7NWI

Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	L8	polymer	244	26570.1	1	UniProt (G1TT27) Pfam (PF00181) Pfam (PF03947) In PDB	Oryctolagus cuniculus (Rabbit)
2	B	uL3	polymer	403	46108.0	1	UniProt (G1TL06) Pfam (PF00297) In PDB	Oryctolagus cuniculus (Rabbit)
3	C	60S ribosomal protein L4	polymer	425	47727.6	1		Oryctolagus cuniculus (Rabbit)
4	D	60S ribosomal protein L5	polymer	297	34481.8	1	UniProt (G1SYJ6) UniProt (by SIFTS) (P19949) In PDB	Oryctolagus cuniculus (Rabbit)
5	E	60S ribosomal protein L6	polymer	291	33055.3	1	UniProt (G1SKF7) Pfam (PF03868) Pfam (PF01159) In PDB	Oryctolagus cuniculus (Rabbit)
6	F	uL30	polymer	249	29518.3	1	UniProt (G1SV32) Pfam (PF08079) Pfam (PF00327) In PDB	Oryctolagus cuniculus (Rabbit)
7	G	60S ribosomal protein L7a	polymer	241	27480.6	1	UniProt (G1STW0) Pfam (PF01248) In PDB	Oryctolagus cuniculus (Rabbit)
8	H	L9	polymer	190	21627.2	1	UniProt (G1SWI6) Pfam (PF00347) In PDB	Oryctolagus cuniculus (Rabbit)
9	I	60S ribosomal protein L10	polymer	214	24643.1	1	UniProt (B7NZQ2) Pfam (PF00252) In PDB	Oryctolagus cuniculus (Rabbit)	Ribosomal protein L10 (Predicted)
10	J	Ribosomal protein L11	polymer	181	20670.9	1		Oryctolagus cuniculus (Rabbit)
11	L	L13	polymer	211	24347.7	1	UniProt (G1TKB3) Pfam (PF01294) In PDB	Oryctolagus cuniculus (Rabbit)
12	M	Ribosomal protein L14	polymer	218	23870.5	1	UniProt (G1SZ12) Pfam (PF01929) In PDB	Oryctolagus cuniculus (Rabbit)
13	N	Ribosomal protein L15	polymer	203	24076.1	1	UniProt (G1T0C1) Pfam (PF00827) In PDB	Oryctolagus cuniculus (Rabbit)
14	O	L13a	polymer	199	23248.9	1		Oryctolagus cuniculus (Rabbit)
15	P	L17	polymer	153	17757.6	1		Oryctolagus cuniculus (Rabbit)
16	Q	Ribosomal_L18e/L15P domain-containing protein	polymer	188	21588.6	1	UniProt (F6QKI9) Pfam (PF17135) In PDB	Oryctolagus cuniculus (Rabbit)
17	R	60S ribosomal protein L19	polymer	196	23535.3	1	UniProt (Q3T0W9) Pfam (PF01280) In PDB	Oryctolagus cuniculus (Rabbit)
18	S	L18a	polymer	175	20696.4	1		Oryctolagus cuniculus (Rabbit)
19	T	eL21	polymer	159	18478.8	1	UniProt (G1SHQ2) Pfam (PF01157) In PDB	Oryctolagus cuniculus (Rabbit)
20	U	60S ribosomal protein L22	polymer	128	14785.0	1	UniProt (Q4R5I3) Pfam (PF01776) In PDB	Oryctolagus cuniculus (Rabbit)
21	V	eL14	polymer	131	13972.4	1	UniProt (G1T6D1) Pfam (PF00238) In PDB	Oryctolagus cuniculus (Rabbit)
22	W	60S ribosomal protein L24-like protein	polymer	134	15538.3	1	UniProt (A0A061I3X8) Pfam (PF01246) In PDB	Oryctolagus cuniculus (Rabbit)
23	X	uL23	polymer	156	17768.2	1	UniProt (G1SE76) Pfam (PF03939) Pfam (PF00276) In PDB	Oryctolagus cuniculus (Rabbit)
24	Y	Ribosomal protein L26	polymer	134	15891.8	1	UniProt (G1SQH0) Pfam (PF16906) Pfam (PF00467) In PDB	Oryctolagus cuniculus (Rabbit)
25	Z	60S ribosomal protein L27	polymer	135	15704.6	1	UniProt (G1TXF6) Pfam (PF00467) Pfam (PF01777) In PDB	Oryctolagus cuniculus (Rabbit)
26	a	uL15	polymer	160	17831.9	1		Oryctolagus cuniculus (Rabbit)
27	b	60S ribosomal protein L29	polymer	245	26708.7	1		Oryctolagus cuniculus (Rabbit)
28	c	eL30	polymer	115	12805.1	1	UniProt (G1TDL2) Pfam (PF01248) In PDB	Oryctolagus cuniculus (Rabbit)
29	d	eL31	polymer	107	12635.7	1	UniProt (G1SHG0) Pfam (PF01198) In PDB	Oryctolagus cuniculus (Rabbit)
