7NE0

Structure of the ternary complex between Netrin-1, Repulsive-Guidance Molecule-B (RGMB) and Neogenin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-07
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97630
Spacegroup name	H 3 2
Unit cell lengths	136.780, 136.780, 430.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.960 - 3.250
R-factor	0.25
Rwork	0.249
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bq6 1x5i 4plm
RMSD bond length	0.007
RMSD bond angle	0.970
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.620	3.310
High resolution limit [Å]	3.250	3.250
Rmerge	0.167
Rpim	0.046
Number of reflections	24856	1184
<I/σ(I)>	16.7	1.2
Completeness [%]	99.9	99.6
Redundancy	8.1
CC(1/2)	0.999	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298.15	0.1 M imidazole/MES pH 6.5, 10% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 30 mM sodium nitrate, 30 mM sodium phosphate, 30 mM ammonium sulphate