7OAT

Structural basis for targeted p97 remodelling by ASPL as prerequisite for p97 trimethylation by METTL21D

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Tether containing UBX domain for GLUT4	polymer	190	21606.6	1	UniProt (Q9BZE9) Pfam (PF00789)	Homo sapiens (Human)	Alveolar soft part sarcoma chromosomal region candidate gene 1 protein,Alveolar soft part sarcoma locus,Renal papillary cell carcinoma protein 17,UBX domain-containing protein 9
2	B (B)	Transitional endoplasmic reticulum ATPase	polymer	481	53506.1	1	UniProt (P55072) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862)	Homo sapiens (Human)	TER ATPase,15S Mg(2+)-ATPase p97 subunit,Valosin-containing protein,VCP
3	C (C)	Protein-lysine methyltransferase METTL21D	polymer	224	25232.9	1	UniProt (Q9H867) Pfam (PF10294)	Homo sapiens (Human)	Methyltransferase-like protein 21D,VCP lysine methyltransferase,VCP-KMT,Valosin-containing protein lysine methyltransferase
4	D, E, H, J (B, C)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5	F (B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
6	G (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	I (C)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
8	K, L, M (A, B, C)	water	water		18.0	58	Chemie (HOH)

Sequence modifications

A: 313 - 500 (UniProt: Q9BZE9)

PDB	External Database	Details
Gly 311	-	expression tag
Ser 312	-	expression tag

B: 2 - 480 (UniProt: P55072)

PDB	External Database	Details
Gly 0	-	expression tag
Ser 1	-	expression tag

C: 7 - 229 (UniProt: Q9H867)

PDB	External Database	Details
Gly 6	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	100345.6
Non-Polymers*	Number of molecules	7
	Total formula weight	1164.2
All*	Total formula weight	101509.8

*Water molecules are not included.