7JIJ

ATP-bound AMP-activated protein kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-05
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.07823
Spacegroup name	P 32 2 1
Unit cell lengths	126.688, 126.688, 332.384
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.901 - 5.500
R-factor	0.2531
Rwork	0.249
R-free	0.32270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rer
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	6.150
High resolution limit [Å]	5.500	5.500
Rmerge	0.298	3.100
Number of reflections	10563	2894
<I/σ(I)>	4.7
Completeness [%]	99.5
Redundancy	9.7
CC(1/2)	0.979	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M tri-lithium citrate,10% w/v PEG3350, pH7.5,0.01 M barium chloride