7JHE

Room Temperature Structure of SARS-CoV-2 Nsp10/Nsp16 Methyltransferase in a Complex with 2'-O-methylated m7GpppA Cap-1 and SAH Determined by Fixed-Target Serial Crystallography

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	2'-O-methyltransferase	polymer	301	33627.5	1	UniProt (P0DTD1) Pfam (PF06460) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	Non-structural protein 16
2	B	Non-structural protein 10	polymer	142	15075.2	1	UniProt (P0DTD1) Pfam (PF09401) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	A	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)
5	A	7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-(2'-O-METHYL)-ADENOSINE	non-polymer		801.5	1	Chemie (V9G)
6	A	7-METHYL-GUANOSINE-5'-TRIPHOSPHATE	non-polymer		538.2	1	Chemie (MGP)
7	B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
8		water	water		18.0	158	Chemie (HOH)

Sequence modifications

A: 1 - 298 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

B: 1 - 139 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48702.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1890.4
All*	Total formula weight	50593.1

*Water molecules are not included.