7OB9

Cryo-EM structure of human RNA Polymerase I in elongation state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0001054	molecular_function	RNA polymerase I activity
A	0001055	molecular_function	RNA polymerase II activity
A	0001056	molecular_function	RNA polymerase III activity
A	0001057	molecular_function	RNA polymerase IV activity
A	0001058	molecular_function	RNA polymerase V activity
A	0001059	biological_process	transcription by RNA polymerase IV
A	0001060	biological_process	transcription by RNA polymerase V
A	0001065	molecular_function	mitochondrial single subunit type RNA polymerase activity
A	0001066	molecular_function	plastid single subunit type RNA polymerase activity
A	0003677	molecular_function	DNA binding
A	0003682	molecular_function	chromatin binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005694	cellular_component	chromosome
A	0005730	cellular_component	nucleolus
A	0005736	cellular_component	RNA polymerase I complex
A	0006351	biological_process	DNA-templated transcription
A	0006360	biological_process	transcription by RNA polymerase I
A	0006366	biological_process	transcription by RNA polymerase II
A	0006383	biological_process	transcription by RNA polymerase III
A	0006390	biological_process	mitochondrial transcription
A	0008270	molecular_function	zinc ion binding
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0042793	biological_process	plastid transcription
A	0046872	molecular_function	metal ion binding
A	0071667	molecular_function	DNA/RNA hybrid binding
A	1904750	biological_process	negative regulation of protein localization to nucleolus
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0001054	molecular_function	RNA polymerase I activity
B	0001055	molecular_function	RNA polymerase II activity
B	0001056	molecular_function	RNA polymerase III activity
B	0001057	molecular_function	RNA polymerase IV activity
B	0001058	molecular_function	RNA polymerase V activity
B	0001059	biological_process	transcription by RNA polymerase IV
B	0001060	biological_process	transcription by RNA polymerase V
B	0001065	molecular_function	mitochondrial single subunit type RNA polymerase activity
B	0001066	molecular_function	plastid single subunit type RNA polymerase activity
B	0001650	cellular_component	fibrillar center
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005694	cellular_component	chromosome
B	0005730	cellular_component	nucleolus
B	0005736	cellular_component	RNA polymerase I complex
B	0005829	cellular_component	cytosol
B	0006351	biological_process	DNA-templated transcription
B	0006360	biological_process	transcription by RNA polymerase I
B	0006366	biological_process	transcription by RNA polymerase II
B	0006383	biological_process	transcription by RNA polymerase III
B	0006390	biological_process	mitochondrial transcription
B	0007566	biological_process	embryo implantation
B	0008270	molecular_function	zinc ion binding
B	0009303	biological_process	rRNA transcription
B	0014029	biological_process	neural crest formation
B	0016779	molecular_function	nucleotidyltransferase activity
B	0017126	biological_process	nucleologenesis
B	0032549	molecular_function	ribonucleoside binding
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0042793	biological_process	plastid transcription
B	0046872	molecular_function	metal ion binding
B	0071667	molecular_function	DNA/RNA hybrid binding
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0001054	molecular_function	RNA polymerase I activity
C	0001056	molecular_function	RNA polymerase III activity
C	0001650	cellular_component	fibrillar center
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005666	cellular_component	RNA polymerase III complex
C	0005730	cellular_component	nucleolus
C	0005736	cellular_component	RNA polymerase I complex
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006351	biological_process	DNA-templated transcription
C	0006360	biological_process	transcription by RNA polymerase I
C	0006383	biological_process	transcription by RNA polymerase III
C	0046983	molecular_function	protein dimerization activity
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0001054	molecular_function	RNA polymerase I activity
E	0001055	molecular_function	RNA polymerase II activity
E	0001056	molecular_function	RNA polymerase III activity
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005665	cellular_component	RNA polymerase II, core complex
E	0005666	cellular_component	RNA polymerase III complex
E	0005730	cellular_component	nucleolus
E	0005736	cellular_component	RNA polymerase I complex
E	0005829	cellular_component	cytosol
E	0006351	biological_process	DNA-templated transcription
E	0006362	biological_process	transcription elongation by RNA polymerase I
E	0006366	biological_process	transcription by RNA polymerase II
E	0042797	biological_process	tRNA transcription by RNA polymerase III
E	0050821	biological_process	protein stabilization
E	1990062	cellular_component	RPAP3/R2TP/prefoldin-like complex
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0001054	molecular_function	RNA polymerase I activity
F	0001055	molecular_function	RNA polymerase II activity
F	0001056	molecular_function	RNA polymerase III activity
F	0001650	cellular_component	fibrillar center
F	0003677	molecular_function	DNA binding
F	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005665	cellular_component	RNA polymerase II, core complex
F	0005666	cellular_component	RNA polymerase III complex
F	0005730	cellular_component	nucleolus
F	0005736	cellular_component	RNA polymerase I complex
F	0005829	cellular_component	cytosol
F	0006351	biological_process	DNA-templated transcription
F	0006360	biological_process	transcription by RNA polymerase I
F	0006366	biological_process	transcription by RNA polymerase II
F	0042797	biological_process	tRNA transcription by RNA polymerase III
G	0000428	cellular_component	DNA-directed RNA polymerase complex
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005730	cellular_component	nucleolus
G	0005736	cellular_component	RNA polymerase I complex
G	0006351	biological_process	DNA-templated transcription
