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- EMDB-12633: Mammalian pre-termination 80S ribosome with Empty-A site bound by... -

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Basic information

Entry
Database: EMDB / ID: EMD-12633
TitleMammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S
Map data
Sample
  • Complex: Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S
    • Protein or peptide: x 69 types
    • RNA: x 1 types
  • Protein or peptide: x 9 types
  • RNA: x 5 types
  • Ligand: x 3 types
Function / homology
Function and homology information


Formation of the ternary complex, and subsequently, the 43S complex / Formation of a pool of free 40S subunits / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Translation initiation complex formation / Ribosomal scanning and start codon recognition / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit ...Formation of the ternary complex, and subsequently, the 43S complex / Formation of a pool of free 40S subunits / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Translation initiation complex formation / Ribosomal scanning and start codon recognition / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit / Major pathway of rRNA processing in the nucleolus and cytosol / GTP hydrolysis and joining of the 60S ribosomal subunit / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / rough endoplasmic reticulum / translation initiation factor binding / DNA-(apurinic or apyrimidinic site) lyase / small-subunit processome / cytosolic ribosome / mRNA 5'-UTR binding / ribosomal small subunit biogenesis / cytosolic small ribosomal subunit / heparin binding / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / postsynapse / cell differentiation / rRNA binding / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / synapse / nucleolus / negative regulation of apoptotic process / endoplasmic reticulum / RNA binding / nucleus / cytosol / cytoplasm
Similarity search - Function
: / metallochaperone-like domain / TRASH domain / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S26e signature. / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. ...: / metallochaperone-like domain / TRASH domain / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein S26e signature. / Ribosomal protein S3Ae, conserved site / Ribosomal protein S12e signature. / Ribosomal protein S5, eukaryotic/archaeal / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S6, eukaryotic / Ribosomal protein L24e, conserved site / Ribosomal protein L44e / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S3Ae / Ribosomal S3Ae family / Ribosomal protein L44 / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein L19, eukaryotic / Ribosomal S3Ae family / Ribosomal protein S17e signature. / Ribosomal L40e family / Ribosomal protein L44e signature. / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein L27e signature. / Ribosomal protein L10e signature. / Ribosomal protein S7e signature. / Ribosomal protein L19/L19e conserved site / Ribosomal protein S6e / Ribosomal protein S3Ae signature. / Ribosomal protein S27e signature. / Ribosomal protein S8e signature. / Ribosomal protein L19e signature. / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L34e signature. / Ribosomal protein L6e signature. / Ribosomal protein L30e signature 1. / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / 60S ribosomal protein L19 / Ribosomal protein L39e signature. / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein S6e signature. / Ribosomal protein L35Ae signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal protein L18e / Ribosomal protein S28e signature. / Ribosomal protein L24e / Ribosomal protein L31e signature. / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L32e signature. / Ribosomal protein L6 signature 2. / Ribosomal protein L15e signature. / Ribosomal protein L21e signature. / Ribosomal protein L37e signature. / Ribosomal protein L11 signature. / Ubiquitin conserved site / Ubiquitin domain / Ribosomal protein S2 signature 2. / Ubiquitin domain signature. / Ribosomal protein L23 signature. / Ribosomal protein S10, conserved site / Ribosomal protein L30 signature. / Ribosomal protein S10 / Ribosomal protein S3 signature. / Ribosomal protein S10 signature. / Ribosomal protein S14 signature. / Ubiquitin family / Ribosomal protein S2 signature 1. / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4/S9, N-terminal / Ribosomal protein S4, conserved site / Ribosomal protein L29 signature. / Type-2 KH domain profile. / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4/S9 / Ubiquitin homologues / Ribosomal protein S19 signature. / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S5
Similarity search - Domain/homology
Large ribosomal subunit protein eL24 / Small ribosomal subunit protein eS32 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS10 / 40S ribosomal protein S6 / 60S ribosomal protein L40 / LOW QUALITY PROTEIN: 60S ribosomal protein L36a / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Large ribosomal subunit protein uL23 ...Large ribosomal subunit protein eL24 / Small ribosomal subunit protein eS32 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS10 / 40S ribosomal protein S6 / 60S ribosomal protein L40 / LOW QUALITY PROTEIN: 60S ribosomal protein L36a / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Small ribosomal subunit protein eS12 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL11 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL30 / Small ribosomal subunit protein eS7 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL14 / Small ribosomal subunit protein uS12 / Large ribosomal subunit protein eL15 / Small ribosomal subunit protein eS10 / Large ribosomal subunit protein uL14 / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Large ribosomal subunit protein eL30 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL2 / Small ribosomal subunit protein eS17 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL27 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein eL28 / Small ribosomal subunit protein uS14 / Large ribosomal subunit protein eL34 / 40S ribosomal protein S2 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein eL19 / Large ribosomal subunit protein eL22 / Rps16 protein / 40S ribosomal protein S26 / Large ribosomal subunit protein eL37
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit) / Rabbit (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.13 Å
AuthorsPowers KT / Yadav SKN / Bufton JC / Schaffitzel C
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Wellcome Trust210701/Z/18/Z United Kingdom
CitationJournal: Nucleic Acids Res / Year: 2021
Title: Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA.
Authors: Kyle T Powers / Flint Stevenson-Jones / Sathish K N Yadav / Beate Amthor / Joshua C Bufton / Ufuk Borucu / Dakang Shen / Jonas P Becker / Daria Lavysh / Matthias W Hentze / Andreas E Kulozik ...Authors: Kyle T Powers / Flint Stevenson-Jones / Sathish K N Yadav / Beate Amthor / Joshua C Bufton / Ufuk Borucu / Dakang Shen / Jonas P Becker / Daria Lavysh / Matthias W Hentze / Andreas E Kulozik / Gabriele Neu-Yilik / Christiane Schaffitzel /
Abstract: Deciphering translation is of paramount importance for the understanding of many diseases, and antibiotics played a pivotal role in this endeavour. Blasticidin S (BlaS) targets translation by binding ...Deciphering translation is of paramount importance for the understanding of many diseases, and antibiotics played a pivotal role in this endeavour. Blasticidin S (BlaS) targets translation by binding to the peptidyl transferase center of the large ribosomal subunit. Using biochemical, structural and cellular approaches, we show here that BlaS inhibits both translation elongation and termination in Mammalia. Bound to mammalian terminating ribosomes, BlaS distorts the 3'CCA tail of the P-site tRNA to a larger extent than previously reported for bacterial ribosomes, thus delaying both, peptide bond formation and peptidyl-tRNA hydrolysis. While BlaS does not inhibit stop codon recognition by the eukaryotic release factor 1 (eRF1), it interferes with eRF1's accommodation into the peptidyl transferase center and subsequent peptide release. In human cells, BlaS inhibits nonsense-mediated mRNA decay and, at subinhibitory concentrations, modulates translation dynamics at premature termination codons leading to enhanced protein production.
History
DepositionMar 16, 2021-
Header (metadata) releaseJul 7, 2021-
Map releaseJul 7, 2021-
UpdateJan 11, 2023-
Current statusJan 11, 2023Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.014
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.014
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-7nwi
  • Surface level: 0.015
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_12633.png

Downloads & links

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Map

FileDownload / File: emd_12633.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.35 Å
Density
Contour LevelBy AUTHOR: 0.014 / Movie #1: 0.014
Minimum - Maximum-0.078272074 - 0.17347491
Average (Standard dev.)0.0010667698 (±0.006694124)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 405.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.351.351.35
M x/y/z300300300
origin x/y/z0.0000.0000.000
length x/y/z405.000405.000405.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS300300300
D min/max/mean-0.0780.1730.001

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Supplemental data

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Mask #1

Fileemd_12633_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_12633_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_12633_half_map_2.map
Projections & Slices
AxesZYX

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Histogram (log scale)

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Sample components

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Entire : Mammalian pre-termination 80S ribosome with Empty-A site bound by...

