0000269\|PubMed:30602789, ECO:0000269\|PubMed:35355020, ECO:0007744\|PDB:6A96, ECO:0007744\|PDB:6I53, ECO:0007744\|PDB:7QN7

Chain	Residue	Details
B	ASN149
D	ASN149
E	ASN149

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)