7OVG
The C146A variant of an amidase from Pyrococcus horikoshii with bound acetamide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | CN hydrolase domain-containing protein | polymer | 263 | 29950.5 | 2 | UniProt (O58376) Pfam (PF00795) In PDB | Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) | |
| 2 | A, B | ACETAMIDE | non-polymer | 59.1 | 2 | Chemie (ACM) | |||
| 3 | A, B | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 4 | water | water | 18.0 | 207 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 262 (UniProt: O58376)
| PDB | External Database | Details |
|---|---|---|
| His 0 | - | expression tag |
| Ala 146 | Cys 146 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 59901.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 189.0 | |
| All* | Total formula weight | 60090.0 |
