7JIJ

ATP-bound AMP-activated protein kinase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (M)	Maltose/maltodextrin ABC transporter substrate-binding protein MalE	polymer	373	40827.1	1	UniProt (A0A6D0N546) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9)	Escherichia coli
2	B (A)	5'-AMP-activated protein kinase catalytic subunit alpha-1	polymer	484	56084.4	1	UniProt (Q13131) Pfam (PF00069) Pfam (PF21147) Pfam (PF16579)	Homo sapiens (Human)	AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1
3	C (B)	5'-AMP-activated protein kinase subunit beta-2	polymer	198	22384.7	1	UniProt (O43741) Pfam (PF16561) Pfam (PF04739)	Homo sapiens (Human)	AMPK subunit beta-2
4	D (G)	5'-AMP-activated protein kinase subunit gamma-1	polymer	306	34893.5	1	UniProt (P54619) Pfam (PF00571)	Homo sapiens (Human)	AMPKg
5	E (C)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)
6	F (G)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
7	G (G)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (40459109) PubChem (46875232) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (177678969) PubChem (6083) PubChem (46874558) PubChem (1549371) PubChem (92144436) PubChem (57358045) PubChem (12358354) PubChem (57879893) PubChem (57879902) PubChem (224) PubChem (122197642) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505)
8	H (G)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

M: 2 - 368 (UniProt: A0A6D0N546)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 84	Asp 108	conflict
Ala 85	Lys 109	conflict
Ala 241	Lys 265	conflict
Asn 369	-	expression tag
Ala 370	-	expression tag
Ala 371	-	expression tag
Glu 372	-	expression tag
Phe 373	-	expression tag

A: 13 - 550 (UniProt: Q13131)

PDB	External Database	Details
Leu 472	Ile 481	conflict
Pro 528	Glu 483	conflict
-	Ala 484	deletion
-	Lys 485	deletion
-	Ser 486	deletion
-	Gly 487	deletion
-	Thr 488	deletion
-	Ala 489	deletion
-	Thr 490	deletion
-	Pro 491	deletion
-	Gln 492	deletion
Pro 530	Ser 494	conflict
-	Val 497	deletion
-	Ser 498	deletion
-	Asn 499	deletion
-	Tyr 500	deletion
-	Arg 501	deletion
-	Ser 502	deletion
-	Cys 503	deletion
-	Gln 504	deletion
-	Arg 505	deletion
-	Ser 506	deletion
-	Asp 507	deletion
-	Ser 508	deletion
-	Asp 509	deletion
-	Ala 510	deletion
-	Glu 511	deletion
-	Ala 512	deletion
-	Gln 513	deletion
-	Gly 514	deletion
-	Lys 515	deletion
-	Ser 516	deletion
-	Ser 517	deletion
-	Glu 518	deletion
-	Val 519	deletion
-	Ser 520	deletion
-	Leu 521	deletion
-	Thr 522	deletion
-	Ser 523	deletion
-	Ser 524	deletion
-	Val 525	deletion
-	Thr 526	deletion
-	Ser 527	deletion
-	Leu 528	deletion
-	Asp 529	deletion
-	Ser 530	deletion
-	Ser 531	deletion
-	Pro 532	deletion
-	Val 533	deletion
-	Asp 534	deletion
-	Leu 535	deletion
-	Thr 536	deletion
-	Pro 537	deletion
-	Arg 538	deletion
-	Pro 539	deletion
-	Gly 540	deletion
-	Ser 541	deletion

B: 76 - 272 (UniProt: O43741)

PDB	External Database	Details
Met 75	-	initiating methionine
Ala 199	Glu 199	conflict
Ala 200	Glu 200	conflict

G: 24 - 327 (UniProt: P54619)

PDB	External Database	Details
Met 22	-	initiating methionine
Gly 23	-	expression tag
Phe 31	Ser 31	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	154189.6
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	3
	Total formula weight	1281.6
All*	Total formula weight	155813.5

*Water molecules are not included.