7MXI

IgE-Fc in complex with DARPins E2_79 and E3_53

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	IgE Fc	polymer	247	27550.9	2	UniProt (P01854) Pfam (PF07654)	Homo sapiens (Human)	Immunoglobulin heavy constant epsilon, Ig epsilon chain C region,Ig epsilon chain C region ND
2	C, E (C, D)	DARPin E3_53	polymer	173	18779.8	2		synthetic construct
3	D, F (E, F)	Anti-IgE Inhibitor E2_79	polymer	143	15525.1	2	UniProt (L7MTK7)	synthetic construct
4	G, I (G, I)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
5	H (H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)

Sequence modifications

A, B: 328 - 545 (UniProt: P01854)

PDB	External Database	Details
Ala 299	-	expression tag
Pro 300	-	expression tag
Met 301	-	expression tag
Ala 302	-	expression tag
Glu 303	-	expression tag
Gly 304	-	expression tag
Gly 305	-	expression tag
Gly 306	-	expression tag
Gln 307	-	expression tag
Asn 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
Gly 317	-	expression tag
Gly 318	-	expression tag
Glu 319	-	expression tag
Asn 320	-	expression tag
Leu 321	-	expression tag
Tyr 322	-	expression tag
Phe 323	-	expression tag
Gln 324	-	expression tag
Gly 325	-	expression tag
Gly 326	-	expression tag
Ser 327	-	expression tag

E, F: 56 - 135 (UniProt: L7MTK7)

PDB	External Database	Details
Met -6	-	initiating methionine
Arg -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
Gly 4	-	expression tag
Ser 5	-	expression tag
Asp 6	-	expression tag
Asp 7	-	expression tag
Asp 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Asp 13	-	expression tag
Leu 14	-	expression tag
Gly 15	-	expression tag
Lys 16	-	expression tag
Lys 17	-	expression tag
Leu 18	-	expression tag
Leu 19	-	expression tag
Glu 20	-	expression tag
Ala 21	-	expression tag
Ala 22	-	expression tag
Arg 23	-	expression tag
Ala 24	-	expression tag
Gly 25	-	expression tag
Gln 26	-	expression tag
Asp 27	-	expression tag
Asp 28	-	expression tag
Glu 29	-	expression tag
Val 30	-	expression tag
Arg 31	-	expression tag
Ile 32	-	expression tag
Leu 33	-	expression tag
Thr 34	-	expression tag
Ala 35	-	expression tag
Asn 36	-	expression tag
Gly 37	-	expression tag
Ala 38	-	expression tag
Asp 39	-	expression tag
Val 40	-	expression tag
Asn 41	-	expression tag
Ala 42	-	expression tag
Asn 43	-	expression tag
Asp 44	-	expression tag
Tyr 45	-	expression tag
Trp 46	-	expression tag
Gly 47	-	expression tag
His 48	-	expression tag
Thr 49	-	expression tag
Pro 50	-	expression tag
Leu 51	-	expression tag
His 52	-	expression tag
Leu 53	-	expression tag
Ala 54	-	expression tag
Ala 55	-	expression tag
Asn 136	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	123711.6
Branched	Number of molecules	3
	Total formula weight	1743.6
All*	Total formula weight	125455.2

*Water molecules are not included.