7Q59

Cryo-EM structure of Mycobacterium tuberculosis RNA polymerase holoenzyme dimer comprising sigma factor SigB

Functional Information from GO Data

Chain	GOid	namespace	contents
a	0000428	cellular_component	DNA-directed RNA polymerase complex
a	0003677	molecular_function	DNA binding
a	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
a	0005737	cellular_component	cytoplasm
a	0005829	cellular_component	cytosol
a	0005886	cellular_component	plasma membrane
a	0006351	biological_process	DNA-templated transcription
a	0009274	cellular_component	peptidoglycan-based cell wall
a	0016779	molecular_function	nucleotidyltransferase activity
a	0034062	molecular_function	5'-3' RNA polymerase activity
a	0046983	molecular_function	protein dimerization activity
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006351	biological_process	DNA-templated transcription
A	0009274	cellular_component	peptidoglycan-based cell wall
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0046983	molecular_function	protein dimerization activity
b	0000428	cellular_component	DNA-directed RNA polymerase complex
b	0003677	molecular_function	DNA binding
b	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
b	0005737	cellular_component	cytoplasm
b	0005829	cellular_component	cytosol
b	0005886	cellular_component	plasma membrane
b	0006351	biological_process	DNA-templated transcription
b	0009274	cellular_component	peptidoglycan-based cell wall
b	0016779	molecular_function	nucleotidyltransferase activity
b	0034062	molecular_function	5'-3' RNA polymerase activity
b	0046983	molecular_function	protein dimerization activity
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006351	biological_process	DNA-templated transcription
B	0009274	cellular_component	peptidoglycan-based cell wall
B	0016779	molecular_function	nucleotidyltransferase activity
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0046983	molecular_function	protein dimerization activity
c	0000428	cellular_component	DNA-directed RNA polymerase complex
c	0003677	molecular_function	DNA binding
c	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
c	0005515	molecular_function	protein binding
c	0005829	cellular_component	cytosol
c	0005886	cellular_component	plasma membrane
c	0006351	biological_process	DNA-templated transcription
c	0009274	cellular_component	peptidoglycan-based cell wall
c	0016779	molecular_function	nucleotidyltransferase activity
c	0032549	molecular_function	ribonucleoside binding
c	0034062	molecular_function	5'-3' RNA polymerase activity
c	0046677	biological_process	response to antibiotic
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006351	biological_process	DNA-templated transcription
C	0009274	cellular_component	peptidoglycan-based cell wall
C	0016779	molecular_function	nucleotidyltransferase activity
C	0032549	molecular_function	ribonucleoside binding
C	0034062	molecular_function	5'-3' RNA polymerase activity
C	0046677	biological_process	response to antibiotic
d	0000287	molecular_function	magnesium ion binding
d	0000428	cellular_component	DNA-directed RNA polymerase complex
d	0003677	molecular_function	DNA binding
d	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
d	0005829	cellular_component	cytosol
d	0005886	cellular_component	plasma membrane
d	0006351	biological_process	DNA-templated transcription
d	0008270	molecular_function	zinc ion binding
d	0009274	cellular_component	peptidoglycan-based cell wall
d	0016779	molecular_function	nucleotidyltransferase activity
d	0034062	molecular_function	5'-3' RNA polymerase activity
d	0046872	molecular_function	metal ion binding
D	0000287	molecular_function	magnesium ion binding
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006351	biological_process	DNA-templated transcription
D	0008270	molecular_function	zinc ion binding
D	0009274	cellular_component	peptidoglycan-based cell wall
D	0016779	molecular_function	nucleotidyltransferase activity
D	0034062	molecular_function	5'-3' RNA polymerase activity
D	0046872	molecular_function	metal ion binding
e	0000428	cellular_component	DNA-directed RNA polymerase complex
e	0003677	molecular_function	DNA binding
e	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
e	0005829	cellular_component	cytosol
e	0006351	biological_process	DNA-templated transcription
e	0009274	cellular_component	peptidoglycan-based cell wall
e	0016779	molecular_function	nucleotidyltransferase activity
e	0034062	molecular_function	5'-3' RNA polymerase activity
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0005829	cellular_component	cytosol
E	0006351	biological_process	DNA-templated transcription
E	0009274	cellular_component	peptidoglycan-based cell wall
E	0016779	molecular_function	nucleotidyltransferase activity
E	0034062	molecular_function	5'-3' RNA polymerase activity
f	0001666	biological_process	response to hypoxia
f	0003677	molecular_function	DNA binding
f	0003700	molecular_function	DNA-binding transcription factor activity
f	0005515	molecular_function	protein binding
f	0005829	cellular_component	cytosol
f	0005886	cellular_component	plasma membrane
f	0006352	biological_process	DNA-templated transcription initiation
f	0006355	biological_process	regulation of DNA-templated transcription
f	0009408	biological_process	response to heat
f	0009410	biological_process	response to xenobiotic stimulus
f	0016987	molecular_function	sigma factor activity
f	0043175	molecular_function	RNA polymerase core enzyme binding
f	0045893	biological_process	positive regulation of DNA-templated transcription
f	2000142	biological_process	regulation of DNA-templated transcription initiation
F	0001666	biological_process	response to hypoxia
F	0003677	molecular_function	DNA binding
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0005515	molecular_function	protein binding
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006352	biological_process	DNA-templated transcription initiation
F	0006355	biological_process	regulation of DNA-templated transcription
F	0009408	biological_process	response to heat
F	0009410	biological_process	response to xenobiotic stimulus
F	0016987	molecular_function	sigma factor activity
F	0043175	molecular_function	RNA polymerase core enzyme binding
F	0045893	biological_process	positive regulation of DNA-templated transcription
F	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PROSITE/UniProt

site_id	PS00715
Number of Residues	14
Details	SIGMA70_1 Sigma-70 factors family signature 1. DLIQeGnLGLIrAM

Chain	Residue	Details
F	ASP114-MET127

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site_id	PS00716
Number of Residues	27
Details	SIGMA70_2 Sigma-70 factors family signature 2. TldQIGklfgLSrerVrQIErdvMskL

Chain	Residue	Details
F	THR283-LEU309

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site_id	PS00867
Number of Residues	8
Details	CPSASE_2 Carbamoyl-phosphate synthase subdomain signature 2. LLEANLRL

Chain	Residue	Details
F	LEU90-LEU97

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site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKLAGrHGNKGV

Chain	Residue	Details
C	GLY882-VAL894

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	38
Details	DNA_BIND: H-T-H motif => ECO:0000250

Chain	Residue	Details
F	LEU284-ARG303
D	CYS975
D	CYS978
d	CYS60
d	CYS62
d	CYS75
d	CYS78
d	ASP535
d	ASP537
d	ASP539
d	CYS891
f	LEU284-ARG303
d	CYS968
d	CYS975
d	CYS978
D	CYS75
D	CYS78
D	ASP535
D	ASP537
D	ASP539
D	CYS891
D	CYS968

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