7KR4

Human DNA Ligase 1(E346A/E592A) Bound to a nicked DNA substrate control duplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA ligase 1	polymer	647	71916.2	1	UniProt (P18858) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679)	Homo sapiens (Human)	DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1
2	B (B)	DNA (5'-D(*AP*AP*TP*GP*TP*CP*TP*GP*CP*CP*C)-3')	polymer	11	3309.2	1		Saccharomyces cerevisiae
3	C (C)	DNA (5'-D(P*AP*TP*TP*CP*TP*GP*C)-3')	polymer	7	2088.4	1		Saccharomyces cerevisiae
4	D (D)	DNA (5'-D(*GP*CP*AP*GP*AP*AP*TP*GP*GP*GP*CP*AP*GP*AP*CP*AP*TP*T)-3')	polymer	18	5589.6	1		Saccharomyces cerevisiae
5	E, F, G, H (A)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT) PubChem (175)
6	I (C)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (46874558) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (122197642) PubChem (162290139) PubChem (162946266) PubChem (171699505) PubChem (40459109) PubChem (46875232) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (92144436) PubChem (12358354) PubChem (224) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355)
7	J, K, L, M (A, B, C, D)	water	water		18.0	315	Chemie (HOH)

Sequence modifications

A: 262 - 904 (UniProt: P18858)

PDB	External Database	Details
Ser 258	-	expression tag
Asn 259	-	expression tag
Asp 260	-	expression tag
Met 261	-	expression tag
Ala 346	Glu 346	engineered mutation
Ala 592	Glu 592	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	82903.4
Non-Polymers*	Number of molecules	5
	Total formula weight	583.4
All*	Total formula weight	83486.8

*Water molecules are not included.