7JIJ

ATP-bound AMP-activated protein kinase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (M)	Maltose/maltodextrin ABC transporter substrate-binding protein MalE	polymer	373	40827.1	1	UniProt (A0A6D0N546) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9)	Escherichia coli
2	B (A)	5'-AMP-activated protein kinase catalytic subunit alpha-1	polymer	484	56084.4	1	UniProt (Q13131) Pfam (PF00069) Pfam (PF21147) Pfam (PF16579)	Homo sapiens (Human)	AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1
3	C (B)	5'-AMP-activated protein kinase subunit beta-2	polymer	198	22384.7	1	UniProt (O43741) Pfam (PF16561) Pfam (PF04739)	Homo sapiens (Human)	AMPK subunit beta-2
4	D (G)	5'-AMP-activated protein kinase subunit gamma-1	polymer	306	34893.5	1	UniProt (P54619) Pfam (PF00571)	Homo sapiens (Human)	AMPKg
5	E (C)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)
6	F (G)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
7	G (G)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
8	H (G)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

M: 2 - 368 (UniProt: A0A6D0N546)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 84	Asp 108	conflict
Ala 85	Lys 109	conflict
Ala 241	Lys 265	conflict
Asn 369	-	expression tag
Ala 370	-	expression tag
Ala 371	-	expression tag
Glu 372	-	expression tag
Phe 373	-	expression tag

A: 13 - 550 (UniProt: Q13131)

PDB	External Database	Details
Leu 472	Ile 481	conflict
Pro 528	Glu 483	conflict
-	Ala 484	deletion
-	Lys 485	deletion
-	Ser 486	deletion
-	Gly 487	deletion
-	Thr 488	deletion
-	Ala 489	deletion
-	Thr 490	deletion
-	Pro 491	deletion
-	Gln 492	deletion
Pro 530	Ser 494	conflict
-	Val 497	deletion
-	Ser 498	deletion
-	Asn 499	deletion
-	Tyr 500	deletion
-	Arg 501	deletion
-	Ser 502	deletion
-	Cys 503	deletion
-	Gln 504	deletion
-	Arg 505	deletion
-	Ser 506	deletion
-	Asp 507	deletion
-	Ser 508	deletion
-	Asp 509	deletion
-	Ala 510	deletion
-	Glu 511	deletion
-	Ala 512	deletion
-	Gln 513	deletion
-	Gly 514	deletion
-	Lys 515	deletion
-	Ser 516	deletion
-	Ser 517	deletion
-	Glu 518	deletion
-	Val 519	deletion
-	Ser 520	deletion
-	Leu 521	deletion
-	Thr 522	deletion
-	Ser 523	deletion
-	Ser 524	deletion
-	Val 525	deletion
-	Thr 526	deletion
-	Ser 527	deletion
-	Leu 528	deletion
-	Asp 529	deletion
-	Ser 530	deletion
-	Ser 531	deletion
-	Pro 532	deletion
-	Val 533	deletion
-	Asp 534	deletion
-	Leu 535	deletion
-	Thr 536	deletion
-	Pro 537	deletion
-	Arg 538	deletion
-	Pro 539	deletion
-	Gly 540	deletion
-	Ser 541	deletion

B: 76 - 272 (UniProt: O43741)

PDB	External Database	Details
Met 75	-	initiating methionine
Ala 199	Glu 199	conflict
Ala 200	Glu 200	conflict

G: 24 - 327 (UniProt: P54619)

PDB	External Database	Details
Met 22	-	initiating methionine
Gly 23	-	expression tag
Phe 31	Ser 31	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	154189.6
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	3
	Total formula weight	1281.6
All*	Total formula weight	155813.5

*Water molecules are not included.