7OTC
Cryo-EM structure of an Escherichia coli 70S ribosome in complex with elongation factor G and the antibiotic Argyrin B
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (a) | 16S ribosomal RNA | polymer | 1533 | 497061.7 | 1 | GenBank (1758835854) | Escherichia coli BL21(DE3) | |
| 2 | B (b) | 30S ribosomal protein S2 | polymer | 226 | 25200.0 | 1 | UniProt (A0A140NFK2) | Escherichia coli BL21(DE3) | |
| 3 | C (c) | 30S ribosomal protein S3 | polymer | 206 | 23078.8 | 1 | UniProt (A0A140N4K1) | Escherichia coli BL21(DE3) | |
| 4 | D (d) | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (A0A140N548) | Escherichia coli BL21(DE3) | |
| 5 | E (e) | 30S ribosomal protein S5 | polymer | 157 | 16532.1 | 1 | UniProt (A0A140N6Z9) | Escherichia coli BL21(DE3) | |
| 6 | F (f) | 30S ribosomal protein S6 | polymer | 106 | 12326.3 | 1 | UniProt (A0A140NGG7) | Escherichia coli BL21(DE3) | |
| 7 | G (g) | 30S ribosomal protein S7 | polymer | 156 | 17619.4 | 1 | UniProt (A0A140N6W8) | Escherichia coli BL21(DE3) | |
| 8 | H (h) | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (A0A140N537) | Escherichia coli BL21(DE3) | |
| 9 | I (i) | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (A0A140N2Z9) | Escherichia coli BL21(DE3) | |
| 10 | J (j) | 30S ribosomal protein S10 | polymer | 99 | 11325.1 | 1 | UniProt (A0A140N6Y5) | Escherichia coli BL21(DE3) | |
| 11 | K (k) | 30S ribosomal protein S11 | polymer | 117 | 12487.2 | 1 | UniProt (A0A140N7L9) | Escherichia coli BL21(DE3) | |
| 12 | L (l) | 30S ribosomal protein S12 | polymer | 123 | 13683.1 | 1 | UniProt (A0A140N4H3) | Escherichia coli BL21(DE3) | |
| 13 | M (m) | 30S ribosomal protein S13 | polymer | 115 | 12738.9 | 1 | UniProt (A0A140N5B4) | Escherichia coli BL21(DE3) | |
| 14 | N (n) | 30S ribosomal protein S14 | polymer | 101 | 11606.6 | 1 | UniProt (A0A140N7K8) | Escherichia coli BL21(DE3) | |
| 15 | O (o) | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (A0A140N811) | Escherichia coli BL21(DE3) | |
| 16 | P (p) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (A0A140N7D8) | Escherichia coli BL21(DE3) | |
| 17 | Q (q) | 30S ribosomal protein S17 | polymer | 80 | 9263.9 | 1 | UniProt (A0A140N6Z6) | Escherichia coli BL21(DE3) | |
| 18 | R (r) | 30S ribosomal protein S18 | polymer | 67 | 7893.1 | 1 | UniProt (A0A140NGH1) | Escherichia coli BL21(DE3) | |
| 19 | S (s) | 30S ribosomal protein S19 | polymer | 92 | 10455.4 | 1 | UniProt (A0A140N528) | Escherichia coli BL21(DE3) | |
| 20 | T (t) | 30S ribosomal protein S20 | polymer | 86 | 9577.3 | 1 | UniProt (A0A140NFU3) | Escherichia coli BL21(DE3) | |
| 21 | U (u) | 30S ribosomal protein S21 | polymer | 70 | 8392.8 | 1 | UniProt (A0A140N8B4) | Escherichia coli BL21(DE3) | |
| 22 | V (A) | 23S ribosomal RNA | polymer | 2903 | 941505.4 | 1 | Escherichia coli BL21(DE3) | ||
| 23 | W (B) | 5S ribosomal RNA | polymer | 120 | 38790.1 | 1 | GenBank (1845283434) | Escherichia coli BL21(DE3) | |
| 24 | X (C) | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (A0A140N7J1) | Escherichia coli BL21(DE3) | |
| 25 | Y (D) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (A0A140N3G7) | Escherichia coli BL21(DE3) | |
| 26 | Z (E) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (A0A140N5K8) | Escherichia coli BL21(DE3) | |
| 27 | AA (F) | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (A0A140N5A3) | Escherichia coli BL21(DE3) | |
| 28 | BA (G) | 50S ribosomal protein L6 | polymer | 176 | 18801.6 | 1 | UniProt (A0A140N2T1) | Escherichia coli BL21(DE3) | |
| 29 | CA (J) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (A0A140N598) | Escherichia coli BL21(DE3) | |
| 30 | DA (K) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (A0A140N3H4) | Escherichia coli BL21(DE3) | |
| 31 | EA (L) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (A0A140N711) | Escherichia coli BL21(DE3) | |
