7F5H

The crystal structure of RBD-Nanobody complex, DL28 (SC4)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	SARS-CoV-2 Spike Receptor-Binding Domain (RBD)	polymer	213	23747.6	2	UniProt (P0DTC2) Pfam (PF09408) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	S glycoprotein,E2,Peplomer protein
2	C, D	Nanobody DL28	polymer	143	15525.8	2		Vicugna pacos
3	E, F	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	2	In PDB GlyTouCan (G32152BH)
4	A, B, C, D	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	B	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6		water	water		18.0	21	Chemie (HOH)

Sequence modifications

A, B: 330 - 531 (UniProt: P0DTC2)

PDB	External Database	Details
Ala 327	-	expression tag
Gly 328	-	expression tag
Ser 329	-	expression tag
Gly 532	-	expression tag
Thr 533	-	expression tag
Leu 534	-	expression tag
Glu 535	-	expression tag
Val 536	-	expression tag
Leu 537	-	expression tag
Phe 538	-	expression tag
Gln 539	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	78546.9
Branched	Number of molecules	2
	Total formula weight	1465.4
Non-Polymers*	Number of molecules	5
	Total formula weight	463.3
All*	Total formula weight	80475.6

*Water molecules are not included.