7PQ0
Crystal structure of the Burkholderia Lethal Factor 1 (BLF1) C94S inactive mutant in complex with human eIF4A - Crystal form B
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0051097 | biological_process | negative regulation of helicase activity |
| B | 0000339 | molecular_function | RNA cap binding |
| B | 0002183 | biological_process | cytoplasmic translational initiation |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0003724 | molecular_function | RNA helicase activity |
| B | 0003725 | molecular_function | double-stranded RNA binding |
| B | 0003729 | molecular_function | mRNA binding |
| B | 0003743 | molecular_function | translation initiation factor activity |
| B | 0004386 | molecular_function | helicase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006412 | biological_process | translation |
| B | 0006413 | biological_process | translational initiation |
| B | 0008135 | molecular_function | translation factor activity, RNA binding |
| B | 0016020 | cellular_component | membrane |
| B | 0016281 | cellular_component | eukaryotic translation initiation factor 4F complex |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0016887 | molecular_function | ATP hydrolysis activity |
| B | 0045944 | biological_process | positive regulation of transcription by RNA polymerase II |
| B | 0048471 | cellular_component | perinuclear region of cytoplasm |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0097165 | cellular_component | nuclear stress granule |
Functional Information from PROSITE/UniProt
| site_id | PS00039 |
| Number of Residues | 9 |
| Details | DEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. VLDEADEmL |
| Chain | Residue | Details |
| B | VAL180-LEU188 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00541 |
| Chain | Residue | Details |
| B | ALA76 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| B | LYS118 | |
| B | LYS174 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphothreonine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| B | THR158 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P60843 |
| Chain | Residue | Details |
| B | LYS193 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P60843 |
| Chain | Residue | Details |
| B | LYS238 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| B | LYS146 | |
| B | LYS225 | |
| B | LYS309 | |
| B | LYS369 | |
| B | LYS381 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:28112733 |
| Chain | Residue | Details |
| B | LYS238 |
