7OB9
Cryo-EM structure of human RNA Polymerase I in elongation state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase I subunit RPA1 | polymer | 1720 | 195069.0 | 1 | UniProt (O95602) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Homo sapiens (Human) | RNA polymerase I subunit A1,A190,DNA-directed RNA polymerase I largest subunit,DNA-directed RNA polymerase I subunit A,RNA polymerase I 194 kDa subunit,RPA194 |
| 2 | B (B) | DNA-directed RNA polymerase I subunit RPA2 | polymer | 1135 | 128379.2 | 1 | UniProt (Q9H9Y6) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF06883) | Homo sapiens (Human) | RNA polymerase I subunit 2,DNA-directed RNA polymerase I 135 kDa polypeptide,RPA135 |
| 3 | C (C) | DNA-directed RNA polymerases I and III subunit RPAC1 | polymer | 346 | 39301.7 | 1 | UniProt (O15160) Pfam (PF01193) Pfam (PF01000) | Homo sapiens (Human) | RNA polymerases I and III subunit AC1,AC40,DNA-directed RNA polymerases I and III 40 kDa polypeptide,RPA40,RPA39,RPC40 |
| 4 | D (E) | DNA-directed RNA polymerases I, II, and III subunit RPABC1 | polymer | 210 | 24644.3 | 1 | UniProt (P19388) Pfam (PF03871) Pfam (PF01191) | Homo sapiens (Human) | RNA polymerases I,II,and III subunit ABC1,DNA-directed RNA polymerase II 23 kDa polypeptide,DNA-directed RNA polymerase II subunit E,RPB5 homolog,XAP4 |
| 5 | E (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14491.0 | 1 | UniProt (P61218) Pfam (PF01192) | Homo sapiens (Human) | RNA polymerases I,II,and III subunit ABC2,DNA-directed RNA polymerase II subunit F,DNA-directed RNA polymerases I,and III 14.4 kDa polypeptide,RPABC14.4,RPB14.4,RPB6 homolog,RPC15 |
| 6 | F (G) | DNA-directed RNA polymerase I subunit RPA43 | polymer | 338 | 37490.4 | 1 | UniProt (Q3B726) Pfam (PF03876) Pfam (PF17875) | Homo sapiens (Human) | DNA-directed RNA polymerase I subunit F,Twist neighbor protein |
| 7 | G (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (P52434) Pfam (PF03870) | Homo sapiens (Human) | RNA polymerases I,II,and III subunit ABC3,DNA-directed RNA polymerase II subunit H,DNA-directed RNA polymerases I,and III 17.1 kDa polypeptide,RPB17,RPB8 homolog,hRPB8 |
| 8 | H (I) | DNA-directed RNA polymerase I subunit RPA12 | polymer | 126 | 13917.7 | 1 | UniProt (Q9P1U0) Pfam (PF01096) | Homo sapiens (Human) | DNA-directed RNA polymerase I subunit H,Zinc ribbon domain-containing protein 1 |
| 9 | I (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (P62875) Pfam (PF01194) | Homo sapiens (Human) | RNA polymerases I,II,and III subunit ABC5,DNA-directed RNA polymerase III subunit L,RNA polymerase II 7.6 kDa subunit,RPB7.6,RPB10 homolog |
| 10 | J (K) | DNA-directed RNA polymerases I and III subunit RPAC2 | polymer | 133 | 15259.2 | 1 | UniProt (P0DPB6) Pfam (PF13656) | Homo sapiens (Human) | RNA polymerases I and III subunit AC2,AC19,DNA-directed RNA polymerase I subunit D,RNA polymerase I 16 kDa subunit,RPA16,RPC16,hRPA19 |
| 11 | K (L) | DNA-directed RNA polymerases I, II, and III subunit RPABC4 | polymer | 58 | 7018.2 | 1 | UniProt (P53803) Pfam (PF03604) | Homo sapiens (Human) | RNA polymerases I,II,and III subunit ABC4,ABC10-alpha,DNA-directed RNA polymerase II subunit K,RNA polymerase II 7.0 kDa subunit,RPB7.0,RPB10alpha |
| 12 | L (N) | DNA-directed RNA polymerase I subunit RPA34 | polymer | 510 | 55065.5 | 1 | UniProt (O15446) Pfam (PF08208) | Homo sapiens (Human) | A34.5,Antisense to ERCC-1 protein,ASE-1,CD3-epsilon-associated protein,CD3E-associated protein,DNA-directed RNA polymerase I subunit G,RNA polymerase I-associated factor PAF49 |
| 13 | M (M) | DNA-directed RNA polymerase I subunit RPA49 | polymer | 419 | 47330.2 | 1 | UniProt (Q9GZS1) Pfam (PF06870) | Homo sapiens (Human) | RNA polymerase I subunit A49,DNA-directed RNA polymerase I subunit E,RNA polymerase I-associated factor 1,RNA polymerase I-associated factor 53 |
| 14 | N (R) | RNA | polymer | 29 | 9275.6 | 1 | Homo sapiens (Human) | ||
| 15 | O (S) | DNA non-template strand | polymer | 43 | 13265.5 | 1 | Homo sapiens (Human) | ||
| 16 | P (T) | DNA template strand | polymer | 43 | 13274.5 | 1 | Homo sapiens (Human) | ||
| 17 | Q, R, T, U, V... (A, B, I, J, L) | ZINC ION | non-polymer | 65.4 | 6 | Chemie (ZN) | |||
| 18 | S (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
E: 1 - 210 (UniProt: P19388)
| PDB | External Database | Details |
|---|---|---|
| Phe 44 | Ser 44 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 638599.6 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 416.8 | |
| All* | Total formula weight | 639016.4 |
