7LFS

Crystal structure of the epidermal growth factor receptor extracellular region with A265V mutation in complex with epiregulin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Isoform 4 of Epidermal growth factor receptor	polymer	507	56550.3	4	UniProt (P00533) Pfam (PF01030) Pfam (PF00757) In PDB	Homo sapiens (Human)	Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1
2	E, F, G, H	Proepiregulin	polymer	48	5480.3	4	UniProt (O14944) In PDB	Homo sapiens (Human)
3	I, K, M	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G81315DD)
4	J	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
5	L	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
6	A, B, C, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B, C, D: 1 - 501 (UniProt: P00533)

PDB	External Database	Details
Val 265	Ala 289	engineered mutation
His 502	-	expression tag
His 503	-	expression tag
His 504	-	expression tag
His 505	-	expression tag
His 506	-	expression tag
His 507	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	248122.2
Branched	Number of molecules	5
	Total formula weight	3581.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	253030.8

*Water molecules are not included.