7OTC
Cryo-EM structure of an Escherichia coli 70S ribosome in complex with elongation factor G and the antibiotic Argyrin B
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (a) | 16S ribosomal RNA | polymer | 1533 | 497061.7 | 1 | GenBank (1758835854) | Escherichia coli BL21(DE3) | |
| 2 | B (b) | 30S ribosomal protein S2 | polymer | 226 | 25200.0 | 1 | UniProt (A0A140NFK2) | Escherichia coli BL21(DE3) | |
| 3 | C (c) | 30S ribosomal protein S3 | polymer | 206 | 23078.8 | 1 | UniProt (A0A140N4K1) | Escherichia coli BL21(DE3) | |
| 4 | D (d) | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (A0A140N548) | Escherichia coli BL21(DE3) | |
| 5 | E (e) | 30S ribosomal protein S5 | polymer | 157 | 16532.1 | 1 | UniProt (A0A140N6Z9) | Escherichia coli BL21(DE3) | |
| 6 | F (f) | 30S ribosomal protein S6 | polymer | 106 | 12326.3 | 1 | UniProt (A0A140NGG7) | Escherichia coli BL21(DE3) | |
| 7 | G (g) | 30S ribosomal protein S7 | polymer | 156 | 17619.4 | 1 | UniProt (A0A140N6W8) | Escherichia coli BL21(DE3) | |
| 8 | H (h) | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (A0A140N537) | Escherichia coli BL21(DE3) | |
| 9 | I (i) | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (A0A140N2Z9) | Escherichia coli BL21(DE3) | |
| 10 | J (j) | 30S ribosomal protein S10 | polymer | 99 | 11325.1 | 1 | UniProt (A0A140N6Y5) | Escherichia coli BL21(DE3) | |
| 11 | K (k) | 30S ribosomal protein S11 | polymer | 117 | 12487.2 | 1 | UniProt (A0A140N7L9) | Escherichia coli BL21(DE3) | |
| 12 | L (l) | 30S ribosomal protein S12 | polymer | 123 | 13683.1 | 1 | UniProt (A0A140N4H3) | Escherichia coli BL21(DE3) | |
| 13 | M (m) | 30S ribosomal protein S13 | polymer | 115 | 12738.9 | 1 | UniProt (A0A140N5B4) | Escherichia coli BL21(DE3) | |
| 14 | N (n) | 30S ribosomal protein S14 | polymer | 101 | 11606.6 | 1 | UniProt (A0A140N7K8) | Escherichia coli BL21(DE3) | |
| 15 | O (o) | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (A0A140N811) | Escherichia coli BL21(DE3) | |
| 16 | P (p) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (A0A140N7D8) | Escherichia coli BL21(DE3) | |
| 17 | Q (q) | 30S ribosomal protein S17 | polymer | 80 | 9263.9 | 1 | UniProt (A0A140N6Z6) | Escherichia coli BL21(DE3) | |
| 18 | R (r) | 30S ribosomal protein S18 | polymer | 67 | 7893.1 | 1 | UniProt (A0A140NGH1) | Escherichia coli BL21(DE3) | |
| 19 | S (s) | 30S ribosomal protein S19 | polymer | 92 | 10455.4 | 1 | UniProt (A0A140N528) | Escherichia coli BL21(DE3) | |
| 20 | T (t) | 30S ribosomal protein S20 | polymer | 86 | 9577.3 | 1 | UniProt (A0A140NFU3) | Escherichia coli BL21(DE3) | |
| 21 | U (u) | 30S ribosomal protein S21 | polymer | 70 | 8392.8 | 1 | UniProt (A0A140N8B4) | Escherichia coli BL21(DE3) | |
| 22 | V (A) | 23S ribosomal RNA | polymer | 2903 | 941505.4 | 1 | Escherichia coli BL21(DE3) | ||
| 23 | W (B) | 5S ribosomal RNA | polymer | 120 | 38790.1 | 1 | GenBank (1845283434) | Escherichia coli BL21(DE3) | |
| 24 | X (C) | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (A0A140N7J1) | Escherichia coli BL21(DE3) | |
| 25 | Y (D) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (A0A140N3G7) | Escherichia coli BL21(DE3) | |
| 26 | Z (E) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (A0A140N5K8) | Escherichia coli BL21(DE3) | |
| 27 | AA (F) | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (A0A140N5A3) | Escherichia coli BL21(DE3) | |
| 28 | BA (G) | 50S ribosomal protein L6 | polymer | 176 | 18801.6 | 1 | UniProt (A0A140N2T1) | Escherichia coli BL21(DE3) | |
| 29 | CA (J) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (A0A140N598) | Escherichia coli BL21(DE3) | |
| 30 | DA (K) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (A0A140N3H4) | Escherichia coli BL21(DE3) | |
| 31 | EA (L) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (A0A140N711) | Escherichia coli BL21(DE3) | |
