7L05
Complex of novel maytansinoid M24 bound to T2R-TTL (two tubulin alpha/beta heterodimers, RB3 stathmin-like domain, and tubulin tyrosine ligase)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-20 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.088, 158.036, 181.014 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.620 - 2.210 |
R-factor | 0.1873 |
Rwork | 0.186 |
R-free | 0.21670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ihj |
RMSD bond length | 0.009 |
RMSD bond angle | 1.543 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.077 | 0.932 |
Rmeas | 0.081 | 0.984 |
Rpim | 0.023 | 0.311 |
Number of reflections | 150790 | 7363 |
<I/σ(I)> | 33 | 2.2 |
Completeness [%] | 99.4 | 98.2 |
Redundancy | 12.2 | 9.4 |
CC(1/2) | 0.748 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG 3350, MES, CaCl2, MgCl2, glycerol |