G	0006352	biological_process	DNA-templated transcription initiation
G	0006362	biological_process	transcription elongation by RNA polymerase I
G	1990830	biological_process	cellular response to leukemia inhibitory factor
H	0000428	cellular_component	DNA-directed RNA polymerase complex
H	0001054	molecular_function	RNA polymerase I activity
H	0001055	molecular_function	RNA polymerase II activity
H	0001056	molecular_function	RNA polymerase III activity
H	0003677	molecular_function	DNA binding
H	0003697	molecular_function	single-stranded DNA binding
H	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005665	cellular_component	RNA polymerase II, core complex
H	0005666	cellular_component	RNA polymerase III complex
H	0005730	cellular_component	nucleolus
H	0005736	cellular_component	RNA polymerase I complex
H	0005829	cellular_component	cytosol
H	0006351	biological_process	DNA-templated transcription
H	0006360	biological_process	transcription by RNA polymerase I
H	0006366	biological_process	transcription by RNA polymerase II
H	0006383	biological_process	transcription by RNA polymerase III
H	0032993	cellular_component	protein-DNA complex
I	0000428	cellular_component	DNA-directed RNA polymerase complex
I	0001054	molecular_function	RNA polymerase I activity
I	0003676	molecular_function	nucleic acid binding
I	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005730	cellular_component	nucleolus
I	0005736	cellular_component	RNA polymerase I complex
I	0006139	biological_process	nucleobase-containing compound metabolic process
I	0006351	biological_process	DNA-templated transcription
I	0006363	biological_process	termination of RNA polymerase I transcription
I	0008270	molecular_function	zinc ion binding
I	0046872	molecular_function	metal ion binding
J	0000428	cellular_component	DNA-directed RNA polymerase complex
J	0001054	molecular_function	RNA polymerase I activity
J	0001055	molecular_function	RNA polymerase II activity
J	0001056	molecular_function	RNA polymerase III activity
J	0003677	molecular_function	DNA binding
J	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005665	cellular_component	RNA polymerase II, core complex
J	0005666	cellular_component	RNA polymerase III complex
J	0005730	cellular_component	nucleolus
J	0005736	cellular_component	RNA polymerase I complex
J	0005829	cellular_component	cytosol
J	0006351	biological_process	DNA-templated transcription
J	0006356	biological_process	regulation of transcription by RNA polymerase I
J	0006360	biological_process	transcription by RNA polymerase I
J	0006366	biological_process	transcription by RNA polymerase II
J	0008270	molecular_function	zinc ion binding
J	0042797	biological_process	tRNA transcription by RNA polymerase III
J	0046872	molecular_function	metal ion binding
K	0000428	cellular_component	DNA-directed RNA polymerase complex
K	0001054	molecular_function	RNA polymerase I activity
K	0001056	molecular_function	RNA polymerase III activity
K	0003677	molecular_function	DNA binding
K	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005666	cellular_component	RNA polymerase III complex
K	0005730	cellular_component	nucleolus
K	0005736	cellular_component	RNA polymerase I complex
K	0006351	biological_process	DNA-templated transcription
K	0006362	biological_process	transcription elongation by RNA polymerase I
K	0006383	biological_process	transcription by RNA polymerase III
K	0031981	cellular_component	nuclear lumen
K	0046983	molecular_function	protein dimerization activity
K	0055029	cellular_component	nuclear DNA-directed RNA polymerase complex
L	0000428	cellular_component	DNA-directed RNA polymerase complex
L	0001054	molecular_function	RNA polymerase I activity
L	0001055	molecular_function	RNA polymerase II activity
L	0001056	molecular_function	RNA polymerase III activity
L	0003677	molecular_function	DNA binding
L	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005665	cellular_component	RNA polymerase II, core complex
L	0005666	cellular_component	RNA polymerase III complex
L	0005730	cellular_component	nucleolus
L	0005736	cellular_component	RNA polymerase I complex
L	0005829	cellular_component	cytosol
L	0006351	biological_process	DNA-templated transcription
L	0006356	biological_process	regulation of transcription by RNA polymerase I
L	0006360	biological_process	transcription by RNA polymerase I
L	0006366	biological_process	transcription by RNA polymerase II
L	0006383	biological_process	transcription by RNA polymerase III
L	0008270	molecular_function	zinc ion binding
L	0046872	molecular_function	metal ion binding
M	0000428	cellular_component	DNA-directed RNA polymerase complex
M	0001179	molecular_function	RNA polymerase I general transcription initiation factor binding
M	0001188	biological_process	RNA polymerase I preinitiation complex assembly
M	0001650	cellular_component	fibrillar center
M	0003677	molecular_function	DNA binding
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005730	cellular_component	nucleolus
M	0005736	cellular_component	RNA polymerase I complex
M	0006351	biological_process	DNA-templated transcription
M	0006361	biological_process	transcription initiation at RNA polymerase I promoter
M	0006362	biological_process	transcription elongation by RNA polymerase I
M	0042790	biological_process	nucleolar large rRNA transcription by RNA polymerase I
N	0000120	cellular_component	RNA polymerase I transcription regulator complex
N	0000428	cellular_component	DNA-directed RNA polymerase complex
N	0001650	cellular_component	fibrillar center
N	0003723	molecular_function	RNA binding
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005694	cellular_component	chromosome
N	0005730	cellular_component	nucleolus
N	0005736	cellular_component	RNA polymerase I complex
N	0005739	cellular_component	mitochondrion
N	0005829	cellular_component	cytosol
N	0006360	biological_process	transcription by RNA polymerase I
N	0006361	biological_process	transcription initiation at RNA polymerase I promoter
N	0007169	biological_process	cell surface receptor protein tyrosine kinase signaling pathway
N	0009303	biological_process	rRNA transcription

