7PD2

Crystal structure of the substrate-free radical SAM tyrosine lyase ThiH (2-iminoacetate synthase) from Thermosinus carboxydivorans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Thiazole biosynthesis protein ThiH	polymer	377	43035.1	2	UniProt (A1HPQ5) Pfam (PF04055) Pfam (PF06968) In PDB	Thermosinus carboxydivorans Nor1	2-iminoacetate synthase ThiH
2	A, B	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	A, B	5'-DEOXYADENOSINE	non-polymer		251.2	2	Chemie (5AD)
4	A, B	METHIONINE	non-polymer		149.2	2	Chemie (MET)
5	A, B	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	A, B	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
7		water	water		18.0	247	Chemie (HOH)

Sequence modifications

A, B: 2 - 367 (UniProt: A1HPQ5)

PDB	External Database	Details
Met -9	-	initiating methionine
Trp -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
Pro -5	-	expression tag
Gln -4	-	expression tag
Phe -3	-	expression tag
Glu -2	-	expression tag
Lys -1	-	expression tag
Ala 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	86070.1
Non-Polymers*	Number of molecules	15
	Total formula weight	2356.8
All*	Total formula weight	88427.0

*Water molecules are not included.