7MXI
IgE-Fc in complex with DARPins E2_79 and E3_53
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.908, 137.793, 88.250 |
Unit cell angles | 90.00, 105.68, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.740 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.195 | 0.824 |
Number of reflections | 29053 | 2901 |
<I/σ(I)> | 5.07 | 0.66 |
Completeness [%] | 98.7 | 98.37 |
Redundancy | 3.7 | |
CC(1/2) | 0.983 | 0.577 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | E3_53, E2_79, and IgE-Fc3-4 were mixed in equimolar ratios with E3_53 and E2_79, and intact complexes were purified by SEC in 20 mM Tris-HCl, pH 8.0, 50mM NaCl and concentrated to 7mg/ml and mixed 1:1 with acetate buffer pH 4.6, 43% ethylene glycol w/v in hanging drops |