| 32 | FA (M) | 50S ribosomal protein L16 | polymer | 136 | 15327.3 | 1 | UniProt (A0A140N6Z2) | Escherichia coli BL21(DE3) | |
| 33 | GA (N) | 50S ribosomal protein L17 | polymer | 120 | 13721.9 | 1 | UniProt (A0A140N4M0) | Escherichia coli BL21(DE3) | |
| 34 | HA (O) | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (A0A140N4L0) | Escherichia coli BL21(DE3) | |
| 35 | IA (P) | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (A0A140N6T7) | Escherichia coli BL21(DE3) | |
| 36 | JA (Q) | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (A0A140NBS1) | Escherichia coli BL21(DE3) | |
| 37 | KA (R) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (A0A140N5D7) | Escherichia coli BL21(DE3) | |
| 38 | LA (S) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (A0A140N2S3) | Escherichia coli BL21(DE3) | |
| 39 | MA (T) | 50S ribosomal protein L23 | polymer | 93 | 10546.5 | 1 | UniProt (C6EGE8) | Escherichia coli BL21(DE3) | |
| 40 | NA (U) | 50S ribosomal protein L24 | polymer | 102 | 11078.9 | 1 | UniProt (A0A140N5L7) | Escherichia coli BL21(DE3) | |
| 41 | OA (V) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (A0A140N846) | Escherichia coli BL21(DE3) | |
| 42 | PA (W) | 50S ribosomal protein L27 | polymer | 84 | 9015.3 | 1 | UniProt (A0A140N340) | Escherichia coli BL21(DE3) | |
| 43 | QA (X) | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (A0A140N3L9) | Escherichia coli BL21(DE3) | |
| 44 | RA (Y) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (A0A140N7F5) | Escherichia coli BL21(DE3) | |
| 45 | SA (Z) | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (A0A140N7G4) | Escherichia coli BL21(DE3) | |
| 46 | TA (5) | 50S ribosomal protein L31 | polymer | 67 | 7613.8 | 1 | UniProt (A0A140SS71) | Escherichia coli BL21(DE3) | |
| 47 | UA (0) | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (A0A140NAZ2) | Escherichia coli BL21(DE3) | |
| 48 | VA (1) | 50S ribosomal protein L33 | polymer | 50 | 5814.8 | 1 | UniProt (A0A140N5Z7) | Escherichia coli BL21(DE3) | |
| 49 | WA (2) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (A0A140NHV0) | Escherichia coli BL21(DE3) | |
| 50 | XA (3) | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (A0A140N9E3) | Escherichia coli BL21(DE3) | |
| 51 | YA (4) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (A0A140N5M6) | Escherichia coli BL21(DE3) | |
| 52 | ZA (w) | Elongation factor G | polymer | 704 | 77571.0 | 1 | UniProt (A0A140N7C7) | Escherichia coli BL21(DE3) | EF-G |
| 53 | AB, AC, AD, AE, AF... (a, A, B, n, C...) | MAGNESIUM ION | non-polymer | 24.3 | 292 | Chemie (MG) PubChem (888) | |||
| 54 | KD (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 55 | LD, MD, ND, OD, PD... (A) | 1,4-DIAMINOBUTANE | non-polymer | 88.2 | 8 | Chemie (PUT) PubChem (1045) | |||
| 56 | SD, TD (A) | SPERMIDINE | non-polymer | 145.2 | 2 | Chemie (SPD) PubChem (1102) | |||
| 57 | PM, RM (5, 4) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 58 | TM (w) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 59 | UM (w) | Argyrin B | non-polymer | 838.9 | 1 | Chemie (1I7) |
Sequence modifications
e: 10 - 166 (UniProt: A0A140N6Z9)
g: 2 - 156 (UniProt: A0A140N6W8)
n: 2 - 101 (UniProt: A0A140N7K8)
L: 1 - 144 (UniProt: A0A140N711)
w: 2 - 704 (UniProt: A0A140N7C7)
| PDB | External Database | Details |
|---|---|---|
| Glu 151 | Ala 151 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Leu 157 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| PDB | External Database | Details |
|---|---|---|
| Ile 77 | Val 77 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| Thr 237 | Tyr 237 | variant |
| Asp 258 | Asn 258 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 52 |
| Total formula weight | 2178645.4 | |
| Non-Polymers* | Number of molecules | 307 |
| Total formula weight | 10012.9 | |
| All* | Total formula weight | 2188658.3 |
