7L05
Complex of novel maytansinoid M24 bound to T2R-TTL (two tubulin alpha/beta heterodimers, RB3 stathmin-like domain, and tubulin tyrosine ligase)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Tubulin alpha-1B chain | polymer | 451 | 50204.4 | 2 | UniProt (Q2XVP4) Pfam (PF00091) Pfam (PF03953) | Sus scrofa (pig) | Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain |
| 2 | B, D (B, D) | Tubulin beta chain | polymer | 445 | 49907.8 | 2 | UniProt (P02554) Pfam (PF00091) Pfam (PF03953) | Sus scrofa (pig) | Beta-tubulin |
| 3 | E (E) | Stathmin-4 | polymer | 149 | 17673.0 | 1 | UniProt (P63043) Pfam (PF00836) | Rattus norvegicus (Norway rat) | Stathmin-like protein B3,RB3 |
| 4 | F (F) | Tubulin Tyrosine Ligase | polymer | 384 | 44378.5 | 1 | UniProt (E1BQ43) | Gallus gallus (chicken) | |
| 5 | G, S (A, C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) | |||
| 6 | H, N, T, W, Y (A, B, C, D, F) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 7 | I, K, O, U (A, B, C) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 8 | J (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 9 | L, P, Q (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 3 | Chemie (EDO) | |||
| 10 | M, V (B, D) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 11 | R (B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 12 | X (D) | (1S,2R,3S,5S,6S,16E,18E,20R,21S)-11-chloro-21-hydroxy-12,20-dimethoxy-2,5,9,16-tetramethyl-8,23-dioxo-4,24-dioxa-9,22-diazatetracyclo[19.3.1.1~10,14~.0~3,5~]hexacosa-10(26),11,13,16,18-pentaen-6-yl (2S)-2-{methyl[3-(methylamino)propanoyl]amino}propanoate (non-preferred name) | non-polymer | 735.3 | 1 | Chemie (XQ4) | |||
| 13 | Z (F) | PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER | non-polymer | 505.2 | 1 | Chemie (ACP) | |||
| 14 | AA, BA, CA, DA, EA... (A, B, C, D, E...) | water | water | 18.0 | 957 | Chemie (HOH) |
Sequence modifications
E: 5 - 145 (UniProt: P63043)
F: 1 - 378 (UniProt: E1BQ43)
| PDB | External Database | Details |
|---|---|---|
| Met 3 | - | initiating methionine |
| Ala 4 | - | cloning artifact |
| His 146 | - | expression tag |
| His 147 | - | expression tag |
| His 148 | - | expression tag |
| His 149 | - | expression tag |
| His 150 | - | expression tag |
| His 151 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His 379 | - | expression tag |
| His 380 | - | expression tag |
| His 381 | - | expression tag |
| His 382 | - | expression tag |
| His 383 | - | expression tag |
| His 384 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 262276.0 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 3872.0 | |
| All* | Total formula weight | 266147.9 |