| 32 | FA (M) | 50S ribosomal protein L16 | polymer | 136 | 15327.3 | 1 | UniProt (A0A140N6Z2) | Escherichia coli BL21(DE3) | |
| 33 | GA (N) | 50S ribosomal protein L17 | polymer | 120 | 13721.9 | 1 | UniProt (A0A140N4M0) | Escherichia coli BL21(DE3) | |
| 34 | HA (O) | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (A0A140N4L0) | Escherichia coli BL21(DE3) | |
| 35 | IA (P) | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (A0A140N6T7) | Escherichia coli BL21(DE3) | |
| 36 | JA (Q) | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (A0A140NBS1) | Escherichia coli BL21(DE3) | |
| 37 | KA (R) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (A0A140N5D7) | Escherichia coli BL21(DE3) | |
| 38 | LA (S) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (A0A140N2S3) | Escherichia coli BL21(DE3) | |
| 39 | MA (T) | 50S ribosomal protein L23 | polymer | 93 | 10546.5 | 1 | UniProt (C6EGE8) | Escherichia coli BL21(DE3) | |
| 40 | NA (U) | 50S ribosomal protein L24 | polymer | 102 | 11078.9 | 1 | UniProt (A0A140N5L7) | Escherichia coli BL21(DE3) | |
| 41 | OA (V) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (A0A140N846) | Escherichia coli BL21(DE3) | |
| 42 | PA (W) | 50S ribosomal protein L27 | polymer | 84 | 9015.3 | 1 | UniProt (A0A140N340) | Escherichia coli BL21(DE3) | |
| 43 | QA (X) | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (A0A140N3L9) | Escherichia coli BL21(DE3) | |
| 44 | RA (Y) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (A0A140N7F5) | Escherichia coli BL21(DE3) | |
| 45 | SA (Z) | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (A0A140N7G4) | Escherichia coli BL21(DE3) | |
| 46 | TA (5) | 50S ribosomal protein L31 | polymer | 67 | 7613.8 | 1 | UniProt (A0A140SS71) | Escherichia coli BL21(DE3) | |
| 47 | UA (0) | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (A0A140NAZ2) | Escherichia coli BL21(DE3) | |
| 48 | VA (1) | 50S ribosomal protein L33 | polymer | 50 | 5814.8 | 1 | UniProt (A0A140N5Z7) | Escherichia coli BL21(DE3) | |
| 49 | WA (2) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (A0A140NHV0) | Escherichia coli BL21(DE3) | |
| 50 | XA (3) | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (A0A140N9E3) | Escherichia coli BL21(DE3) | |
| 51 | YA (4) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (A0A140N5M6) | Escherichia coli BL21(DE3) | |
| 52 | ZA (w) | Elongation factor G | polymer | 704 | 77571.0 | 1 | UniProt (A0A140N7C7) | Escherichia coli BL21(DE3) | EF-G |
| 53 | AB, AC, AD, AE, AF... (a, A, B, n, C...) | MAGNESIUM ION | non-polymer | 24.3 | 292 | Chemie (MG) | |||
| 54 | KD (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 55 | LD, MD, ND, OD, PD... (A) | 1,4-DIAMINOBUTANE | non-polymer | 88.2 | 8 | Chemie (PUT) | |||
| 56 | SD, TD (A) | SPERMIDINE | non-polymer | 145.2 | 2 | Chemie (SPD) | |||
| 57 | PM, RM (5, 4) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 58 | TM (w) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 59 | UM (w) | Argyrin B | non-polymer | 838.9 | 1 | Chemie (1I7) |
Sequence modifications
e: 10 - 166 (UniProt: A0A140N6Z9)
g: 2 - 156 (UniProt: A0A140N6W8)
n: 2 - 101 (UniProt: A0A140N7K8)
L: 1 - 144 (UniProt: A0A140N711)
w: 2 - 704 (UniProt: A0A140N7C7)
| PDB | External Database | Details |
|---|---|---|
| Glu 151 | Ala 151 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Leu 157 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| PDB | External Database | Details |
|---|---|---|
| Ile 77 | Val 77 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| Thr 237 | Tyr 237 | variant |
| Asp 258 | Asn 258 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 52 |
| Total formula weight | 2178645.4 | |
| Non-Polymers* | Number of molecules | 307 |
| Total formula weight | 10012.9 | |
| All* | Total formula weight | 2188658.3 |
