7PQ0

Crystal structure of the Burkholderia Lethal Factor 1 (BLF1) C94S inactive mutant in complex with human eIF4A - Crystal form B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Burkholderia Lethal Factor 1 (BLF1)	polymer	211	23343.9	1	UniProt (Q63UP7) Pfam (PF17752) In PDB	Burkholderia pseudomallei (strain K96243)
2	B	Eukaryotic initiation factor 4A-I	polymer	394	45146.8	1	UniProt (P60842) Pfam (PF00270) Pfam (PF00271) In PDB	Homo sapiens (Human)	eIF-4A-I,eIF4A-I,ATP-dependent RNA helicase eIF4A-1

Sequence modifications

A: 1 - 211 (UniProt: Q63UP7)

PDB	External Database	Details
Ser 94	Cys 94	engineered mutation

B: 20 - 406 (UniProt: P60842)

PDB	External Database	Details
Met 13	-	initiating methionine
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	68490.7
All*	Total formula weight	68490.7

*Water molecules are not included.