7JL2

Cryo-EM structure of MDA5-dsRNA filament in complex with TRIM65 PSpry domain (Trimer)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (X)	RNA (44-MER)	polymer	44	14208.5	1		Homo sapiens
2	B (Y)	RNA (44-MER)	polymer	44	13973.2	1		Homo sapiens
3	C, E, G (A, C, E)	Interferon-induced helicase C domain-containing protein 1	polymer	739	84901.7	3	UniProt (Q9BYX4) Pfam (PF04851) Pfam (PF18119) Pfam (PF00271) Pfam (PF11648)	Homo sapiens (Human)	Clinically amyopathic dermatomyositis autoantigen 140 kDa,CADM-140 autoantigen,Helicase with 2 CARD domains,Helicard,Interferon-induced with helicase C domain protein 1,Melanoma differentiation-associated protein 5,MDA-5,Murabutide down-regulated protein,RIG-I-like receptor 2,RLR-2,RNA helicase-DEAD box protein 116,IFIH1
4	D, F, H (B, D, F)	Tripartite motif-containing protein 65	polymer	191	21643.4	3	UniProt (Q6PJ69) Pfam (PF00622)	Homo sapiens (Human)	TRIM65
5	I, M, Q (A, C, E)	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
6	J, N, R (A, C, E)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	3	Chemie (ADP)
7	K, O, S (A, C, E)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	3	Chemie (ALF)
8	L, P, T (A, C, E)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

A, C, E: 287 - 1025 (UniProt: Q9BYX4)

PDB	External Database	Details
Arg 843	His 843	conflict
Lys 944	Asn 944	conflict
Thr 946	Ala 946	conflict

B, D, F: 312 - 502 (UniProt: Q6PJ69)

PDB	External Database	Details
Leu 356	Cys 356	conflict
Ser 407	Cys 407	conflict
Ser 421	Cys 421	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	347816.9
Non-Polymers*	Number of molecules	12
	Total formula weight	1859.7
All*	Total formula weight	349676.6

*Water molecules are not included.