7OKQ

Cryo-EM Structure of the DDB1-DCAF1-CUL4A-RBX1 Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, E, I, M)	DNA damage-binding protein 1	polymer	1159	129394.9	4	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178)	Homo sapiens (Human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
2	B, F, J, N (B, F, J, N)	DDB1- and CUL4-associated factor 1	polymer	1525	171279.1	4	UniProt (Q9Y4B6)	Homo sapiens (Human)	HIV-1 Vpr-binding protein,VprBP,Serine/threonine-protein kinase VPRBP,Vpr-interacting protein
3	C, G, K, O (C, G, K, O)	Cullin-4A	polymer	742	86702.8	4	UniProt (Q13619) Pfam (PF00888) Pfam (PF10557)	Homo sapiens (Human)	CUL-4A
4	D, H, L, P (D, H, L, P)	E3 ubiquitin-protein ligase RBX1	polymer	116	13497.2	4	UniProt (P62877) Pfam (PF12678)	Homo sapiens (Human)	E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1

Sequence modifications

A, E, I, M: 1 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Met -18	-	initiating methionine
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

B, F, J, N: 2 - 1507 (UniProt: Q9Y4B6)

PDB	External Database	Details
Met -17	-	initiating methionine
Ala -16	-	expression tag
Ser -15	-	expression tag
Trp -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
Pro -11	-	expression tag
Gln -10	-	expression tag
Phe -9	-	expression tag
Glu -8	-	expression tag
Lys -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag

C, G, K, O: 35 - 759 (UniProt: Q13619)

PDB	External Database	Details
Met 18	-	initiating methionine
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
Val 25	-	expression tag
Asp 26	-	expression tag
Glu 27	-	expression tag
Glu 28	-	expression tag
Asn 29	-	expression tag
Leu 30	-	expression tag
Tyr 31	-	expression tag
Phe 32	-	expression tag
Gln 33	-	expression tag
Gly 34	-	expression tag
Arg 37	Ala 37	conflict

D, H, L, P: -7 - 108 (UniProt: P62877)

PDB	External Database	Details
His -6	-	insertion
His -5	Ala 2	conflict
His -4	Ala 3	conflict
His -3	Ala 4	conflict
His -2	Met 5	conflict
His -1	Asp 6	conflict
Glu 2	-	insertion
Asn 3	-	insertion
Leu 4	-	insertion
Tyr 5	-	insertion
Phe 6	-	insertion
Gln 7	-	insertion
Gly 8	-	insertion
Gly 9	Thr 9	conflict
Gly 10	Pro 10	conflict
Arg 11	Ser 11	conflict

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	1603496.3
All*	Total formula weight	1603496.3

*Water molecules are not included.