7PHR

Structure of a fully assembled T-cell receptor engaging a tumor-associated peptide-MHC I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	T-cell receptor alpha chain	polymer	251	27979.3	1		Homo sapiens (Human)
2	B	T-cell receptor beta chain	polymer	290	32750.7	1		Homo sapiens (Human)
3	C	T-cell surface glycoprotein CD3 gamma chain	polymer	122	13713.7	1	UniProt (P09693) Pfam (PF16680) In PDB	Homo sapiens (Human)	T-cell receptor T3 gamma chain
4	D	T-cell surface glycoprotein CD3 delta chain, green fluorescent protein	polymer	356	39858.2	1	UniProt (P04234) UniProt (P42212) Pfam (PF16680) Pfam (PF01353) In PDB	Homo sapiens (Human)	T-cell receptor T3 delta chain
5	E, e	T-cell surface glycoprotein CD3 epsilon chain	polymer	136	15316.2	2	UniProt (P07766) Pfam (PF16681) In PDB	Homo sapiens (Human)	T-cell surface antigen T3/Leu-4 epsilon chain
6	H	HLA class I histocompatibility antigen, A alpha chain	polymer	304	35054.6	1	UniProt (P04439) Pfam (PF00129) Pfam (PF07654) In PDB	Homo sapiens (Human)	Human leukocyte antigen A,HLA-A
7	L	Beta-2-microglobulin	polymer	101	11936.4	1	UniProt (P61769) Pfam (PF07654) In PDB	Homo sapiens (Human)
8	P	Tumor-associated antigentic peptide gp100	polymer	9	974.1	1	UniProt (P40967) In PDB	Homo sapiens	ME20-M,ME20M,Melanocyte protein Pmel 17,Melanocytes lineage-specific antigen GP100,Melanoma-associated ME20 antigen,P1,P100,Premelanosome protein,Silver locus protein homolog,Melanocyte protein PMEL
9	Z, z	T-cell surface glycoprotein CD3 zeta chain	polymer	36	4138.0	2	UniProt (P20963) Pfam (PF11628) In PDB	Homo sapiens (Human)	T-cell receptor T3 zeta chain
10	A, B, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

D: 1 - 111 (UniProt: P04234)

PDB	External Database	Details
Asp 112	-	linker
Pro 113	-	linker
Pro 114	-	linker
Val 115	-	linker
Ala 116	-	linker
Thr 117	-	linker

D: 118 - 356 (UniProt: P42212)

PDB	External Database	Details
Val 119	-	insertion
Leu 182	Phe 64	engineered mutation
Leu 349	His 231	conflict

H: 1 - 280 (UniProt: P04439)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 62	Gln 86	variant
Lys 66	Asn 90	variant
His 70	Gln 94	variant
His 74	Asp 98	variant
Val 95	Ile 119	variant
Arg 97	Ile 121	variant
Trp 107	Gly 131	variant
His 114	Arg 138	variant
Tyr 116	Asp 140	variant
Lys 127	Asn 151	variant
Thr 142	Ile 166	variant
His 145	Arg 169	variant
Val 152	Glu 176	variant
Glu 161	Asp 185	variant
Ala 184	Pro 208	variant
Ala 193	Pro 217	variant
Val 194	Ile 218	variant
Ser 207	Gly 231	variant
Gln 253	Glu 277	variant
Pro 276	Leu 300	variant
Glu 281	-	expression tag
Asp 282	-	expression tag
Gln 283	-	expression tag
Val 284	-	expression tag
Asp 285	-	expression tag
Pro 286	-	expression tag
Arg 287	-	expression tag
Leu 288	-	expression tag
Ile 289	-	expression tag
Asp 290	-	expression tag
Gly 291	-	expression tag
Lys 292	-	expression tag

L: 0 - 99 (UniProt: P61769)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	Ala 20	conflict

P: 1 - 9 (UniProt: P40967)

PDB	External Database	Details
Val 9	Ala 288	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	201175.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	202502.8

*Water molecules are not included.