EntireName: Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S
Components
  • Complex: Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S
    • Protein or peptide: L8
    • Protein or peptide: uL3
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: uL30
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: L9
    • Protein or peptide: Ribosomal protein L11
    • Protein or peptide: L13
    • Protein or peptide: Ribosomal protein L14
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: L13a
    • Protein or peptide: Ribosomal_L18e/L15P domain-containing protein
    • Protein or peptide: 60S ribosomal protein L19
    • Protein or peptide: L18a
    • Protein or peptide: eL21
    • Protein or peptide: 60S ribosomal protein L22
    • Protein or peptide: 60S ribosomal protein L24-like proteinRibosome
    • Protein or peptide: uL23
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: uL15
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: eL30
    • Protein or peptide: eL31
    • Protein or peptide: eL32CD59
    • Protein or peptide: eL33
    • Protein or peptide: uL29
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: L38
    • Protein or peptide: ribosomal protein eL39
    • Protein or peptide: 60s ribosomal protein l41
    • Protein or peptide: eL28
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: L12
    • Protein or peptide: 40S ribosomal protein SA
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: 40S ribosomal protein S2
    • Protein or peptide: 40S ribosomal protein S3
    • Protein or peptide: S4
    • Protein or peptide: Ribosomal protein S5
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: ribosomal protein eS7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: S10_plectin domain-containing protein
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: ribosomal protein uS15
    • Protein or peptide: S14
    • Protein or peptide: 40S ribosomal protein uS19
    • Protein or peptide: Rps16 protein40S ribosomal protein S16
    • Protein or peptide: S17
    • Protein or peptide: ribosomal protein uS13
    • Protein or peptide: S19
    • Protein or peptide: Ribosomal_S10 domain-containing protein
    • Protein or peptide: S21
    • Protein or peptide: Ribosomal protein S15aRibosome
    • Protein or peptide: Ribosomal protein S23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S28
    • Protein or peptide: ribosomal protein uS14
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: S27a
    • Protein or peptide: ribosomal protein RACK1
    • Protein or peptide: Sec61Beta
    • RNA: P-Site tRNA
  • Protein or peptide: 60S ribosomal protein L4
  • Protein or peptide: 60S ribosomal protein L10
  • Protein or peptide: L17
  • Protein or peptide: eL14
  • Protein or peptide: 60S ribosomal protein L34
  • Protein or peptide: Ribosomal protein L37
  • Protein or peptide: 60S ribosomal protein L40
  • Protein or peptide: LOW QUALITY PROTEIN: 60S ribosomal protein L36a
  • Protein or peptide: ribosomal protein eL43
  • RNA: 28S ribosomal RNA+BlaS
  • RNA: 5S ribosomal RNA
  • RNA: 5.8S ribosomal RNA
  • RNA: 18S ribosomal RNA
  • RNA: mRNAMessenger RNA
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION
  • Ligand: BLASTICIDIN S

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Supramolecule #1: Mammalian pre-termination 80S ribosome with Empty-A site bound by...

SupramoleculeName: Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S
type: complex / ID: 1 / Parent: 0
Macromolecule list: #1-#2, #4-#8, #10-#14, #16-#20, #22-#31, #33-#34, #36-#37, #39, #42-#79
Details: Rabbit reticulocyte lysate derived (in vitro transcription-FLAG affinity purified) ribosomal complex.
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 3.8 MDa

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Macromolecule #1: L8

MacromoleculeName: L8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 26.570105 KDa
SequenceString: GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV ...String:
GRVIRGQRKG AGSVFRAHVK HRKGAARLRA VDFAERHGYI KGIVKDIIHD PGRGAPLAKV VFRDPYRFKK RTELFIAAEG IHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS A NRAVVGVV AGGGRIDKPI LKAGRAYHKY KAKRNCWPRV RGVAMNPVEH PFGGGNHQHI GKPSTIRRDA PAGRKVGLIA AR RTGR

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Macromolecule #2: uL3

MacromoleculeName: uL3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 46.107977 KDa
SequenceString: MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM ...String:
MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVS QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKTHRG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINPL GGF VHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTVEEKK AFMGPLKKDR IAKE EGA

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Macromolecule #3: 60S ribosomal protein L4

MacromoleculeName: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 47.727559 KDa
SequenceString: MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV ...String:
MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC VIYNEDNGIV KAFRNIPGIT LLNVTKLNIL KL APGGHVG RFCIWTESAF RKLDDLYGTW RKAASLKSNY NLPMHKMLNT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVERAAA ALAAKSDPKE APAKKKPVVG KKVKKPRAVG IKQKKKPVVG RKAA AAKKP AADKKAADKR AGPEDKKPAA

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Macromolecule #4: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.481828 KDa
SequenceString: MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD ...String:
MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMMEE MY KKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

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Macromolecule #5: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 33.055297 KDa
SequenceString: MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGRKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL ...String:
MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGRKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKQLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

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Macromolecule #6: uL30

MacromoleculeName: uL30 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.518281 KDa
SequenceString: LFANNIREKK KVPAVPETLK KKRRNFAELK IKRLRKKFAQ KMLRKARRKL IYEKAKHYHK EYRQMYRTEI RMARMARKAG NFYVPAEPK LAFVIRIRGI NGVSPKVRKV LQLLRLRQIF NGTFVKLNKA SINMLRIVEP YIAWGYPNLK SVNELIYKRG Y GKINKKRI ...String:
LFANNIREKK KVPAVPETLK KKRRNFAELK IKRLRKKFAQ KMLRKARRKL IYEKAKHYHK EYRQMYRTEI RMARMARKAG NFYVPAEPK LAFVIRIRGI NGVSPKVRKV LQLLRLRQIF NGTFVKLNKA SINMLRIVEP YIAWGYPNLK SVNELIYKRG Y GKINKKRI ALTDNALIAR SLGKYGIICM EDLIHEIYTV GKRFKEANNF LWPFKLSSPR GGMKKKTTHF VEGEDAGNRE DQ INRLIRR MN

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Macromolecule #7: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 27.480555 KDa
SequenceString: KVVNPLFEKR PKNFGIGQDI QPKRDLTRFV KWPRYIRLQR QRAILYKRLK VPPAINQFTQ VLDRQTATQL LKLAHKYRPE TKQEKKQRL LARAEKKAAG KGDVPTKRPP VLRAGVNTVT TLVENKKAQL VVIAHDVDPI ELVVFLPALC RKMGVPYCIL K GKARLGRL ...String:
KVVNPLFEKR PKNFGIGQDI QPKRDLTRFV KWPRYIRLQR QRAILYKRLK VPPAINQFTQ VLDRQTATQL LKLAHKYRPE TKQEKKQRL LARAEKKAAG KGDVPTKRPP VLRAGVNTVT TLVENKKAQL VVIAHDVDPI ELVVFLPALC RKMGVPYCIL K GKARLGRL VHRKTCTTVA FTQVNSEDKG ALAKLVEAIR TNYNDRYDEI RRHWGGNVLG PKSVARIAKL EKAKAKELAT KL G

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Macromolecule #8: L9

MacromoleculeName: L9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.627215 KDa
SequenceString:
MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QA

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Macromolecule #9: 60S ribosomal protein L10

MacromoleculeName: 60S ribosomal protein L10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.643057 KDa
SequenceString: MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS ...String:
MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

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Macromolecule #10: Ribosomal protein L11

MacromoleculeName: Ribosomal protein L11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 20.670904 KDa
SequenceString:
MAQRPQDQGE KENPMRELRI RKLCLNICVG ESGDRLTRAA KVLEQLTGQT PVFSKARYTV RSFGIRRNEK IAVHCTVRGA KAEEILEKG LKVREYELRK NNFSDTGNFG FGIQEHIDLG IKYDPSIGIY GLDFYVVLGR PGFSIADKKR RTGCIGAKHR I SKEEAMRW FQQKYDGIIL PGK

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Macromolecule #11: L13

MacromoleculeName: L13 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.347723 KDa
SequenceString: MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP ASGPLRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ...String:
MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP ASGPLRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

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Macromolecule #12: Ribosomal protein L14

MacromoleculeName: Ribosomal protein L14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.870549 KDa
SequenceString: MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG ...String:
MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

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Macromolecule #13: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.076088 KDa
SequenceString: GAYKYIQELW RKKQSDVMRF LLRVRCWQYR QLSALHRAPR PTRPDKARRL GYKAKQGYVI YRIRVRRGGR KRPVPKGATY GKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE M RGLTSAGR ...String:
GAYKYIQELW RKKQSDVMRF LLRVRCWQYR QLSALHRAPR PTRPDKARRL GYKAKQGYVI YRIRVRRGGR KRPVPKGATY GKPVHHGVN QLKFARSLQS VAEERAGRHC GALRVLNSYW VGEDSTYKFF EVILIDPFHK AIRRNPDTQW ITKPVHKHRE M RGLTSAGR KSRGLGKGHK FHHTIGGSRR AAWRRRNTLQ LHRYR

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Macromolecule #14: L13a

MacromoleculeName: L13a / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.248924 KDa
SequenceString: QVLVLDGRGH LLGRLAAIVA KQVLLGRKVV VVRCEGINIS GNFYRNKLKY LAFLRKRMNT NPSRGPYHFR APSRIFWRTV RGMLPHKTK RGQAALDRLK VFDGIPPPYD KKKRMVVPAA LKVVRLKPTR KFAYLGRLAH EVGWKYQAVT ATLEEKRKEK A KIHYRKKK ...String:
QVLVLDGRGH LLGRLAAIVA KQVLLGRKVV VVRCEGINIS GNFYRNKLKY LAFLRKRMNT NPSRGPYHFR APSRIFWRTV RGMLPHKTK RGQAALDRLK VFDGIPPPYD KKKRMVVPAA LKVVRLKPTR KFAYLGRLAH EVGWKYQAVT ATLEEKRKEK A KIHYRKKK QLMRLRKQAE KNVEKKIDRF TEVLKTHGFL V

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Macromolecule #15: L17

MacromoleculeName: L17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.757627 KDa
SequenceString:
VRYSLDPENP TKSCKSRGSN LRVHFKNTRE TAQAIKGMHI RKATKYLKDV TLQKQCVPFR RYNGGVGRCA QAKQWGWTQG RWPKKSAEF LLHMLKNAES NAELKGLDVD SLVIEHIQVN KAPKMRRRTY RAHGRINPYM SSPCHIEMIL TEKE

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Macromolecule #16: Ribosomal_L18e/L15P domain-containing protein

MacromoleculeName: Ribosomal_L18e/L15P domain-containing protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.588586 KDa
SequenceString:
MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGREGKTA VVVGTITDD VRVQEVPKLK VCALRVSSRA RSRILKAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

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Macromolecule #17: 60S ribosomal protein L19

MacromoleculeName: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.535281 KDa
SequenceString: MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ...String:
MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

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Macromolecule #18: L18a

MacromoleculeName: L18a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 20.696363 KDa
SequenceString:
KASGTLREYK VVGRCLPTPK CRTPPLYRMR IFAPNHVVAK SRFWYFVSQL KKMKKSSGEI VYCGQVFEKS PLRVKNFGIW LRYDSRSGT HNMYREYRDL TTAGAVTQCY RDMGARHRAR AHSIQIMKVE EIAASKCRRP AVKQFHDSKI KFPLPHRVLR R QHKPRFTT KRPNTFF

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Macromolecule #19: eL21

MacromoleculeName: eL21 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.478791 KDa
SequenceString:
TNTKGKRRGT RYMFSRPFRK HGVVPLATYM RIYKKGDIVD IKGMGTVQKG MPHKCYHGKT GRVYNVTQHA VGIVVNKQVK GKILAKRIN VRIEHIKHSK SRDSFLKRVK ENDQKKKEAK EKGTWVQLKR QPAPPREAHF VRTNGKEPEL LEPIPYEFMA

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Macromolecule #20: 60S ribosomal protein L22

MacromoleculeName: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.784962 KDa
SequenceString:
MAPVKKLVAK GGKKKKQVLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VTIERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANSKES YELRYFQINQ DEEEEEDED

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Macromolecule #21: eL14

MacromoleculeName: eL14 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.972425 KDa
SequenceString:
SGAKFRISLG LPVGAVINCA DNTGAKNLYI ISVKGIKGRL NRLPAAGVGD MVMATVKKGK PELRKKVHPA VVIRQRKSYR RKDGVFLYF EDNAGVIVNN KGEMKGSAIT GPVAKECADL WPRIASNAGS IA

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Macromolecule #22: 60S ribosomal protein L24-like protein

MacromoleculeName: 60S ribosomal protein L24-like protein / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.538256 KDa
SequenceString:
MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKEKR

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Macromolecule #23: uL23

MacromoleculeName: uL23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.768246 KDa
SequenceString:
MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

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Macromolecule #24: Ribosomal protein L26

MacromoleculeName: Ribosomal protein L26 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.891787 KDa
SequenceString:
MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGK

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Macromolecule #25: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.704635 KDa
SequenceString:
GKFMKPGKVV LVLAGRYSGR KAVIVKNIDD GTSDRPYSHA LVAGIDRYPR KVTAAMGKKK IAKRSKIKSF VKVYNYNHLM PTRYSVDIP LDKTVVNKDV FRDPALKRKA RREAKVKFEE RYKTGKNKWF FQKLRF

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Macromolecule #26: uL15

MacromoleculeName: uL15 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.831941 KDa
SequenceString:
MVAAKCQHRG GAEPSRLRKT RKLRGHVSHG HGRIGKHRKH PGGRGNAGGM HHHRINFDKY HPGYFGKVGM RHYHLKRNQS FCPTVNLDK LWTLVSEQTR VNAAKNKTGA APIIDVVRSG YYKVLGKGKL PKQPVIVKAK FFSRRAEEKI KGVGGACVLV A

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Macromolecule #27: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 26.708707 KDa
SequenceString: MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE ...String:
MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPAAP VPAQAPPKGA QP PAKAP

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Macromolecule #28: eL30

MacromoleculeName: eL30 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.805092 KDa
SequenceString:
MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQTLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLAIIDP GDSDIIRSMP EQTGEK

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Macromolecule #29: eL31

MacromoleculeName: eL31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.635694 KDa
SequenceString:
NEVVTREYTI NIHKRIHGVG FKKRAPRALK EIRKFAMKEM GTPDVRIDTR LNKAVWAKGI RNVPYRIRVR LSRKRNEDED SPNKLYTLV TYVPVTTFKN LQTVNVDE

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Macromolecule #30: eL32

MacromoleculeName: eL32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.022021 KDa
SequenceString:
AALRPLVKPK IVKKRTKKFI RHQSDRYVKI KRNWRKPRGI DNRVRRRFKG QILMPNIGYG SNKKTKHMLP SGFRKFLVHN VKELEVLLM CNKSYCAEIA HNVSSKNRKA IVERAAQLAI RVTNPNARL

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Macromolecule #31: eL33

MacromoleculeName: eL33 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 12.449612 KDa
SequenceString:
SGRLWCKAIF AGYKRGLRNQ REHTALLKIE GVYARDETEF YLGKRCAYVY KAKNNTVTPG GKPNKTRVIW GKVTRAHGNS GMVRAKFRS NLPAKAIGHR IRVMLYPSRI

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Macromolecule #32: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.210088 KDa
SequenceString:
LPGRCLEAAT MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYG GSMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKA

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Macromolecule #33: uL29

MacromoleculeName: uL29 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.435403 KDa
SequenceString:
AKIKARDLRG KKKEELLKQL DDLKVELSQL RVAKVTGGAA SKLSKIRVVR KSIARVLTVI NQTQKENLRK FYKGKKYKPL DLRPKKTRA MRRRLNKHEE SLKTKKQQRK ERLYPLRKYA VKA

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Macromolecule #34: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.546292 KDa
SequenceString:
MGFRPGRHRK AAATALRYPM AVGLNKGHKV TKNVSKPRHS RRRGRLTKHT KFVRDMIREV CGFAPYERRA MELLKVSKDK RALKFIKKR VGTHIRAKRK REELSSVLAA MRKAAAKD

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Macromolecule #35: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 11.111032 KDa
SequenceString:
MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

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Macromolecule #36: L38

MacromoleculeName: L38 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 8.107752 KDa
SequenceString:
PRKIEEIKDF LLTARRKDAK SVKIKKNKDN VKFKVRCSRY LYTLVITDKE KAEKLKQSLP PGLAVKELK

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Macromolecule #37: ribosomal protein eL39

MacromoleculeName: ribosomal protein eL39 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 6.295562 KDa
SequenceString:
SSHKTFRIKR FLAKKQKQNR PIPQWIRMKT GNKIRYNSKR RHWRRTKLGL

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Macromolecule #38: 60S ribosomal protein L40

MacromoleculeName: 60S ribosomal protein L40 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.69531 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGLTLSDYN IQKESTLHLV LRLHGGIIEP SLRQLAQKY NCDKMICRKC YARLHPRAVN CRKKKCGHTN NLRPKKKVK

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Macromolecule #39: 60s ribosomal protein l41

MacromoleculeName: 60s ribosomal protein l41 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 3.213075 KDa
SequenceString:
RAKWRKKRMR RLKRKRRKMR QRS

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Macromolecule #40: LOW QUALITY PROTEIN: 60S ribosomal protein L36a

MacromoleculeName: LOW QUALITY PROTEIN: 60S ribosomal protein L36a / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.170214 KDa
SequenceString:
MVAVSHGSEE VHSGTAYKLP LLL(UNK)PLSFRA ASAPANMVNV PKTRRTFCKK CGKHQPHKVT QYKKGKDSLY AQGKRR YDR KQSGYGGQTK PIFRKKAKTT KKIVLRLECV EPNCRSKRML AIKRCKHFEL GGDKKRKGQV IQF

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Macromolecule #41: ribosomal protein eL43

MacromoleculeName: ribosomal protein eL43 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 10.168153 KDa
SequenceString:
AKRTKKVGIV GKYGTRYGAS LRKMVKKIEI SQHAKYTCSF CGKTKMKRRA VGIWHCGSCM KTVAGGAWTY NTTSAVTVKS AIRRLKELK DQ

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Macromolecule #42: eL28

MacromoleculeName: eL28 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.783614 KDa
SequenceString:
MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYHPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

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Macromolecule #43: 60S acidic ribosomal protein P0

MacromoleculeName: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.380504 KDa
SequenceString: MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ...String:
MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ASEATLLNML NISPFSFGLI IQQVFDNGSI YNPEVLDITE DTLHSRFLEG VRNVASVCLQ IGYPTVASVP HS IINGYKR VLALSVETEY TFPLAEKVKA FLADPSAFVA AAPVAAASTA APAAAAAAPA KVEAKEESEE SDEDMGFGLF D

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Macromolecule #44: L12

MacromoleculeName: L12 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.689461 KDa
SequenceString:
MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECP

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Macromolecule #45: 40S ribosomal protein SA

MacromoleculeName: 40S ribosomal protein SA / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 32.927988 KDa
SequenceString: MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA ...String:
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGTTTEWS

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Macromolecule #46: 40S ribosomal protein S3a

MacromoleculeName: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.94201 KDa
SequenceString: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM ...String:
MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLRLFC VGFTKKRNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGGSS GK AAGDETG AKVERADGYE PPVQESV

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Macromolecule #47: 40S ribosomal protein S2

MacromoleculeName: 40S ribosomal protein S2 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 27.956623 KDa
SequenceString: MADDAGAAGG PGGPGARGGK AEDKEWLPVT KLGRLVKDVK IKSLEEIYLF SLPIKESEII DFFLGAALKD EVLKIMPVQK QTRAAQRTR FKAFVAIGDY NGHVGLGVKC SKEVATAIRG AIILAKLSIV PVRRGYWGNK IGKPHTVPCK VTGRCGSVLV R LIPAPRGT ...String:
MADDAGAAGG PGGPGARGGK AEDKEWLPVT KLGRLVKDVK IKSLEEIYLF SLPIKESEII DFFLGAALKD EVLKIMPVQK QTRAAQRTR FKAFVAIGDY NGHVGLGVKC SKEVATAIRG AIILAKLSIV PVRRGYWGNK IGKPHTVPCK VTGRCGSVLV R LIPAPRGT GIVSAPVPKK LLLMAGIDDC YTSAWGCTAT LGNFAKATFD AISKTYSYLT PDLWKETVFT KSPYQEFTDH LV KTHTRVS VQRTQAPAVA TT

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Macromolecule #48: 40S ribosomal protein S3

MacromoleculeName: 40S ribosomal protein S3 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-(apurinic or apyrimidinic site) lyase
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 31.146607 KDa
SequenceString: MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG ...String:
MSARRRRRRA AFRRRAEPFI PISVREPLPF LSAARGGKMA VQISKKRKFV ADGIFKAELN EFLTRELAED GYSGVEVRVT PTRTEIIIL ATRTQNVLGE KGRRIRELTA VVQKRFGFPE GSVELYAEKV ATRGLCAIAQ AESLRYKLLG GLAVRRACYG V LRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPSGKIGP KK PLPDHVS IVEPKDEILP TTPISEQKGG KPEPPAMPQP VPTA

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Macromolecule #49: S4

MacromoleculeName: S4 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 29.497697 KDa
SequenceString: ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIIFLRNK LKYALTGDEV KKICMQRFIK IDGKVRADIT YPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKMNDTI Q IDLETGKI ...String:
ARGPKKHLKR VAAPKHWMLD KLTGVFAPRP STGPHKLREC LPLIIFLRNK LKYALTGDEV KKICMQRFIK IDGKVRADIT YPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF VGTKGIPHLV THDARTIRYP DPLIKMNDTI Q IDLETGKI TDFIKFDTGN LCMVTGGANL GRIGVITNRE RHPGSFDVVH VKDANGNSFA TRLSNIFVIG KGNKPWISLP RG KGIRLTI AEERDKRLAA KQSSG

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Macromolecule #50: Ribosomal protein S5

MacromoleculeName: Ribosomal protein S5 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 23.04465 KDa
SequenceString: MMTEWETAAP AVAETPDIKL FGKWSTDDVQ INDISLQDYI AVKEKYAKYL PHSAGRYAAK RFRKAQCPIV ERLTNSMMMH GRNNGKKLM TVRIVKHAFE IIHLLTGENP LQVLVNAIIN SGPREDSTRI GRAGTVRRQA VDVSPLRRVN QAIWLLCTGA R EAAFRNIK ...String:
MMTEWETAAP AVAETPDIKL FGKWSTDDVQ INDISLQDYI AVKEKYAKYL PHSAGRYAAK RFRKAQCPIV ERLTNSMMMH GRNNGKKLM TVRIVKHAFE IIHLLTGENP LQVLVNAIIN SGPREDSTRI GRAGTVRRQA VDVSPLRRVN QAIWLLCTGA R EAAFRNIK TIAECLADEL INAAKGSSNS YAIKKKDELE RVAKSNR

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Macromolecule #51: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 30.070432 KDa
SequenceString: LFRGASEAVG SLKMKLNISF PATGCQKLIE VDDERKLRTF YEKRMATEVA ADALGEEWKG YVVRISGGND KQGFPMKQGV LTHGRVRLL LSKGHSCYRP RRTGERKRKS VRGCIVDANL SVLNLVIVKK GEKDIPGLTD TTVPRRLGPK RASRIRKLFN L SKEDDVRQ ...String:
LFRGASEAVG SLKMKLNISF PATGCQKLIE VDDERKLRTF YEKRMATEVA ADALGEEWKG YVVRISGGND KQGFPMKQGV LTHGRVRLL LSKGHSCYRP RRTGERKRKS VRGCIVDANL SVLNLVIVKK GEKDIPGLTD TTVPRRLGPK RASRIRKLFN L SKEDDVRQ YVVRKPLNKE GKKPRTKAPK IQRLVTPRVL QHKRRRIALK KQRTKKNKEE AAEYAKLLAK RMKEAKEKRQ EQ IAKRRRL SSLRASTSKS ESSQK

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Macromolecule #52: ribosomal protein eS7

MacromoleculeName: ribosomal protein eS7 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 21.716387 KDa
SequenceString:
SAKIVKPNGE KPDEFESGIS QALLELEMNS DLKAQLRELN ITAAKEIEVG GGRKAIIIFV PVPQLKSFQK IQVRLVRELE KKFSGKHVV FIAQRRILPK PTRKSRTKNK QKRPRSRTLT AVHDAILEDL VFPSEIVGKR IRVKLDGSRL IKVHLDKAQQ N NVEHKVET FSGVYKKLTG KDVNFEFPEF QL

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Macromolecule #53: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.003012 KDa
SequenceString: GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG ...String:
GISRDNWHKR RKTGGKRKPY HKKRKYELGR PAANTKIGPR RIHTVRVRGG NKKYRALRLD VGNFSWGSEC CTRKTRIIDV VYNASNNEL VRTKTLVKNC IVLIDSTPYR QWYESHYALP LGRKKGAKLT PEEEEILNKK RSKKIQKKYD ERKKNAKISS L LEEQFQQG KLLACIASRP GQCGRADGYV LEGKELEFYL RKIKARKG

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Macromolecule #54: 40S ribosomal protein S9

MacromoleculeName: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 22.65559 KDa
SequenceString: MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG ...String:
MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG EDEEED

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Macromolecule #55: S10_plectin domain-containing protein

MacromoleculeName: S10_plectin domain-containing protein / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 11.773953 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSR

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Macromolecule #56: 40S ribosomal protein S11

MacromoleculeName: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.468826 KDa
SequenceString:
MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

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Macromolecule #57: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.766122 KDa
SequenceString:
GGVMDVNTAL QEVLKTALIH DGLARGIREA AKALDKRQAH LCVLASNCDE PMYVKLVEAL CAEHQINLIK VDDNKKLGEW VGLCKIDRE GKPRKVVGCS CVVVKDYGKE SQAKDVIEEY FKCKK

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Macromolecule #58: ribosomal protein uS15

MacromoleculeName: ribosomal protein uS15 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.128191 KDa
SequenceString:
GRMHAPGKGL SQSALPYRRS VPTWLKLTSD DVKEQIYKLA KKGLTPSQIG VILRDSHGVA QVRFVTGNKI LRILKSKGLA PDLPEDLYH LIKKAVAVRK HLERNRKDKD AKFRLILIES RIHRLARYYK TKRVLPPNWK YESSTASALV A

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Macromolecule #59: S14

MacromoleculeName: S14 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 18.133984 KDa
SequenceString:
MKARALSGSG VRRRRAAMAP RKGKEKKEEQ VISLGPQVAE GENVFGVCHI FASFNDTFVH VTDLSGKETI CRVTGGMKVK ADRDESSPY AAMLAAQDVA QRCKELGITA LHIKLRATGG NRTKTPGPGA QSALRALARS GMKIGRIEDV TPIPSDSTRR K GGRRGRRL

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Macromolecule #60: 40S ribosomal protein uS19

MacromoleculeName: 40S ribosomal protein uS19 / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.049182 KDa
SequenceString:
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

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Macromolecule #61: Rps16 protein

MacromoleculeName: Rps16 protein / type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.585736 KDa
SequenceString:
RGVELVLVLG AMPSKGPLQS VQVFGRKKTA TAVAHCKRGN GLIKVNGRPL EMIEPRTLQY KLLEPVLLLG KERFAGVDIR VRVKGGGHV AQIYAIRQSI SKALVAYYQK YVDEASKKEI KDILIQYDRT LLVADPRRCE SKKFGGPGAR ARYQKSYR

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Macromolecule #62: S17

MacromoleculeName: S17 / type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.969445 KDa
SequenceString:
MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK

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Macromolecule #63: ribosomal protein uS13

MacromoleculeName: ribosomal protein uS13 / type: protein_or_peptide / ID: 63 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 16.170774 KDa
SequenceString:
PEKFQHILRV LNTNIDGRRK IAFAITAIKG VGRRYAHVVL RKADIDLTKR AGELTEDEVE RVITIMQNPR QYKIPDWFLN RQKDVKDGK YSQVLANGLD NKLREDLERL KKIRAHRGLR HFWGLRVRGQ HTKTTGRR

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Macromolecule #64: S19

MacromoleculeName: S19 / type: protein_or_peptide / ID: 64 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.683128 KDa
SequenceString:
VTVKDVNQQE FVRALAAFLK KSGKLKVPEW VDTVKLAKHK ELAPYDENWF YTRAASTARH LYLRGGAGVG SMTKIYGGRQ RNGVMPSHF SRGSKSVARR VLQALEGLKM VEKDQDGGRK LTPQGQRDLD RIAGQVAAAK KK

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Macromolecule #65: Ribosomal_S10 domain-containing protein

MacromoleculeName: Ribosomal_S10 domain-containing protein / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 11.76589 KDa
SequenceString:
AIHRIRITLT SRNVKSLEKV CADLIRGAKE KNLKVKGPVR MPTKTLRITT RKTPCGEGSK TWDRFQMRIH KRLIDLHSPS EIVKQITSI SIEPGVEVEV TIADA

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Macromolecule #66: S21

MacromoleculeName: S21 / type: protein_or_peptide / ID: 66 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 9.115383 KDa
SequenceString:
MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIPMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKAHGIVS QNF

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Macromolecule #67: Ribosomal protein S15a

MacromoleculeName: Ribosomal protein S15a / type: protein_or_peptide / ID: 67 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.778584 KDa
SequenceString:
MHEASLAATM VRMNVLADAL KSINNAEKRG KRQVLIRPCS KVIVRFLTVM MKHGYIGEFE IIDDHRAGKI VVNLTGRLNK CGVISPRFD VQLKDLEKWQ NNLLPSRQFG FIVLTTSAGI MDHEEARRKH TGGKILGFFF

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Macromolecule #68: Ribosomal protein S23

MacromoleculeName: Ribosomal protein S23 / type: protein_or_peptide / ID: 68 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 15.626392 KDa
SequenceString:
GKCRGLRTAR KLRSHRRDQK WHDKQYKKAH LGTALKANPF GGASHAKGIV LEKVGVEAKQ PNSAIRKCVR VQLIKNGKKI TAFVPNDGC LNFIEENDEV LVAGFGRKGH AVGDIPGVRF KVVKVANVSL LALYKGKKER PR

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Macromolecule #69: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 69 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 17.007125 KDa
SequenceString:
MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI REKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KEVSWRWITA EGVKILQ

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Macromolecule #70: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 70 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.581994 KDa
SequenceString:
MKDDKKKKDA GKSAKKDKDP VNKSGGKAKK KKWSKGKVRD KLNNLVLFDK ATYDKLCKEV PNYKLITPAV VSERLKIRGS LARAALQEL LSKGLIKLVS KHRAQVIYTR NTKGGDAPAA GEDA

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Macromolecule #71: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 71 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 13.147561 KDa
SequenceString:
MTKKRRNNGR AKKGRGHVQP IRCTNCARCV PKDKAIKKFV IRNIVEAAAV RDISEASVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAVSVAL STADMVGA

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Macromolecule #72: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 9.480186 KDa
SequenceString:
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

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Macromolecule #73: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 73 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 7.776891 KDa
SequenceString:
MDTSHVQPIK LARVTKVIGK TGSQGQCTQV RVEFMDDTSH STIRNVKGPV REGDVLTLLE SEREARRLR

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Macromolecule #74: ribosomal protein uS14

MacromoleculeName: ribosomal protein uS14 / type: protein_or_peptide / ID: 74 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 6.364426 KDa
SequenceString:
QQLYWSHPRK FGQGSRSCRV CSNRHGLIRK YGLNMCRQCF RQYAKDIGFI KLD

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Macromolecule #75: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 75 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 14.498884 KDa
SequenceString:
MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

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Macromolecule #76: S27a

MacromoleculeName: S27a / type: protein_or_peptide / ID: 76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 7.468886 KDa
SequenceString:
KSYTTPKKNK HKRKKVKLAV LKYYKVDENG KISRLRRECP SFMASHFDRH YCGKCCLTYC FN

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Macromolecule #77: ribosomal protein RACK1

MacromoleculeName: ribosomal protein RACK1 / type: protein_or_peptide / ID: 77 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 34.669113 KDa
SequenceString: TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG ...String:
TEQMTLRGTL KGHNGWVTQI ATTPQFPDMI LSASRDKTII MWKLTRDETN YGIPQRALRG HSHFVSDVVI SSDGQFALSG SWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS S NPIIVSCG WDKLVKVWNL ANCKLKTNHI GHTGYLNTVT VSPDGSLCAS GGKDGQAMLW DLNEGKHLYT LDGGDIINAL CF SPNRYWL CAATGPSIKI WDLEGKIIVD ELKQEVISTS SKAEPPQCTS LAWSADGQTL FAGYTDNLVR VWQVTI

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Macromolecule #78: Sec61Beta

MacromoleculeName: Sec61Beta / type: protein_or_peptide / ID: 78 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 1.788032 KDa
SequenceString:
GMWRFYTEDS PGLKV

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Macromolecule #79: P-Site tRNA

MacromoleculeName: P-Site tRNA / type: rna / ID: 79 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 24.436551 KDa
SequenceString:
GUCUCCGUAG UGUAGCGGUU AUCACGUUCG CCUAACACGC GAAAGGUCCU CGGUUCGAAA CCGGGCGGAA ACACCA

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Macromolecule #80: 28S ribosomal RNA+BlaS

MacromoleculeName: 28S ribosomal RNA+BlaS / type: rna / ID: 80 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 1.2267394999999999 MDa
SequenceString: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC ...String:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGA GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUCAACCCG GCGGC GGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCACACA CACACAGCGG GGGACCGUCC CCCGACCGGC GACCGG CCG CCGCCGGGCG CAUUUCCACC GCGGCGGUGC GCCGCGACCG GCUCCGGGAC GGCUGGGAAG GCCCGGCGGG GAAGGUG GC UCGGACACAC ACACACCGAG UGUUACAGCC CCCCCGGCAG CAGCACUCGC CGAAUCCCGG GGCCGAGGGA GCGAGACC C GUCGCCGCGC UCUCCCCCCU CCCGGCGCAC ACACACACAC GCCGACACAC ACACAGGGGG GGCCGGGCCA CCCCUCCCA CGGCGCGACC GCUACACACA CACACGGGGC GGACUGUCCC CAGUGCGCCC CGGGCGGGUC GCGCCGUCGG GCCCGGGGGA ACACACACA CAGGGGCCAC GCGCGCGUCC CCCGAAGAGG GGGACGGCGG AGCGAGCGCA CGGGGUCGGC GGCGACGUCG G CUACCCAC CCGACCCGUC UUGAAACACG GACCAAGGAG UCUAACACGU GCGCGAGUCG GGGGCUCGCA CGAAAGCCGC CG UGGCGCA AUGAAGGUGA AGGCCGGCGC GCUCGCCGGC CGAGGUGGGA UCCCGAGGCC UCUCCAGUCC GCCGAGGGCG CAC CACCGG CCCGUCUCGC CCGCCGCGCC GGGGAGGUGG AGCACGAGCG CACGUGUUAG GACCCGAAAG AUGGUGAACU AUGC CUGGG CAGGGCGAAG CCAGAGGAAA CUCUGGUGGA GGUCCGUAGC GGUCCUGACG UGCAAAUCGG UCGUCCGACC UGGGU AUAG GGGCGAAAGA CUAAUCGAAC CAUCUAGUAG CUGGUUCCCU CCGAAGUUUC CCUCAGGAUA GCUGGCGCUC UCGCAA CAC ACACACACAC GCAGUUUUAU CCGGUAAAGC GAAUGAUUAG AGGUCUUGGG GCCGAAACGA UCUCAACCUA UUCUCAA AC UUUAAAUGGG UAAGAAGCCC GGCUCGCUGG CGUGACAGCC GGGCGUGGAA UGAGAGUGCC UAGUGGGCCA CUUUUGGU A AGCAGAACUG GCGCUGCGGG AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGCCGACGCU CAUCAGACCC CAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAGGAGUGU GUAACAACUC ACCUGCCGAA UCAACUAGC CCUGAAAAUG GAUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGUCGCCG GCAGUCGAGA GUGGACGGGA G CGGCGGGG GCGGCGCGCG CGCGCGCACA CACAGCGCCC GCCCCCGGAG CCCCGCGGAC GCUACGCCGC GACGAGUAGG AG GGCCGCU GCGGUGAGCC UUGAAGCCUA GGGCGCGGGC CCGGGUGGAG CCGCCGCAGG UGCAGAUCUU GGUGGUAGUA GCA AAUAUU CAAACGAGAA CUUUGAAGGC CGAAGUGGAG AAGGGUUCCA UGUGAACAGC AGUUGAACAU GGGUCAGUCG GUCC UGAGA GAUGGGCGAG CGCCGUUCCG AAGGGACGGG CGAUGGCCUC CGUUGCCCUC GGCCGAUCGA AAGGGAGUCG GGUUC AGAU CCCCGAAUCC GGAGUGGCGG AGAUGGGCGC CGCGAGGCGU CCAGUGCGGU AACGCGACCG AUCCCGGAGA AGCCGG CGG GAGCCCCGGG GAGAGUUCUC UUUUCUUUGU GAAGGGCAGG GCGCCCUGGA AUGGGUUCGC CCCGAGAGAG GGGCCCG UG CCUUGGAAAG CGUCGCGGUU CCGGCGGCGU CCGGUGAGCU CUCGCUGGCC CUUGAAAAUC CGGGGGAGAG GGUGUAAA U CUCGCGCCGG GCCGUACCCA UAUCCGCAGC AGGUCUCCAA GGUGAACAGC CUCUGGCAUG UUGGAACAAU GUAGGUAAG GGAAGUCGGC AAGCCGGAUC CGUAACUUCG GGAUAAGGAU UGGCUCUAAG GGCUGGGUCG GUCGACACAC ACACAUCGGC CGGCGCCUA GCAGCCGACU UAGAACUGGU GCGGACCAGG GGAAUCCGAC UGUUUAAUUA AAACAAAGCA UCGCGAAGGC C CGCGGCGG GUGUUGACGC GAUGUGAUUU CUGCCCAGUG CUCUGAAUGU CAAAGUGAAG AAAUUCAAUG AAGCGCGGGU AA ACGGCGG GAGUAACUAU GACUCUCUUA AGGUAGCCAA AUGCCUCGUC AUCUAAUUAG UGACGCGCAU GAAUGGAUGA ACG AGAUUC CCACUGUCCC UACCUACUAU CCAGCGAAAC CACAGCCAAG GGAACGGGCU UGGCGGAAUC AGCGGGGAAA GAAG ACCCU GUUGAGCUUG ACUCUAGUCU GGCACGGUGA AGAGACAUAC ACACACACAG UUUUUUCACU GACCCGGUGA GGCGG GGGG GCGAGCCCCG AGGGGCUCUC GCUUCUGGCG CCAAGCGCCC GGCCGCGCGC CGGCCGGGCG CGACCCGCUC CGGGGA CAG UGCCAGGUGG GGAGUUUGAC UGGGGCGGUA CACCUGUCAA ACGGUAACGC AGGUGUCCUA AGGCGAGCUC AGGGAGG AC AGAAACCUCC CGUGGAGCAG AAGGGCAAAA GCUCGCUUGA UCUUGAUUUU CAGUACGAAU ACAGACCGUG AAAGCGGG G CCUCACGAUC CUUCUGACCU UUUGGGUUUU AAGCAGGAGG UGUCAGAAAA GUUACCACAG GGAUAACUGG CUUGUGGCG GCCAAGCGUU CAUAGCGACG UCGCUUUUUG AUCCUUCGAU GUCGGCUCUU CCUAUCAUUG UGAAGCAGAA UUCACCAAGC GUUGGAUUG UUCACCCACU AAUAGGGAAC GUGAGCUGGG UUUAGACCGU CGUGAGACAG GUUAGUUUUA CCCUACUGAU G AUGUGUUG UUGCCAUGGU AAUCCUGCUC AGUACGAGAG GAACCGCAGG UUCAGACAUU UGGUGUAUGU GCUUGGCUGA GG AGCCAAU GGGGCGAAGC UACCAUCUGU GGGAUUAUGA CUGAACGCCU CUAAGUCAGA AUCCCGCCCA GGCGGAACGA UAC GGCAGC GCCGCGGAGC CUCGGUUGGC CUCGGAUAGC CGGUCCCCCG ACACACACAC ACGGGGUCCG GUGCGGAGUG CCCU UCGUC CUGGGAAACG GGGCGCGGCC GGAGAGGCGG CCGCCCCCUC GCCCGUCACG CACCGGCCGU UCGUGGGGAA CCUGG CGCU AAACCAUUCG UAGACGACCU GCUUCUGGGU CGGGGUUUCG UACGUAGCAG AGCAGCUCCC UCGCUGCGAU CUAUUG AAA GUCAGCCCUC GACACAAGGG UUUGU

