7PCH

Human carboxyhemoglobin bound to Staphylococcus aureus hemophore IsdB - 1:2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	Hemoglobin subunit alpha	polymer	141	15150.4	2	UniProt (P69905) Pfam (PF00042) In PDB	Homo sapiens (Human)	Alpha-globin,Hemoglobin alpha chain
2	B, D	Hemoglobin subunit beta	polymer	146	15890.2	2	UniProt (P68871) Pfam (PF00042) In PDB	Homo sapiens (Human)	Beta-globin,Hemoglobin beta chain
3	E, F	Iron-regulated surface determinant protein B	polymer	372	43393.1	2	UniProt (Q8NX66) Pfam (PF05031) Pfam (PF20861) In PDB	Staphylococcus aureus subsp. aureus MW2	Fur-regulated protein B,Staphylococcal iron-regulated protein H,Staphylococcus aureus surface protein J
4	A, B, C, D	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
5		water	water		18.0	510	Chemie (HOH)

Sequence modifications

E, F: 125 - 485 (UniProt: Q8NX66)

PDB	External Database	Details
Met 124	-	initiating methionine
Ser 486	-	expression tag
Ala 487	-	expression tag
Trp 488	-	expression tag
Ser 489	-	expression tag
His 490	-	expression tag
Pro 491	-	expression tag
Gln 492	-	expression tag
Phe 493	-	expression tag
Glu 494	-	expression tag
Lys 495	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	148867.4
Non-Polymers*	Number of molecules	4
	Total formula weight	2465.9
All*	Total formula weight	151333.3

*Water molecules are not included.