7Q59

Cryo-EM structure of Mycobacterium tuberculosis RNA polymerase holoenzyme dimer comprising sigma factor SigB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, a, b	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	4	UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C, c	DNA-directed RNA polymerase subunit beta	polymer	1174	129602.3	2	UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D, d	DNA-directed RNA polymerase subunit beta'	polymer	1319	147438.3	2	UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E, e	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	2	UniProt (P9WGY5) Pfam (PF01192) In PDB	Mycobacterium tuberculosis H37Rv	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, f	RNA polymerase sigma factor SigB	polymer	343	38572.8	2	UniProt (P9WGI5) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Mycobacterium tuberculosis H37Rv
6	D, d	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
7	D, d	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

C, c: 6 - 1178 (UniProt: P9WGY9)

PDB	External Database	Details
Met 5	-	initiating methionine
Val 6	Ile 6	conflict

D, d: 4 - 1316 (UniProt: P9WGY7)

PDB	External Database	Details
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag

F, f: 1 - 323 (UniProt: P9WGI5)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	805910.5
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	806220.8

*Water molecules are not included.