30	e	eL32	polymer	128	15022.0	1	UniProt (G1TUN8) Pfam (PF01655) In PDB	Oryctolagus cuniculus (Rabbit)
31	f	eL33	polymer	109	12449.6	1	UniProt (G1SF08) Pfam (PF01247) In PDB	Oryctolagus cuniculus (Rabbit)
32	g	60S ribosomal protein L34	polymer	126	14210.1	1	UniProt (G1U945) Pfam (PF01199) UniProt (by SIFTS) (G1TXG5) In PDB	Oryctolagus cuniculus (Rabbit)
33	h	uL29	polymer	122	14435.4	1	UniProt (G1SIT5) Pfam (PF00831) In PDB	Oryctolagus cuniculus (Rabbit)
34	i	60S ribosomal protein L36	polymer	117	13546.3	1		Oryctolagus cuniculus (Rabbit)
35	j	Ribosomal protein L37	polymer	97	11111.0	1	UniProt (U3KPD5) Pfam (PF01907) In PDB	Oryctolagus cuniculus (Rabbit)
36	k	L38	polymer	69	8107.8	1		Oryctolagus cuniculus (Rabbit)
37	l	ribosomal protein eL39	polymer	50	6295.6	1	UniProt (G1SYU7) Pfam (PF00832) In PDB	Oryctolagus cuniculus (Rabbit)
38	m	60S ribosomal protein L40	polymer	128	14695.3	1	UniProt (A0A6P4TG29) In PDB	Oryctolagus cuniculus (Rabbit)	CEP52,Ubiquitin,Ubiquitin A-52 residue ribosomal protein fusion product 1,Ubiquitin-60S ribosomal protein L40
39	n	60s ribosomal protein l41	polymer	23	3213.1	1	UniProt (A0A087WNH4) Pfam (PF05162) In PDB	Oryctolagus cuniculus (Rabbit)
40	o	LOW QUALITY PROTEIN: 60S ribosomal protein L36a	polymer	142	16170.2	1	UniProt (A0A6P5P305) Pfam (PF00935) In PDB	Oryctolagus cuniculus (Rabbit)
41	p	ribosomal protein eL43	polymer	91	10168.2	1	UniProt (G1SY53) Pfam (PF01780) In PDB	Oryctolagus cuniculus (Rabbit)
42	r	eL28	polymer	137	15783.6	1	UniProt (G1U7L1) Pfam (PF01778) In PDB	Oryctolagus cuniculus (Rabbit)
43	s	60S acidic ribosomal protein P0	polymer	318	34380.5	1	UniProt (G1SPK4) Pfam (PF00466) Pfam (PF17777) Pfam (PF00428) In PDB	Oryctolagus cuniculus (Rabbit)
44	t	L12	polymer	163	17689.5	1	UniProt (G1SMR7) Pfam (PF03946) Pfam (PF00298) In PDB	Oryctolagus cuniculus (Rabbit)
45	AA	40S ribosomal protein SA	polymer	295	32928.0	1	UniProt (G1TLT8) Pfam (PF00318) Pfam (PF16122) In PDB	Oryctolagus cuniculus (Rabbit)	37 kDa laminin receptor precursor,37LRP,37/67 kDa laminin receptor,LRP/LR,67 kDa laminin receptor,67LR,Laminin receptor 1,LamR,Laminin-binding protein precursor p40,LBP/p40
46	BB	40S ribosomal protein S3a	polymer	264	29942.0	1	UniProt (P97351) Pfam (PF01015) In PDB	Oryctolagus cuniculus (Rabbit)	Protein TU-11
47	CC	40S ribosomal protein S2	polymer	259	27956.6	1	UniProt (O55214) Pfam (PF00333) Pfam (PF03719) In PDB	Oryctolagus cuniculus (Rabbit)
48	DD	40S ribosomal protein S3	polymer	281	31146.6	1	UniProt (G1TNM3) Pfam (PF07650) Pfam (PF00189) In PDB	Oryctolagus cuniculus (Rabbit)
49	EE	S4	polymer	262	29497.7	1		Oryctolagus cuniculus (Rabbit)
50	FF	Ribosomal protein S5	polymer	205	23044.7	1	UniProt (G1TFM5) Pfam (PF00177) In PDB	Oryctolagus cuniculus (Rabbit)
51	GG	40S ribosomal protein S6	polymer	262	30070.4	1	UniProt (A0A6G1APX9) Pfam (PF01092) In PDB	Oryctolagus cuniculus (Rabbit)
52	HH	ribosomal protein eS7	polymer	190	21716.4	1	UniProt (G1SVB0) In PDB	Oryctolagus cuniculus (Rabbit)
53	II	40S ribosomal protein S8	polymer	206	24003.0	1	UniProt (G1TJW1) Pfam (PF01201) In PDB	Oryctolagus cuniculus (Rabbit)
54	JJ	40S ribosomal protein S9	polymer	194	22655.6	1	UniProt (A0A1S3APZ1) Pfam (PF00163) Pfam (PF01479) In PDB	Oryctolagus cuniculus (Rabbit)
