7K48

Structure of NavAb/Nav1.7-VS2A chimera trapped in the resting state by tarantula toxin m3-Huwentoxin-IV

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Maltose/maltodextrin-binding periplasmic protein,Ion transport protein,Sodium channel protein type 9 subunit alpha chimera	polymer	611	68519.1	4	UniProt (P0AEX9) UniProt (A8EVM5) UniProt (Q15858) Pfam (PF01547) Pfam (PF00520) In PDB	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Neuroendocrine sodium channel,hNE-Na,Peripheral sodium channel 1,PN1,Sodium channel protein type IX subunit alpha,Voltage-gated sodium channel subunit alpha Nav1.7
2	E, F, G, H	Mu-theraphotoxin-Hs2a	polymer	35	3999.8	4	UniProt (P83303) Pfam (PF07740) In PDB	Haplopelma schmidti	Mu-TRTX-Hs2a,Huwentoxin-4,Huwentoxin-IV,HwTx-IV,Huwentoxin-IVa,HWTX-IVa,Huwentoxin-IVb,HWTX-IVb,Huwentoxin-IVc,HWTX-IVc

Sequence modifications

A, B, C, D: 354 - 719 (UniProt: P0AEX9)

PDB	External Database	Details
Met 353	-	initiating methionine
Asn 720	-	linker
Ala 721	-	linker

A, B, C, D: 722 - 738 (UniProt: A8EVM5)

PDB	External Database	Details
Ala 725	Arg 4	engineered mutation

A, B, C, D: 739 - 779 (UniProt: Q15858)
A, B, C, D: 780 - 803 (UniProt: A8EVM5)
A, B, C, D: 804 - 829 (UniProt: Q15858)
A, B, C, D: 830 - 963 (UniProt: A8EVM5)

PDB	External Database	Details
Ala 833	Leu 109	engineered mutation

E, F, G, H: 1 - 35 (UniProt: P83303)

PDB	External Database	Details
Gly 1	Glu 53	engineered mutation
Gly 4	Glu 56	engineered mutation
Trp 33	Tyr 85	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	290075.4
All*	Total formula weight	290075.4

*Water molecules are not included.