7QNB
Cryo-EM structure of human full-length beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.1 |
Software | PHENIX,RELION,EPU,CTFFIND,Coot,REFMAC |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |