7NE0

Structure of the ternary complex between Netrin-1, Repulsive-Guidance Molecule-B (RGMB) and Neogenin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Netrin-1	polymer	444	49600.8	1	UniProt (O95631) Pfam (PF00055) Pfam (PF00053)	Homo sapiens (Human)	Epididymis tissue protein Li 131P
2	B (B)	Neogenin	polymer	354	39268.2	1	UniProt (Q7TQG5) Pfam (PF00041)	Mus musculus (House mouse)
3	C (C)	Repulsive Guidance Molecule B	polymer	35	3911.2	1	UniProt (Q6NW40)	Homo sapiens (Human)	DRG11-responsive axonal guidance and outgrowth of neurite,DRAGON
4	D (D)	Repulsive Guidance Molecule B C-terminal region (chain D)	polymer	165	18034.4	1	UniProt (Q6NW40) Pfam (PF06535) Pfam (PF06534)	Homo sapiens (Human)	DRG11-responsive axonal guidance and outgrowth of neurite,DRAGON
5	E, F, G, H (I, J, K, L)	1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose-(2-1)-2,3,4,6-tetra-O-sulfonato-alpha-D-glucopyranose	branched		982.8	4	In PDB BIRD (PRD_900013)
6	I, J, K, N (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
7	L (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
8	M (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)

Sequence modifications

A: 24 - 453 (UniProt: O95631)

PDB	External Database	Details
Glu 22	-	expression tag
Thr 23	-	expression tag
Gly 454	-	expression tag
Thr 455	-	expression tag
Lys 456	-	expression tag
Thr 457	-	expression tag
Glu 458	-	expression tag
Thr 459	-	expression tag
Ser 460	-	expression tag
Gln 461	-	expression tag
Val 462	-	expression tag
Ala 463	-	expression tag
Pro 464	-	expression tag
Ala 465	-	expression tag

B: 766 - 1107 (UniProt: Q7TQG5)

PDB	External Database	Details
Glu 763	-	expression tag
Thr 764	-	expression tag
Gly 765	-	expression tag
Gly 1108	-	expression tag
Thr 1109	-	expression tag
Lys 1110	-	expression tag
His 1111	-	expression tag
His 1112	-	expression tag
His 1113	-	expression tag
His 1114	-	expression tag
His 1115	-	expression tag
His 1116	-	expression tag

C: 135 - 168 (UniProt: Q6NW40)

PDB	External Database	Details
Glu 134	-	expression tag
Gly 136	His 136	conflict

D: 169 - 323 (UniProt: Q6NW40)

PDB	External Database	Details
Gly 225	Glu 225	conflict

D: 1330 - 1339 (PDB: 7NE0)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	110814.6
Branched	Number of molecules	4
	Total formula weight	3931.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1021.0
All*	Total formula weight	115766.8

*Water molecules are not included.