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Macromolecule #81: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 81 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 38.691914 KDa
SequenceString:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

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Macromolecule #82: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 82 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 50.143648 KDa
SequenceString:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCU

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Macromolecule #83: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 83 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 573.221562 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGACA CACACACACC ACUUGGAUAA CUGUGGUAAU U CUAGAGCU ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGACA CACACACACC ACUUGGAUAA CUGUGGUAAU U CUAGAGCU AAUACAUGCC GACGGGCGCU GACCCCCUUC GCGGGGGGGA UGCGUGCAUU UAUCAGACAC ACACACAUGG UG ACUCUAG AUAACCUCGG GCCGAUCGCA CGCCCCCCGU GGCGGCGACG ACACAUUCGA ACGUCUGCCC UAUCAACUUU CGA UGGUAG UCGCCGUGCC UACCAUGGUG ACCACGGGUG ACGGGGAAUC AGGGUUCGAU UCCGGAGAGG GAGCCUGAGA AACG GCUAC CACAUCCAAG GAAGGCAGCA GGCGCGCAAA UUACCCACUC CCGACCCGGG GAGGUAGUGA CGAAAAAUAA CAAUA CAGG ACUCUUUCGA GGCCCUGUAA UUGGAAUGAG UCCACUUUAA AUCCUUUAAC GAGGAUCCAU UGGAGGGCAA GUCUGG UGC CAGCAGCCGC GGUAAUUCCA GCUCCAAUAG CGUAUAUUAA AGUUGCUGCA GUUAAAAAGC UCGUAGUUGG AUCUUGG GA GCGGGACACA CACACACGUC CCCGCCACAC GCCUCUCGGC ACACACACAC ACGGGGCCCG AAGCGUUUAC UUUGAAAA A AUUAGAGUGU UCAAAGCAGG CCCGAGCCGC CUGGAUACCG CAGCUAGGAA UAAUGGAAUA GGACCGCGGU UCUAUUUUG UUGGUUUUCG GAACUGAGGC CAUGAUUAAG AGGGACGGCC GGGGGCAUUC GUAUUGCGCC GCUAGAGGUG AAAUUCUUGG ACCGGCGCA AGACGGACCA GAGCGAAAGC AUUUGCCAAG AAUGUUUUCA UUAAUCAAGA ACGAAAGUCG GAGGUUCGAA G ACGAUCAG AUACCGUCGU AGUUCCGACC AUAAACGAUG CCGACCGGCG AUGCGGCGGC GUUAUUCCCA UGACCCGCCG GG CAGCUUC CGGGAAACCA AAGUCUUUGG GUUCCGGGGG GAGUAUGGUU GCAAAGCUGA AACUUAAAGG AAUUGACGGA AGG GCACCA CCAGGAGUGG AGCCUGCGGC UUAAUUUGAC UCAACACGGG AAACCUCACC CGGCCCGGAC ACGGACAGGA UUGA CAGAU UGAUAGCUCU UUCUCGAUUC CGUGGGUGGU GGUGCAUGGC CGUUCUUAGU UGGUGGAGCG AUUUGUCUGG UUAAU UCCG AUAACGAACG AGACUCUGGC AUGCUAACUA GUUACGCGAC CCCCGAGCGG UCGGCGUCCC CCAACUUCUU AGAGGG ACA AGUGGCGUUC AGCCACCCGA GAUUGAGCAA UAACAGGUCU GUGAUGCCCU UAGAUGUCCG GGGCUGCACG CGCGCUA CA CUGACUGGCU CAGCGUGUGC CUACCCUACG CCGGCAGGCG CGGGUAACCC GUUGAACCCC AUUCGUGAUG GGGAUCGG G GAUUGCAAUU AUUCCCCAUG AACGAGGAAU UCCCAGUAAG UGCGGGUCAU AAGCUUGCGU UGAUUAAGUC CCUGCCCUU UGUACACACC GCCCGUCGCU ACUACCGAUU GGAUGGUUUA GUGAGGCCCU CGGAUCGGCC CCGCCGGGGU ACACACACAG CCCUGGCGG AGCGCUGAGA AGACGGUCGA ACUUGACUAU CUAGAGGAAG UAAAAGUCGU AACAAGGUUU CCGUAGGUGA A CCUGCGGA AGGAUCAUU

+
Macromolecule #84: mRNA

MacromoleculeName: mRNA / type: rna / ID: 84 / Number of copies: 1
Source (natural)Organism: Rabbit (rabbit)
Molecular weightTheoretical: 3.22598 KDa
SequenceString:
AAAGUUUAGG

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Macromolecule #85: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 85 / Number of copies: 210 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #86: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 86 / Number of copies: 5 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #87: BLASTICIDIN S

MacromoleculeName: BLASTICIDIN S / type: ligand / ID: 87 / Number of copies: 1 / Formula: BLS
Molecular weightTheoretical: 422.439 Da
Chemical component information

ChemComp-BLS:
BLASTICIDIN S / antibiotic*YM / Blasticidin S

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.63 mg/mL
BufferpH: 7.4
Details: 50 mM HEPES pH 7.4 100 mM KOAc 5 mM Mg(Oac)2 1mM DTT
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 70 % / Chamber temperature: 288.15 K / Instrument: LEICA EM GP
Details: 30s sample incubation on grid followed by 1.1s blotting time..
Details165nM (OD260nm~10.5)

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Calibrated defocus max: 2.0 µm / Calibrated defocus min: 0.4 µm / Calibrated magnification: 79000 / Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
DetailsCollected in super-resolution mode (0.675A pixel size)
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Number grids imaged: 1 / Number real images: 3500 / Average exposure time: 8.0 sec. / Average electron dose: 41.92 e/Å2
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 730463
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final 3D classificationNumber classes: 5 / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionNumber classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.13 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.0) / Number images used: 103842
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

3jah

RefinementProtocol: RIGID BODY FIT
Output model

PDB-7nwi:
Mammalian pre-termination 80S ribosome with Empty-A site bound by Blasticidin S

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