7ONI

Structure of Neddylated CUL5 C-terminal region-RBX2-ARIH2*

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C	Cullin-5	polymer	780	91085.3	1	UniProt (Q93034) Pfam (PF00888) Pfam (PF10557) In PDB	Homo sapiens (Human)	CUL-5,Vasopressin-activated calcium-mobilizing receptor 1,VACM-1
2	H	E3 ubiquitin-protein ligase ARIH2	polymer	495	57879.8	1	UniProt (O95376) Pfam (PF00097) Pfam (PF01485) Pfam (PF22191) Pfam (PF19422) In PDB	Homo sapiens (Human)	ARI-2,Protein ariadne-2 homolog,RING-type E3 ubiquitin transferase ARIH2,Triad1 protein
3	R	RING-box protein 2	polymer	111	12425.1	1	UniProt (Q9UBF6) Pfam (PF12678) In PDB	Homo sapiens (Human)	Rbx2,CKII beta-binding protein 1,CKBBP1,RING finger protein 7,Regulator of cullins 2,Sensitive to apoptosis gene protein
4	N	NEDD8	polymer	83	9230.7	1	UniProt (Q15843) Pfam (PF00240) In PDB	Homo sapiens (Human)	Neddylin,Neural precursor cell expressed developmentally down-regulated protein 8,NEDD-8,Ubiquitin-like protein Nedd8
5	H, R	ZINC ION	non-polymer		65.4	7	Chemie (ZN)

Sequence modifications

H: 1 - 493 (UniProt: O95376)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 381	Leu 381	engineered mutation
Ala 382	Glu 382	engineered mutation
Ala 455	Glu 455	engineered mutation

R: 5 - 113 (UniProt: Q9UBF6)

PDB	External Database	Details
Gly 3	-	expression tag
Ser 4	-	expression tag

N: 1 - 81 (UniProt: Q15843)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	170620.9
Non-Polymers*	Number of molecules	7
	Total formula weight	457.9
All*	Total formula weight	171078.7

*Water molecules are not included.