55	KK	S10_plectin domain-containing protein	polymer	98	11774.0	1	UniProt (G1T168) Pfam (PF03501) In PDB	Oryctolagus cuniculus (Rabbit)
56	LL	40S ribosomal protein S11	polymer	158	18468.8	1	UniProt (G1TRM4) Pfam (PF16205) Pfam (PF00366) In PDB	Oryctolagus cuniculus (Rabbit)
57	MM	40S ribosomal protein S12	polymer	124	13766.1	1	UniProt (G1SFR8) Pfam (PF01248) In PDB	Oryctolagus cuniculus (Rabbit)
58	NN	ribosomal protein uS15	polymer	150	17128.2	1	UniProt (G1SP51) Pfam (PF08069) Pfam (PF00312) In PDB	Oryctolagus cuniculus (Rabbit)
59	OO	S14	polymer	168	18134.0	1		Oryctolagus cuniculus (Rabbit)
60	PP	40S ribosomal protein uS19	polymer	145	17049.2	1	UniProt (G1U0Q2) Pfam (PF00203) In PDB	Oryctolagus cuniculus (Rabbit)
61	QQ	Rps16 protein	polymer	157	17585.7	1	UniProt (Q5CZY9) Pfam (PF00380) In PDB	Oryctolagus cuniculus (Rabbit)
62	RR	S17	polymer	129	14969.4	1	UniProt (G1TU13) Pfam (PF00833) In PDB	Oryctolagus cuniculus (Rabbit)
63	SS	ribosomal protein uS13	polymer	137	16170.8	1	UniProt (G1TPG3) Pfam (PF00416) In PDB	Oryctolagus cuniculus (Rabbit)
64	TT	S19	polymer	141	15683.1	1		Oryctolagus cuniculus (Rabbit)
65	UU	Ribosomal_S10 domain-containing protein	polymer	104	11765.9	1	UniProt (A0A5F9CX53) Pfam (PF00338) In PDB	Oryctolagus cuniculus (Rabbit)
66	VV	S21	polymer	83	9115.4	1		Oryctolagus cuniculus (Rabbit)
67	WW	Ribosomal protein S15a	polymer	139	15778.6	1		Oryctolagus cuniculus (Rabbit)
68	XX	Ribosomal protein S23	polymer	141	15626.4	1	UniProt (G1SZ47) Pfam (PF00164) In PDB	Oryctolagus cuniculus (Rabbit)
69	YY	40S ribosomal protein S24	polymer	146	17007.1	1		Oryctolagus cuniculus (Rabbit)
70	ZZ	40S ribosomal protein S25	polymer	123	13582.0	1	UniProt (G1TDB3) Pfam (PF03297) In PDB	Oryctolagus cuniculus (Rabbit)
71	aa	40S ribosomal protein S26	polymer	117	13147.6	1	UniProt (T0M5X4) Pfam (PF01283) In PDB	Oryctolagus cuniculus (Rabbit)
72	bb	40S ribosomal protein S27	polymer	84	9480.2	1	UniProt (G1TZ76) Pfam (PF01667) In PDB	Oryctolagus cuniculus (Rabbit)
73	cc	40S ribosomal protein S28	polymer	69	7776.9	1	UniProt (G1TIB4) Pfam (PF01200) In PDB	Oryctolagus cuniculus (Rabbit)
74	dd	ribosomal protein uS14	polymer	53	6364.4	1	UniProt (G1U7M4) Pfam (PF00253) In PDB	Oryctolagus cuniculus (Rabbit)
75	ee	40S ribosomal protein S30	polymer	133	14498.9	1	UniProt (G1T8A2) Pfam (PF00240) Pfam (PF04758) In PDB	Oryctolagus cuniculus (Rabbit)
76	ff	S27a	polymer	62	7468.9	1		Oryctolagus cuniculus (Rabbit)
77	gg	ribosomal protein RACK1	polymer	313	34669.1	1	UniProt (G1SJB4) Pfam (PF00400) In PDB	Oryctolagus cuniculus (Rabbit)
78	1	Sec61Beta	polymer	15	1788.0	1		Oryctolagus cuniculus (Rabbit)
79	2	P-Site tRNA	polymer	76	24436.6	1		Oryctolagus cuniculus (Rabbit)
80	5	28S ribosomal RNA+BlaS	polymer	3788	1226739.5	1		Oryctolagus cuniculus (Rabbit)
81	7	5S ribosomal RNA	polymer	120	38691.9	1	GenBank (4CXE_4)	Oryctolagus cuniculus (Rabbit)
82	8	5.8S ribosomal RNA	polymer	156	50143.6	1	GenBank (4CXE_3)	Oryctolagus cuniculus (Rabbit)
83	9	18S ribosomal RNA	polymer	1777	573221.6	1		Oryctolagus cuniculus (Rabbit)
84	K	mRNA	polymer	10	3226.0	1		Oryctolagus cuniculus (Rabbit)
85	5, 8, 9, B, P...	MAGNESIUM ION	non-polymer		24.3	210	Chemie (MG)
86	g, j, m, o, p	ZINC ION	non-polymer		65.4	5	Chemie (ZN)
87	5	BLASTICIDIN S	non-polymer		422.4	1	Chemie (BLS)