Functional Information from PROSITE/UniProt

site_id	PS00446
Number of Residues	41
Details	RNA_POL_D_30KD RNA polymerases D / 30 to 40 Kd subunits signature. NAFRRillaevptmAvekVlvynNtSivqDEiLAhRLGLIP

Chain	Residue	Details
C	ASN74-PRO114

---

site_id	PS00466
Number of Residues	36
Details	ZF_TFIIS_1 Zinc finger TFIIS-type signature. CprCghegmayhtRQMRSaDEGqtvfytCtn...CkfqE

Chain	Residue	Details
I	CYS87-GLU122

---

site_id	PS01030
Number of Residues	23
Details	RNA_POL_M_15KD RNA polymerases M / 15 Kd subunits signature. FCSDCGSVLplpgaqdtvt....CirC

Chain	Residue	Details
I	PHE19-CYS41

---

site_id	PS01110
Number of Residues	14
Details	RNA_POL_H_23KD RNA polymerases H / 23 Kd subunits signature. HELVPEHvvMtkEE

Chain	Residue	Details
E	HIS142-GLU155

---

site_id	PS01111
Number of Residues	15
Details	RNA_POL_K_14KD RNA polymerases K / 14 to 18 Kd subunits signature. TkYErARvLGtRAlQ

Chain	Residue	Details
F	THR58-GLN72

---

site_id	PS01112
Number of Residues	10
Details	RNA_POL_N_8KD RNA polymerases N / 8 Kd subunits signature. IIPVrCFTCG

Chain	Residue	Details
J	ILE2-GLY11

---

site_id	PS01154
Number of Residues	32
Details	RNA_POL_L_13KD RNA polymerases L / 13 to 16 Kd subunits signature. LheEdHTLgNsLrymImknpeVefcgYtttHP

Chain	Residue	Details
K	LEU44-PRO75

---

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKFASrHGQKGI

Chain	Residue	Details
B	GLY880-ILE892

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
M	SER35
J	CYS10
J	CYS44
A	ARG436

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692

Chain	Residue	Details
M	SER163
N	SER205
B	CYS1070
B	CYS1073
B	CYS1098
B	CYS1101
A	ARG1249

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:24207024

Chain	Residue	Details
M	LYS373
I	CYS23
I	CYS38
I	CYS41

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
N	SER128
I	CYS90
I	CYS115
I	CYS118

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
N	SER136

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
N	SER172
N	SER285

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
N	THR287

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17525332

Chain	Residue	Details
N	SER309

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
N	SER490

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
N	LYS270

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25218447, ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733

Chain	Residue	Details
N	LYS314

---