7KR5
Cryo-EM structure of the CRAC channel Orai in an open conformation; H206A gain-of-function mutation in complex with an antibody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Calcium release-activated calcium channel protein 1 | polymer | 214 | 24160.2 | 6 | UniProt (Q9U6B8) Pfam (PF07856) | Drosophila melanogaster (Fruit fly) | Protein orai |
| 2 | G, I, K (H, M, O) | 19B5 Fab heavy chain | polymer | 244 | 26886.5 | 3 | Mus musculus | ||
| 3 | H, J, L (L, N, P) | 19B5 Fab light chain | polymer | 232 | 25587.3 | 3 | Mus musculus | ||
| 4 | M (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A, B, C, D, E, F: 133 - 341 (UniProt: Q9U6B8)
| PDB | External Database | Details |
|---|---|---|
| Ala 206 | His 206 | engineered mutation |
| Ser 224 | Cys 224 | engineered mutation |
| Thr 283 | Cys 283 | engineered mutation |
| Glu 342 | - | expression tag |
| Gly 343 | - | expression tag |
| Glu 344 | - | expression tag |
| Glu 345 | - | expression tag |
| Phe 346 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 302382.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 302422.4 |