Sequence modifications

B: 2 - 403 (UniProt: G1TL06)

PDB	External Database	Details
Met 1	-	initiating methionine

D: 2 - 297 (UniProt: G1SYJ6)

PDB	External Database	Details
Met 1	-	initiating methionine

E: -5 - 284 (UniProt: G1SKF7)

PDB	External Database	Details
Arg 126	Lys 133	conflict
Gln 217	Lys 224	conflict

F: 2 - 250 (UniProt: G1SV32)

PDB	External Database	Details
Ala 175	Thr 174	conflict
Gly 185	Asn 184	conflict
Arg 202	His 201	conflict
Glu 233	Gly 232	conflict

G: 26 - 266 (UniProt: G1STW0)

PDB	External Database	Details
Gly 191	Cys 167	conflict

L: 1 - 211 (UniProt: G1TKB3)

PDB	External Database	Details
Ser 52	Ala 52	conflict
Leu 55	Ile 55	conflict
Arg 74	His 74	conflict
Arg 190	His 190	conflict

Q: 1 - 188 (UniProt: F6QKI9)

PDB	External Database	Details
Gly 77	Asn 77	conflict
Ile 86	Val 86	conflict
Ser 106	Thr 106	conflict

c: 1 - 115 (UniProt: G1TDL2)

PDB	External Database	Details
Thr 34	Ser 34	conflict
Ala 95	Ser 95	conflict

m: 1 - 128 (UniProt: A0A6P4TG29)

PDB	External Database	Details
Leu 54	Arg 54	conflict
His 74	Arg 74	conflict

CC: 35 - 293 (UniProt: O55214)

PDB	External Database	Details
Leu 61	Ile 27	conflict
Val 73	Met 39	conflict
Ala 101	Ser 67	conflict
Ala 119	Gly 85	conflict
Lys 142	Glu 108	conflict
Leu 215	Met 181	conflict
Trp 227	Arg 193	conflict

FF: 1 - 204 (UniProt: G1TFM5)

PDB	External Database	Details
Met 0	-	initiating methionine

II: 2 - 207 (UniProt: G1TJW1)

PDB	External Database	Details
Arg 47	Gly 47	variant

ZZ: 4 - 125 (UniProt: G1TDB3)

PDB	External Database	Details
Met 3	-	initiating methionine

cc: 1 - 69 (UniProt: G1TIB4)

PDB	External Database	Details
His 5	Arg 5	conflict
Ile 18	Leu 18	conflict
Lys 20	Arg 20	conflict
His 40	Arg 40	conflict
Thr 42	Ile 42	conflict

Contents of the asymmetric unit

Polymers	Number of chains	84
	Total formula weight	3408621.4
Non-Polymers*	Number of molecules	216
	Total formula weight	5853.5
All*	Total formula weight	3414474.9

*Water molecules are not included.