7KR5
Cryo-EM structure of the CRAC channel Orai in an open conformation; H206A gain-of-function mutation in complex with an antibody
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.3 |
| Number of Particle | 236132 |
| Software | RELION,cryoSPARC,CTFFIND |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
