7OQY
Cryo-EM structure of the cellular negative regulator TFS4 bound to the archaeal RNA polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit A' | polymer | 880 | 99929.1 | 1 | UniProt (P11512) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOA' (RPO1N) |
| 2 | B (B) | DNA-directed RNA polymerase subunit B | polymer | 1126 | 126647.1 | 1 | UniProt (P11513) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOB (RPO2) |
| 3 | C (C) | DNA-directed RNA polymerase subunit A'' | polymer | 393 | 44507.4 | 1 | UniProt (P11514) Pfam (PF04998) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOA'' (RPO1C) |
| 4 | D (D) | DNA-directed RNA polymerase subunit D | polymer | 264 | 29858.8 | 1 | UniProt (P39471) Pfam (PF01193) Pfam (PF01000) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOD (RPO3) |
| 5 | E (E) | DNA-directed RNA polymerase subunit E | polymer | 183 | 20496.8 | 1 | UniProt (P39466) Pfam (PF03876) Pfam (PF00575) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOE (RPO7) |
| 6 | F (F) | DNA-directed RNA polymerase, subunit F | polymer | 114 | 13071.0 | 1 | UniProt (Q4JB12) Pfam (PF03874) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOF (RPO4) |
| 7 | G (G) | DNA-directed RNA polymerase, subunit G | polymer | 130 | 15292.8 | 1 | UniProt (Q4JAY4) Pfam (PF16992) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOG (RPO8) |
| 8 | H (H) | DNA-directed RNA polymerase subunit H | polymer | 84 | 9477.1 | 1 | UniProt (P11521) Pfam (PF01191) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOH (RPO5) |
| 9 | I (K) | DNA-directed RNA polymerase subunit K | polymer | 89 | 10252.0 | 1 | UniProt (P39463) Pfam (PF01192) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOK (RPO6) |
| 10 | J (L) | DNA-directed RNA polymerase subunit L | polymer | 90 | 10061.8 | 1 | UniProt (P46217) Pfam (PF13656) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOL (RPO11) |
| 11 | K (N) | DNA-directed RNA polymerase subunit N | polymer | 66 | 7673.1 | 1 | UniProt (P39472) Pfam (PF01194) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPON (RPO10) |
| 12 | L (P) | DNA-directed RNA polymerase subunit P | polymer | 48 | 5662.9 | 1 | UniProt (Q4JAE8) Pfam (PF03604) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPOP (RPO12) |
| 13 | M (Q) | Conserved protein | polymer | 105 | 12331.7 | 1 | UniProt (Q4JAJ6) Pfam (PF12136) | Sulfolobus acidocaldarius DSM 639 | DNA-dependent RNA polymerase subunit RPO13 |
| 14 | N (Y) | DNA-directed RNA polymerase, subunit M (RpoM-2) | polymer | 92 | 10422.4 | 1 | UniProt (Q97X43) Pfam (PF02150) | Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) | DNA-directed RNA polymerase subunit RpoM-2 (TFS4) |
| 15 | O (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 16 | P, Q, R, S, U... (A, B, N, P, Y) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 17 | T (D) | FE3-S4 CLUSTER | non-polymer | 295.8 | 1 | Chemie (F3S) |
Sequence modifications
B: 1 - 1126 (UniProt: P11513)
G: 1 - 124 (UniProt: Q4JAY4)
| PDB | External Database | Details |
|---|---|---|
| Ile 772 | Val 772 | variant |
| PDB | External Database | Details |
|---|---|---|
| His 125 | - | expression tag |
| His 126 | - | expression tag |
| His 127 | - | expression tag |
| His 128 | - | expression tag |
| His 129 | - | expression tag |
| His 130 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 415683.8 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 843.4 | |
| All* | Total formula weight | 416527.1 |
