7KR4
Human DNA Ligase 1(E346A/E592A) Bound to a nicked DNA substrate control duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.374, 96.556, 114.997 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.916 - 2.200 |
R-factor | 0.1648 |
Rwork | 0.162 |
R-free | 0.21530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6p09 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.162 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 42631 | 2078 |
<I/σ(I)> | 15.7 | |
Completeness [%] | 99.7 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES, 100 mM lithium acetate, 12% (w/v) polyethylene glycol 3350 |